FastQCFastQC Report
Tue 31 May 2016
SRR212798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212798_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416553
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27770.6666618653568693No Hit
AGTCGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20270.48661274795764287No Hit
AGTCGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC13100.31448579172398233No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12710.30512323761922255No Hit
AGTCGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG11890.2854378674502404No Hit
AGTCGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC10660.2559098121967673No Hit
AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9940.23862509692644152No Hit
AGTCGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA9890.23742476947711338No Hit
AGTCGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG8270.19853416011888042No Hit
AGTCGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA8040.1930126538519708No Hit
AGTCGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7720.18533055817627048No Hit
AGTCGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG7710.18509049268640484No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC7560.18148951033842034No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA7430.17836865897016704No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7420.17812859348030144No Hit
AGTCGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6650.1596435507606475No Hit
AGTCGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC6630.15916341978091622No Hit
AGTCGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT6510.1562826339025286No Hit
AGTCGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA5730.13755752569300905No Hit
AGTCGTGGGGCAGGAAAGTCACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCT5580.13395654334502452No Hit
AGTCGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC5350.1284350370781149No Hit
AGTCGTGGGCACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGA4900.11763209003416131No Hit
AGTCGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG4870.11691189356456441No Hit
AGTCGTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4870.11691189356456441No Hit
AGTCGTGGGATTTGGAGGACATGATAGCTTGCATAAAAGATGACGCTATAGTTTAT4830.11595163160510187No Hit
AGTCGTGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT4750.11403110768617677No Hit
AGTCGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4620.11091025631792353No Hit
AGTCGTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC4620.11091025631792353No Hit
AGTCGTGGGAACTTCTAAAATGCATATATTGGGGCTTGGGGGGATGGGTGTTTTTT4480.10754933945980463No Hit
AGTCGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGA4330.1039483571118201No Hit
AGTCGTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA4210.10106757123343248No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4200.10082750574356686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA204.0919537E-450.2180743
TCGCGAC204.0919537E-450.2180744
AGTTTCG150.00828349450.21806745
GTTTCGA150.00828349450.21806746
TGTTGCG355.1004463E-850.21806747
AATCGTC150.00830728350.18165240
AATTGCG402.593879E-950.17558739
GCGTCTA150.00831125250.17558739
CATTACG150.00832316950.15740636
TCACCGT204.1336418E-450.1150335
GCGCTTA150.00835102650.11502535
CGTTTAG150.00838294650.0666832
CCGTCCA150.00839094150.05460731
TACTAGA150.00839094150.05460731
GTACGCC150.00847925349.92220322
TGGTCGG150.00851153849.87423320
AGACGCA204.2431094E-449.85028519
ACTAGTA204.2481394E-449.83831816
CGCACCT150.00853581149.83831411
CGTGGGA181850.049.1668624
Produced by FastQC (version 0.11.5)