FastQCFastQC Report
Tue 31 May 2016
SRR212797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212797_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380931
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24720.6489364215566599No Hit
AGTCGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17700.46465107854178317No Hit
AGTCGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC13370.3509821988759119No Hit
AGTCGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG11620.30504212048901247No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11290.2963791342789114No Hit
AGTCGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA9750.25595186529843983No Hit
AGTCGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC9560.25096408535929077No Hit
AGTCGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC9510.24965151169109365No Hit
AGTCGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA9420.24728887908833883No Hit
AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8800.23101296560269444No Hit
AGTCGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7810.20502400697239134No Hit
AGTCGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG7430.19504844709409316No Hit
AGTCGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG7340.19268581449133831No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7240.19006066715494407No Hit
AGTCGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA6860.18008510727664592No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA6830.17929756307572764No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC6780.1779849894075305No Hit
AGTCGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT6400.1680094295292323No Hit
AGTCGTGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT5990.15724632545001588No Hit
AGTCGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5540.14543316243624174No Hit
AGTCGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC5540.14543316243624174No Hit
AGTCGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG5080.13335748468882816No Hit
AGTCGTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4870.12784467528240023No Hit
AGTCGTGGGGCAGGAAAGTCACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCT4570.11996923327321746No Hit
AGTCGTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC4420.11603151226862607No Hit
AGTCGTGGGCACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGA4300.11288133546495298No Hit
AGTCGTGGGATTTGGAGGACATGATAGCTTGCATAAAAGATGACGCTATAGTTTAT4120.10815607025944331No Hit
AGTCGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4060.10658098185760677No Hit
AGTCGTGGGGTCACGGGATCGTGGGTTCGAGTCCCACCTCGTGTTTTGTTGCGAGA3970.10421834925485192No Hit
AGTCGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGA3950.10369331978757308No Hit
AGTCGTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA3870.10159320191845767No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3820.10028062825026055No Hit
AGTCGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG3810.10001811351662113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATA150.00822669650.30470749
CGACTGA150.00822669650.30470750
CGCATCG252.011197E-550.30470349
TCGGGTA150.00825258950.26473648
GTCCGAT150.00825258950.26473648
GTTTCGA150.0082569150.2580846
CGATTAT150.0082569150.2580846
CCGATTA150.00826123450.25142345
CGCTATA750.050.25142344
CGTAACG150.00826988550.23812542
CGTAGCG150.00828287450.2181841
GGTCGTA150.00830021650.1916239
AATTGCG150.00830021650.1916239
AACGATT204.1021567E-450.19161639
ACTTCGC204.1101943E-450.17171538
TAACGAT204.1101943E-450.17171538
CTCGCGG150.008313240550.17171538
TTCGTCC150.0083436950.1253335
ATCGGTC204.1640986E-450.0394231
TTTTCGA252.0751113E-550.0394231
Produced by FastQC (version 0.11.5)