FastQCFastQC Report
Tue 31 May 2016
SRR212799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212799_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421589
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26040.6176631743238082No Hit
AGTCGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18110.42956528751936124No Hit
AGTCGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT15010.3560339572427174No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA14020.3325513711221118No Hit
AGTCGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG12850.3047992238886688No Hit
AGTCGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA10750.2549876775722327No Hit
AGTCGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT10700.2538016883742223No Hit
AGTCGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10210.24217899423372052No Hit
AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT8770.20802250533102146No Hit
AGTCGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG8380.19877178958654043No Hit
AGTCGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG8270.19616261335091761No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC7830.1857259084084262No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7690.18240513865399713No Hit
AGTCGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT7330.17386601642832236No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6890.16342931148583098No Hit
AGTCGTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6670.1582109590145853No Hit
AGTCGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6090.14445348431766483No Hit
AGTCGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5650.13401677937517345No Hit
AGTCGTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG5350.12690084418711114No Hit
AGTCGTGGGGTCACGGGATCGTGGGTTCGAGTCCCACCTCGTGTTTTGTTGCGAGATC5230.12405447011188622No Hit
AGTCGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT5200.12334287659307999No Hit
AGTCGTGGGCACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGG5190.1231056787534779No Hit
AGTCGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA5130.12168249171586545No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4910.11646413924461976No Hit
AGTCGTGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC4890.1159897435654156No Hit
AGTCGTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG4860.11527815004660938No Hit
AGTCGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGC4570.10839941269814915No Hit
AGTCGTGGGATTTGGAGGACATGATAGCTTGCATAAAAGATGACGCTATAGTTTATGC4380.10389265374570968No Hit
AGTCGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4330.10270666454769929No Hit
AGTCGTGGGGTTGCGAGATCGTGGGTTCGAGTCCCACCTCGCGTCTGGTCACGGGATC4310.10223226886849515No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4270.10128347751008684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACG150.00702551752.354728
ACCGTAG353.846617E-852.04939339
AACGCCA150.0072306251.9751630
CGCTATA505.456968E-1251.96898344
CACCGTA150.00723401951.96898335
CGTAGCG353.8975486E-851.96280741
TTGGTAG150.007240820751.95663523
CTACCGC150.007240820751.95663523
TCATTCG203.473596E-451.91346447
TAATACG150.00726466451.9134647
ATACGTC150.00726466451.9134649
AATACGT150.00726807551.907348
TACCGGC203.4776694E-451.90114216
TATAGGC505.456968E-1251.89497812
GTCGAAT203.4837867E-451.88266822
ATTCGTT203.4858278E-451.87651418
CGTTTAG251.6768243E-551.87651432
ACTGCGT150.007285147451.8765118
TAGCGTC150.007285147451.8765131
CTGCGTA150.007285147451.8765119
Produced by FastQC (version 0.11.5)