##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212801_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 425957 Sequences flagged as poor quality 0 Sequence length 58 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.117589334134664 38.0 37.0 39.0 33.0 40.0 2 36.23839965066897 38.0 35.0 39.0 30.0 40.0 3 36.75420758433363 38.0 36.0 39.0 32.0 40.0 4 36.85593851022521 38.0 37.0 39.0 32.0 40.0 5 35.79385712642356 38.0 35.0 39.0 29.0 40.0 6 36.60729604161922 38.0 36.0 39.0 31.0 40.0 7 36.9479830123698 38.0 37.0 40.0 32.0 40.0 8 36.78581171338891 38.0 37.0 40.0 31.0 40.0 9 36.750815223132854 38.0 37.0 40.0 31.0 40.0 10 35.81199275983256 38.0 35.0 39.0 29.0 40.0 11 36.52524785365659 38.0 35.0 40.0 31.0 40.0 12 36.77681315250131 38.0 36.0 40.0 31.0 40.0 13 36.723871188875876 38.0 36.0 40.0 31.0 40.0 14 36.75738630894668 38.0 36.0 39.0 31.0 40.0 15 36.77888143638912 38.0 36.0 39.0 32.0 40.0 16 37.11776540824543 39.0 36.0 40.0 33.0 40.0 17 36.86047652697338 38.0 36.0 39.0 32.0 40.0 18 36.809410339541316 38.0 36.0 39.0 32.0 40.0 19 36.868681580535124 38.0 36.0 40.0 32.0 40.0 20 36.727632131881855 38.0 36.0 39.0 31.0 40.0 21 36.784217655772764 38.0 36.0 39.0 32.0 40.0 22 36.656901987759326 38.0 36.0 39.0 32.0 40.0 23 36.49880152221938 38.0 35.0 39.0 31.0 40.0 24 36.30972140380367 38.0 35.0 39.0 31.0 40.0 25 36.33442577537169 38.0 35.0 39.0 31.0 40.0 26 36.19591648922309 38.0 35.0 39.0 31.0 40.0 27 36.08488180731858 38.0 35.0 39.0 31.0 40.0 28 35.85294290268736 38.0 35.0 39.0 30.0 40.0 29 35.833088785957266 38.0 35.0 39.0 30.0 40.0 30 35.70322826012954 38.0 35.0 39.0 29.0 40.0 31 35.381019680390274 38.0 35.0 39.0 29.0 40.0 32 35.239960841117764 38.0 35.0 39.0 28.0 40.0 33 35.074685942477764 38.0 34.0 39.0 28.0 40.0 34 34.837816962745066 38.0 33.0 39.0 27.0 40.0 35 34.585674140817034 38.0 33.0 39.0 27.0 40.0 36 35.428094854644954 38.0 35.0 39.0 29.0 40.0 37 35.26317914719091 38.0 35.0 39.0 28.0 40.0 38 35.15821080531603 38.0 35.0 39.0 28.0 40.0 39 35.07922395922593 38.0 35.0 39.0 28.0 40.0 40 34.92403693330547 38.0 34.0 39.0 28.0 40.0 41 34.92789647781349 38.0 35.0 39.0 28.0 40.0 42 34.75097016835033 38.0 35.0 39.0 27.0 40.0 43 34.635843524111586 38.0 34.0 39.0 27.0 40.0 44 34.559039996995004 38.0 34.0 39.0 27.0 40.0 45 34.33718896508333 38.0 33.0 39.0 26.0 40.0 46 34.14852672922384 38.0 33.0 39.0 25.0 40.0 47 33.91646339888768 38.0 33.0 39.0 24.0 40.0 48 33.904649999882615 38.0 33.0 39.0 25.0 40.0 49 33.743206473892904 38.0 33.0 39.0 24.0 40.0 50 33.51183335407095 38.0 33.0 39.0 23.0 40.0 51 33.35280086957134 38.0 33.0 39.0 22.0 40.0 52 33.20601610021669 38.0 33.0 39.0 22.0 40.0 53 33.033104280479016 37.0 33.0 39.0 20.0 40.0 54 32.74759189307841 37.0 32.0 39.0 18.0 40.0 55 32.586737628446066 37.0 32.0 39.0 17.0 40.0 56 32.29182053587569 37.0 32.0 39.0 2.0 40.0 57 32.07406850926267 37.0 32.0 39.0 2.0 40.0 58 31.76952133666074 36.0 31.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1 1 -2.922317251093709 1 2 -5.077396534377659 1 3 -3.8450368230206564 1 4 -4.128612131064386 1 5 -6.1359245530815585 1 6 -4.085647912349586 1 7 -5.222249333050186 1 8 -5.1633146040961755 1 9 -5.055440344863115 1 10 -4.5765758280824365 1 11 -2.8418313457141693 1 12 -2.4703887108360405 1 13 -3.140831510048379 1 14 -2.911918101193912 1 15 -3.0341163443280124 1 16 -3.0603267357562345 1 17 -2.954768378897924 1 18 -2.95086564687859 1 19 -3.0143226607355516 1 20 -3.1896827329487394 1 21 -2.779280019824398 1 22 -2.769594278975383 1 23 -2.867603681509202 1 24 -2.347382633371737 1 25 -2.585147780585544 1 26 -1.901108563343115 1 27 -2.395037316372779 1 28 -2.1957255918881415 1 29 -2.3098464459648085 1 30 -2.379223384775898 1 31 -2.1850654603877757 1 32 -2.1398930393880065 1 33 -2.0955252741015684 1 34 -1.8130512297978996 1 35 -1.8646577368776605 1 36 -2.0417383878334476 1 37 -2.028714827844979 1 38 -1.968106727963395 1 39 -2.386633188640168 1 40 -2.245021080790373 1 41 -1.9813579981038174 1 42 -2.464858133297035 1 43 -1.7835897705506483 1 44 -2.1971291650016127 1 45 -1.890810208605565 1 46 -2.2396508547228464 1 47 -2.3583577927994597 1 48 -2.1715325230015274 1 49 -1.8339776362490632 1 50 -2.6810024965430976 1 51 -2.3497716011270313 1 52 -2.4819293927751858 1 53 -2.291729063147635 1 54 -2.318690142107908 1 55 -2.196704803967908 1 56 -2.77264105500765 1 57 -2.3254479168857323 1 58 -2.384784469361346 2 1 -0.8495102736061497 2 2 -2.025394159840012 2 3 -0.5877556686300451 2 4 -1.2481061718881676 2 5 -3.0958039996316344 2 6 -0.79982664197194 2 7 -2.1406930976056486 2 8 -2.1295505052788783 2 9 -2.1501843971029473 2 10 -1.510673275454458 2 11 -0.8833035590572464 2 12 -0.5608220639571897 2 13 -0.759170246977007 2 14 -0.9998399241082012 2 15 -0.6297599810237529 2 16 -0.6420336630131871 2 17 -0.3142185821035497 2 18 -0.6186678296266663 2 19 -0.5502192770194014 2 20 -0.876006309367753 2 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-0.8864040013754106 117 29 -0.8395028812630301 117 30 -0.8523137231086508 117 31 -0.846157193907132 117 32 -0.8787751461197786 117 33 -0.7839190655211752 117 34 -0.8519594962785462 117 35 -0.9232991739198653 117 36 -0.4959152277357788 117 37 -0.4366673915003645 117 38 -0.43486640707047997 117 39 -0.304317178873859 117 40 -0.27885435599093 117 41 -0.07572493218125231 117 42 0.20227650221046645 117 43 0.07270600900986324 117 44 -0.05913648973129426 117 45 -0.11367382911480917 117 46 -0.04664422758646225 117 47 -0.226582417843062 117 48 -0.3355018288961915 117 49 -0.050615236528102514 117 50 0.5319291393131991 117 51 0.29267695136686456 117 52 0.07849090718993779 117 53 0.10379761979621094 117 54 0.3735927319765011 117 55 0.10647796547750943 117 56 0.6610875811974424 117 57 0.8272552571637988 117 58 0.8386808253718243 118 1 -0.9570027870669833 118 2 -2.1907178546431183 118 3 -0.8524468348872247 118 4 -1.6715119684484705 118 5 -3.4217242278497224 118 6 -0.7585965345092944 118 7 -2.3516828143493598 118 8 -2.0451411764163083 118 9 -2.2092925841282707 118 10 -1.2952327084938062 118 11 -1.07698275431963 118 12 -1.2337517838374055 118 13 -1.3044143342913372 118 14 -0.9216662662710462 118 15 -1.294609466114153 118 16 -0.7374506315062419 118 17 -0.9433940546329964 118 18 -0.9504876747430515 118 19 -0.9296393222746886 118 20 -0.7446688886205166 118 21 -0.8391490480844084 118 22 -0.8663463556246143 118 23 -0.8536802428417758 118 24 -1.2586031317675577 118 25 -0.9609224037121677 118 26 -0.7749713506678688 118 27 -0.6814347145180761 118 28 -0.412365155901675 118 29 -0.606536992266399 118 30 -0.7705300256584167 118 31 -0.2790047211446023 118 32 -0.6454527458003483 118 33 -0.17028464649202135 118 34 -0.4982004408909404 118 35 -0.5347232227476582 118 36 0.031614576610806466 118 37 -0.2207070926012733 118 38 -0.18776127901161033 118 39 -0.24972816512678264 118 40 -0.8206374830230416 118 41 -0.2836565960908999 118 42 -0.1093357845957641 118 43 -0.6109971454023153 118 44 -0.14399331478405486 118 45 -0.12817670972629713 118 46 -0.44061776018585874 118 47 -0.03127125476051873 118 48 -0.24551837102156782 118 49 -0.3992833102261244 118 50 -0.24029577655013412 118 51 -0.7185317646219502 118 52 -0.25178831247808375 118 53 -0.20099817051809055 118 54 0.009644582983291627 118 55 -0.2579978202734452 118 56 0.10016692635744562 118 57 0.12364451048648561 118 58 0.28019157775332104 119 1 -0.6702912690337044 119 2 -2.1362892037250134 119 3 -0.5105045442879401 119 4 -1.592188641161158 119 5 -3.238818813167285 119 6 -0.5382660025797961 119 7 -2.0033018716034974 119 8 -1.3698631437099777 119 9 -1.7775560268921922 119 10 -1.6507749355014596 119 11 -0.7995353800034195 119 12 -0.7456571479355958 119 13 -0.7523378509082619 119 14 -0.7849948543201535 119 15 -0.8392067071047364 119 16 -0.8270714664353918 119 17 -0.7012400328239536 119 18 -0.6358464254566485 119 19 -0.3396463318062075 119 20 -0.4913574269525398 119 21 -0.4567754906387691 119 22 -0.6733457702925989 119 23 -0.9364649779609735 119 24 -0.5816630451962297 119 25 -0.6732570112868004 119 26 -0.9452957402243882 119 27 -0.6979757636353838 119 28 -0.9114871578816874 119 29 -0.8507505156037993 119 30 -0.8841856047396632 119 31 -0.7637318913894973 119 32 -0.7097308724488514 119 33 -0.08452266705813116 119 34 -0.8386974672597916 119 35 -0.5722711874543052 119 36 -0.13037786465726953 119 37 -0.2850358034978413 119 38 -0.25530570282089116 119 39 -0.299098391238374 119 40 -0.23790694531248846 119 41 -0.06206145795977136 119 42 0.2928812507337213 119 43 0.3241827538627646 119 44 0.04665148985235135 119 45 -0.10293516994178731 119 46 0.42257352776135804 119 47 0.7251294592000121 119 48 0.9788010901839286 119 49 0.286971504310344 119 50 0.40776070437599543 119 51 0.8048923553927878 119 52 0.9392539398216968 119 53 0.7359232721446674 119 54 0.8895286727920109 119 55 0.8003258177456729 119 56 0.614261865764739 119 57 0.4640620473226562 119 58 0.9055429576193461 120 1 -1.4836821722099032 120 2 -2.697890907161458 120 3 -2.0586038100189583 120 4 -2.233951562689853 120 5 -4.40867238184974 120 6 -2.0405685146415777 120 7 -3.6221157187842934 120 8 -3.539623766951408 120 9 -3.7368628228938476 120 10 -2.854688783527216 120 11 -1.1641603451488791 120 12 -1.320761289077474 120 13 -1.5139750426040095 120 14 -1.2914545624714648 120 15 -1.320410604053592 120 16 -0.9967982913346276 120 17 -1.2171970751310326 120 18 -1.2521760944863871 120 19 -1.039483254805134 120 20 -1.3201519245824258 120 21 -1.1989110388901238 120 22 -1.0472652795406745 120 23 -1.218115013026754 120 24 -1.1146882503099178 120 25 -1.2225392189945126 120 26 -0.9352829813502055 120 27 -0.7431641471451726 120 28 -1.104230653813211 120 29 -0.7735804507954782 120 30 -1.3018401687833006 120 31 -0.9201083711231703 120 32 -1.124681569117179 120 33 -1.5274076935475875 120 34 -0.7106101999402483 120 35 -0.7169111928620353 120 36 -0.45327032678200396 120 37 -0.4382014407233399 120 38 -0.4184587501639214 120 39 -0.762397616411377 120 40 -0.6475597518423228 120 41 -0.31902180405479186 120 42 -0.429059788058332 120 43 0.10278671333855272 120 44 -0.028980750386267573 120 45 -0.3543694870885332 120 46 -0.15664556154231235 120 47 0.23163141527906106 120 48 -0.03859660645905194 120 49 0.11863041144095376 120 50 0.06560318720221403 120 51 0.16905259333312017 120 52 -0.00830793226847959 120 53 0.2696666920299222 120 54 0.22218040622448143 120 55 -0.13189160698964741 120 56 -0.19861361269303757 120 57 -0.03634775757641506 120 58 -0.03283421498034045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 527.0 3 49.0 4 85.0 5 79.0 6 91.0 7 149.0 8 141.0 9 254.0 10 302.0 11 380.0 12 542.0 13 617.0 14 767.0 15 849.0 16 950.0 17 1133.0 18 1281.0 19 1342.0 20 1513.0 21 1725.0 22 2067.0 23 2484.0 24 2925.0 25 3439.0 26 4267.0 27 5249.0 28 6457.0 29 8219.0 30 9943.0 31 11942.0 32 15073.0 33 19050.0 34 24294.0 35 33596.0 36 48743.0 37 71784.0 38 79041.0 39 64401.0 40 207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 44.80733970799869 42.74938550135342 3.6048239611040556 8.838450829543827 11 26.16555192190761 21.94845958629627 27.887087194247307 23.998901297548812 12 26.046995353991132 16.125571360489438 30.762964336775777 27.064468948743652 13 20.89342351458011 18.27367551184744 35.17420772519292 25.65869324837953 14 19.72405665360588 22.32032810823628 36.46072256119749 21.49489267696035 15 22.240273079207526 25.185640804118727 32.0696690041483 20.50441711252544 16 27.42302021805069 24.86101850895398 26.81591525885303 20.90004601414231 17 27.52057733933711 24.657826901496406 23.976767444371927 23.844828314794555 18 25.110750615672472 24.947588606361677 25.342933676403955 24.598727101561895 19 24.07002650527171 27.189854279697524 25.796273297758205 22.94384591727256 20 25.10570464677862 26.37110841387859 25.95228089615942 22.57090604318337 21 25.150958811486955 23.729645211577107 28.455149033675788 22.664246943260146 22 24.124322699997183 23.567692437716573 28.38203945947469 23.925945402811557 23 24.746794134357582 25.42975738252985 27.67374594424541 22.149702538867157 24 25.00363889058443 28.022274705719397 24.708301835409458 22.26578456828672 25 24.51098235498502 30.19936331452075 22.696006160260684 22.593648170233546 26 25.722805987672437 28.798356325212797 22.54511300264162 22.933724684473145 27 22.32557702737285 26.067480096634917 24.63697342070232 26.969969455289917 28 23.190338767737344 27.55649064144439 24.471959555448063 24.781211035370198 29 25.133488933450426 25.55238824264226 24.94705056377648 24.367072260130833 30 23.23081909611473 24.952203343652087 25.741846383660356 26.075131176572825 31 24.04917917283168 26.99593696423119 25.509993189130793 23.444890673806338 32 27.664944822728337 25.012444235736087 26.26532049776943 21.057290443766142 33 26.14564305805343 25.29994036410764 26.314689682051473 22.239726895787452 34 24.707200015027425 26.542189495829888 28.358394695318957 20.392215793823727 35 22.951335485764602 29.951276783093633 27.122277663633692 19.975110067508073 36 24.595946548930275 30.074211502782934 25.749277846927033 19.580564101359762 37 25.008161472681966 29.382945692738755 25.601893931387266 20.006998903192017 38 24.668481746234963 29.185821707192744 25.1617950025018 20.983901544070495 39 24.367938110190728 27.68272548140041 25.47623353351073 22.473102874898128 40 23.607698703885525 29.44162317315507 25.805315055879962 21.145363067079444 41 22.900234416472102 28.21524035909409 26.908333294185628 21.97619193024818 42 22.3231718061674 28.278883994126286 27.755418502202645 21.64252569750367 43 23.368168297989314 28.100961877328924 27.07846795073609 21.45240187394567 44 23.360971484943864 27.36858176351764 26.21717480152206 23.05327195001644 45 22.921941572442563 26.569788471385152 27.445873007671022 23.062396948501263 46 23.46540630821308 27.586863018235892 26.750591380294527 22.1971392932565 47 22.783801694843557 28.315543586055846 27.337224987427327 21.56342973167327 48 23.738894019395666 28.246377118096433 25.336322343082863 22.67840651942504 49 23.013752077148894 29.02273523197082 25.468019545765436 22.49549314511485 50 20.131391836782665 28.453266579698905 27.18988353371176 24.225458049806672 51 20.743339623395272 29.05303626694615 26.90916696237008 23.294457147288497 52 22.087936357271406 29.32809555807211 27.279583484091667 21.304384600564816 53 22.483400514730942 28.992749109797515 27.19847696050204 21.325373414969505 54 22.227967948250857 28.509038941135824 26.13547951870665 23.127513591906677 55 22.718562564511313 28.32431644459492 25.951475736692743 23.005645254201024 56 21.393333145216666 29.089186113227424 25.81101799335948 23.70646274819643 57 22.80210865895122 28.754226488029456 25.636383301904093 22.807281551115228 58 22.91593672621413 28.997820172457985 26.135121089772685 21.951122011555203 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 495.0 10 990.0 11 2978.5 12 4967.0 13 6143.0 14 7319.0 15 5787.5 16 3705.5 17 3155.0 18 2946.5 19 2738.0 20 2274.0 21 1810.0 22 1848.5 23 2244.5 24 2602.0 25 3136.5 26 3671.0 27 4286.5 28 5358.0 29 5814.0 30 6120.5 31 6427.0 32 6950.5 33 7474.0 34 8332.0 35 9521.5 36 9853.0 37 9676.0 38 9499.0 39 10068.5 40 10638.0 41 12208.0 42 13548.0 43 13318.0 44 14211.0 45 15104.0 46 15769.5 47 16420.0 48 16405.0 49 17217.5 50 18030.0 51 18439.0 52 18848.0 53 19716.0 54 21918.0 55 23252.0 56 23074.0 57 22896.0 58 26145.5 59 31776.5 60 34158.0 61 30348.5 62 26539.0 63 23615.0 64 20691.0 65 17627.5 66 11915.5 67 9267.0 68 7657.5 69 6048.0 70 4809.5 71 3571.0 72 3156.0 73 2138.0 74 1535.0 75 1119.5 76 704.0 77 541.5 78 306.5 79 234.0 80 188.0 81 142.0 82 107.5 83 73.0 84 55.0 85 25.5 86 14.0 87 12.0 88 10.0 89 9.0 90 8.0 91 6.0 92 2.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.3476548102273234E-4 17 7.04296443068197E-4 18 0.0 19 9.390619240909294E-4 20 0.001408592886136394 21 0.00305195125329552 22 2.3476548102273234E-4 23 0.004460544139431914 24 7.04296443068197E-4 25 2.3476548102273234E-4 26 0.01925076944386405 27 0.005634371544545576 28 0.02112889329204591 29 0.018546473000795854 30 0.04577926879943281 31 0.0392058353307963 32 0.013381632418295743 33 0.009155853759886562 34 0.016198818190568533 35 0.01925076944386405 36 0.034510525710341654 37 0.041318724660000895 38 0.06197808699000133 39 0.039440600811819035 40 0.05235270226806931 41 0.051413640343978384 42 0.07794213969954714 43 0.0777073742185244 44 0.05094410938193292 45 0.046718330723523736 46 0.06056949410386494 47 0.10118392232079765 48 0.09343666144704747 49 0.11597414762522977 50 0.12019992628363896 51 0.09813197106750213 52 0.0777073742185244 53 0.11550461666318432 54 0.12207805013182081 55 0.15118896977863963 56 0.16175341642466257 57 0.15564951391807152 58 0.16292724382977625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 425957.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.791032897686854 #Duplication Level Percentage of deduplicated Percentage of total 1 58.6639884631232 13.37012890972563 2 11.178409559126495 5.095350000117383 3 6.213432220848785 4.248316144587364 4 4.272764730119489 3.895228861129175 5 3.1953028430160693 3.6412126106625786 6 2.450556242274413 3.351042476118481 7 2.0292542233209723 3.2374159833034786 8 1.6501854140914711 3.0087544047873376 9 1.4967037494849609 3.070028195334271 >10 8.106716110424392 32.069434238667284 >50 0.4377832715286362 6.944128163171399 >100 0.27812113720642767 11.96623133320969 >500 0.017511330861145445 2.7742236892456282 >1k 0.009270704573547589 3.328504989940299 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2880 0.6761245853454692 No Hit AGTCGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2117 0.49699852332512434 No Hit AGTCGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1602 0.3760943005984172 No Hit AGTCGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 1541 0.36177360625603056 No Hit AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA 1536 0.3605997788509169 No Hit AGTCGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 1190 0.27937092241705147 No Hit AGTCGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 1188 0.27890139145500603 No Hit AGTCGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 1093 0.2565986707578465 No Hit AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 1031 0.24204321093443704 No Hit AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 970 0.2277225165920504 No Hit AGTCGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 953 0.2237315034146639 No Hit AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 931 0.2185666628321638 No Hit AGTCGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT 855 0.20072448627443618 No Hit AGTCGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG 849 0.19931589338829975 No Hit AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 790 0.18546473000795857 No Hit AGTCGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 684 0.16057958901954894 No Hit AGTCGTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG 639 0.15001514237352598 No Hit AGTCGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 635 0.14907608044943504 No Hit AGTCGTGGGCACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGG 628 0.1474327220822759 No Hit AGTCGTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 612 0.1436764743859122 No Hit AGTCGTGGGGTCACGGGATCGTGGGTTCGAGTCCCACCTCGTGTTTTGTTGCGAGATC 596 0.13992022668954845 No Hit AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 570 0.13381632418295744 No Hit AGTCGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT 555 0.13029484196761645 No Hit AGTCGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA 524 0.12301711205591176 No Hit AGTCGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGC 517 0.12137375368875263 No Hit AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 509 0.11949562984057076 No Hit AGTCGTGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC 496 0.11644367858727524 No Hit AGTCGTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG 488 0.11456555473909338 No Hit AGTCGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC 481 0.11292219637193425 No Hit AGTCGTGGGATTTGGAGGACATGATAGCTTGCATAAAAGATGACGCTATAGTTTATGC 475 0.11151360348579786 No Hit AGTCGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 474 0.11127883800477513 No Hit AGTCGTGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTG 467 0.109635479637616 No Hit AGTCGTGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG 464 0.10893118319454781 No Hit AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 459 0.10775735578943414 No Hit AGTCGTGGGGTTGCGAGATCGTGGGTTCGAGTCCCACCTCGCGTCTGGTCACGGGATC 456 0.10705305934636594 No Hit AGTCGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA 447 0.10494017001716135 No Hit AGTCGTGGGAACTTCTAAAATGCATATATTGGGGCTTGGGGGGATGGGTGTTTTTTAA 436 0.10235774972591129 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 9.390619240909294E-4 0.0 0.0 0.0 0.0 11 0.0016433583671591264 0.0 0.0 0.0 0.0 12 0.0016433583671591264 0.0 0.0 0.0 0.0 13 0.0018781238481818587 0.0 0.0 0.0 0.0 14 0.0030519512532955206 0.0 0.0 0.0 0.0 15 0.0032867167343182528 0.0 0.0 0.0 0.0 16 0.0035214822153409853 0.0 0.0 0.0 0.0 17 0.0035214822153409853 0.0 0.0 0.0 0.0 18 0.0035214822153409853 0.0 0.0 0.0 0.0 19 0.00399101317738645 0.0 0.0 0.0 0.0 20 0.0042257786584091826 0.0 0.0 0.0 0.0 21 0.005164840582500112 0.0 0.0 0.0 0.0 22 0.007042964430681971 0.0 0.0 0.0 0.0 23 0.008686322797841097 0.0 0.0 0.0 0.0 24 0.010329681165000224 0.0 0.0 0.0 0.0 25 0.011503508570113884 0.0 0.0 0.0 0.0 26 0.013851163380341208 0.0 0.0 0.0 0.0 27 0.015494521747500335 0.0 0.0 0.0 0.0 28 0.019955065886932247 0.0 0.0 0.0 0.0 29 0.02230272069715957 0.0 0.0 0.0 0.0 30 0.024650375507386895 0.0 0.0 0.0 0.0 31 0.02605896839352329 0.0 0.0 0.0 0.0 32 0.027702326760682416 0.0 0.0 0.0 0.0 33 0.028641388684773346 0.0 0.0 0.0 0.0 34 0.030284747051932473 0.0 0.0 0.0 0.0 35 0.03662341503954625 0.0 0.0 0.0 0.0 36 0.037562476963637174 0.0 0.0 0.0 0.0 37 0.038736304368750835 0.0 0.0 0.0 0.0 38 0.03967536629284177 0.0 0.0 0.0 0.0 39 0.04084919369795543 0.0 0.0 0.0 0.0 40 0.04319684850818275 0.0 0.0 0.0 0.0 41 0.04577926879943281 0.0 0.0 0.0 0.0 42 0.04695309620454647 0.0 0.0 0.0 0.0 43 0.04836168909068286 0.0 0.0 0.0 0.0 44 0.0485964545717056 0.0 0.0 0.0 0.0 45 0.05047457841988745 0.0 0.0 0.0 0.0 46 0.05164840582500112 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTGCG 50 3.6379788E-12 52.026554 47 CTCGCAC 25 1.6484362E-5 52.026554 46 ATCGCAT 20 3.4365253E-4 52.026554 47 CGTAGCG 30 7.9615893E-7 52.02044 41 AACCGTA 15 0.0072091715 52.01433 37 ACGTCCA 15 0.0072091715 52.01433 40 TTCGCAG 15 0.0072091715 52.01433 40 GTTTCGC 25 1.6530275E-5 52.002113 31 CCGACCG 15 0.007215872 52.002113 32 CGGCGTG 15 0.007219224 51.996006 20 TGCGGTC 15 0.007219224 51.996006 26 AGACGCA 20 3.4505024E-4 51.9838 19 CCAATCG 15 0.0072259307 51.983795 19 CTAACCG 15 0.0072259307 51.983795 17 ATTACAC 15 0.0072292862 51.977695 11 TCAATAC 15 0.0072292862 51.977695 12 GACGCAT 15 0.0072292862 51.977695 9 CGTGGGA 18420 0.0 51.31457 4 AGTCGTG 43170 0.0 51.28538 1 GTCGTGG 43255 0.0 51.184605 2 >>END_MODULE