FastQCFastQC Report
Tue 31 May 2016
SRR212800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424941
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27990.6586796755314267No Hit
AGTCGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19460.4579459266109883No Hit
AGTCGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT15680.36899240129806254No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA14360.33792926547450114No Hit
AGTCGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG13990.3292221743724423No Hit
AGTCGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA11440.2692138438041987No Hit
AGTCGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT11350.2670959027253195No Hit
AGTCGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10790.25391760267896013No Hit
AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT10410.24497518479035915No Hit
AGTCGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG9780.23014959723820483No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8540.2009690757069805No Hit
AGTCGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG8540.2009690757069805No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC8260.1943799256838008No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8020.18873208280678963No Hit
AGTCGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT7960.18732012208753687No Hit
AGTCGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6990.16449342379295007No Hit
AGTCGTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6260.14731456837537446No Hit
AGTCGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA6100.14354933979070034No Hit
AGTCGTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG6010.14143139871182117No Hit
AGTCGTGGGCACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGG5870.1381368237002313No Hit
AGTCGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGC5790.1362542094078943No Hit
AGTCGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT5660.13319496118284654No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5460.12848842545200392No Hit
AGTCGTGGGGTCACGGGATCGTGGGTTCGAGTCCCACCTCGTGTTTTGTTGCGAGATC5370.12637048437312476No Hit
AGTCGTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG5140.12095796828265572No Hit
AGTCGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC4960.11672208612489735No Hit
AGTCGTGGGATTTGGAGGACATGATAGCTTGCATAAAAGATGACGCTATAGTTTATGC4860.11436881825947602No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4830.11366283789984961No Hit
AGTCGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4640.10919162895554912No Hit
AGTCGTGGGGTTGCGAGATCGTGGGTTCGAGTCCCACCTCGCGTCTGGTCACGGGATC4460.10495574679779075No Hit
AGTCGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA4420.10401443965162221No Hit
AGTCGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT4400.10354378607853795No Hit
AGTCGTGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC4360.10260247893236943No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4320.10166117178620092No Hit
AGTCGTGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTG4270.10048453785349025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC150.00711943152.17931426
ATTCGCT150.007209713652.01331349
GTCCGAT150.00721643552.0010648
ACGGTCT203.4448397E-452.0010647
CGGCGAG150.007226525351.9826930
TCGTTGT203.4508546E-451.9826929
TACGGTA203.4508546E-451.9826932
CCGCTAA150.007226525351.9826930
ATTGCGG150.007226525351.9826940
CAATCGG203.4508546E-451.9826930
ACGGTAG203.4508546E-451.9826933
TTACGGT203.4528613E-451.9765731
TCGCACA203.4608977E-451.952119
TAACACG203.4608977E-451.952118
TCGCTAT150.007243366451.952120
CGTCAAC203.4608977E-451.952119
GGATACT353.907553E-851.9459888
ATCGTAA150.007246738351.94598811
TATTCCG203.462909E-451.94598814
GATGCGC203.462909E-451.9459889
Produced by FastQC (version 0.11.5)