##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212797_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 380931 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.33592697890169 38.0 37.0 39.0 33.0 40.0 2 36.93688883288574 38.0 37.0 39.0 33.0 40.0 3 36.44661894148809 38.0 36.0 39.0 31.0 40.0 4 37.15506745316081 38.0 38.0 39.0 33.0 40.0 5 36.951825921229826 38.0 37.0 39.0 33.0 40.0 6 36.52594826884659 38.0 36.0 39.0 31.0 40.0 7 37.83228983726712 38.0 38.0 40.0 35.0 40.0 8 37.72736794852611 38.0 38.0 40.0 35.0 40.0 9 37.74972370324284 38.0 38.0 40.0 35.0 40.0 10 36.265523152486935 38.0 35.0 39.0 31.0 40.0 11 36.72829987583053 38.0 35.0 40.0 31.0 40.0 12 37.03085335664464 38.0 36.0 40.0 32.0 40.0 13 37.0827052668331 38.0 36.0 40.0 33.0 40.0 14 37.13154350787938 38.0 36.0 40.0 33.0 40.0 15 37.154059396583634 38.0 36.0 40.0 33.0 40.0 16 37.39087393779976 39.0 37.0 40.0 33.0 40.0 17 37.15335848224482 39.0 36.0 40.0 33.0 40.0 18 37.06210573568441 38.0 36.0 40.0 33.0 40.0 19 37.10586169148743 38.0 36.0 40.0 33.0 40.0 20 36.93896007413416 38.0 36.0 40.0 33.0 40.0 21 36.78473004297366 38.0 36.0 40.0 33.0 40.0 22 36.706337893214254 38.0 36.0 39.0 32.0 40.0 23 36.47336656769861 38.0 36.0 39.0 31.0 40.0 24 36.28845381447034 38.0 35.0 39.0 31.0 40.0 25 36.260811013018106 38.0 35.0 39.0 31.0 40.0 26 35.77659996167285 38.0 35.0 39.0 30.0 40.0 27 35.64506957953015 38.0 35.0 39.0 30.0 40.0 28 35.367536377979214 38.0 35.0 39.0 29.0 40.0 29 35.234612042600894 38.0 35.0 39.0 29.0 40.0 30 35.02048927496056 38.0 34.0 39.0 28.0 40.0 31 34.468835563396 38.0 33.0 39.0 27.0 40.0 32 34.23580648463895 38.0 33.0 39.0 26.0 40.0 33 34.040868293732984 38.0 33.0 39.0 26.0 40.0 34 34.3823369586618 38.0 33.0 39.0 27.0 40.0 35 34.01152439680677 38.0 33.0 39.0 25.0 40.0 36 33.954143926327866 38.0 33.0 39.0 25.0 40.0 37 33.68192927328046 38.0 33.0 39.0 23.0 40.0 38 33.535188787470695 38.0 33.0 39.0 23.0 40.0 39 33.30139054054409 37.0 33.0 39.0 22.0 40.0 40 33.051652398990896 37.0 33.0 39.0 21.0 40.0 41 32.386198550393644 36.0 32.0 39.0 15.0 40.0 42 32.13068508470038 36.0 31.0 39.0 13.0 40.0 43 32.01313098697664 36.0 31.0 39.0 12.0 40.0 44 31.7917365612145 36.0 31.0 39.0 9.0 40.0 45 31.338145228400943 36.0 30.0 39.0 2.0 40.0 46 30.784882301519172 36.0 30.0 38.0 2.0 40.0 47 30.47371046199968 35.0 30.0 38.0 2.0 40.0 48 30.353331705741986 35.0 29.0 38.0 2.0 40.0 49 29.995138227132998 35.0 29.0 38.0 2.0 40.0 50 29.56770123723194 35.0 29.0 38.0 2.0 40.0 51 28.991552275871484 35.0 28.0 38.0 2.0 40.0 52 28.710217860977448 35.0 28.0 38.0 2.0 39.0 53 28.39680677078001 34.0 27.0 38.0 2.0 39.0 54 28.005783199582076 34.0 26.0 38.0 2.0 39.0 55 27.542040422018687 34.0 23.0 38.0 2.0 39.0 56 15.848620878846825 20.0 2.0 23.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.9810323973223731 1 2 -1.5596481915319202 1 3 -1.5562199540279664 1 4 -1.5367395593653441 1 5 -1.8983778658842638 1 6 -1.4618847114982145 1 7 -1.1581775186572614 1 8 -1.304912669181384 1 9 -1.4466187323738424 1 10 -1.3492276413561086 1 11 -0.352678970994333 1 12 -0.6001635695901228 1 13 -0.4681188099493312 1 14 -0.7210876020591712 1 15 -0.5957866491057331 1 16 -0.6423348827052067 1 17 -0.7277726715617447 1 18 -0.5224049008926457 1 19 -0.7177058307620996 1 20 -0.5177014900311292 1 21 -0.4680959232353956 1 22 -0.13598246072912445 1 23 -0.0735488901068777 1 24 -0.3219626049974522 1 25 -0.35194421933051245 1 26 -0.051714087989630286 1 27 0.140378102590077 1 28 0.2955280188243279 1 29 -0.011627480024330339 1 30 -0.29067938181440667 1 31 0.07155230076497787 1 32 0.11912273942115092 1 33 -0.09087740954805668 1 34 -0.31292699498542476 1 35 -0.14623698751735503 1 36 -0.06312794296751889 1 37 -0.16743862371518503 1 38 -0.13269144084250684 1 39 -0.11567795296797101 1 40 -0.10789496938418353 1 41 0.3443988867397678 1 42 0.6505471609221871 1 43 0.6748658519871071 1 44 0.831065801573871 1 45 0.6667804914216902 1 46 1.1240521656859563 1 47 0.9110870817222612 1 48 1.290520532776025 1 49 1.2646529200928924 1 50 1.4820929225566104 1 51 1.22832176935648 1 52 1.6916265954258343 1 53 1.3843935180376121 1 54 1.494308755077597 1 55 1.5664225216348555 1 56 1.4911573837225998 2 1 -0.6567596967640412 2 2 -0.7628512476588654 2 3 -0.9551326780949623 2 4 -1.0681456716192415 2 5 -0.6456596760517641 2 6 -1.534430994189961 2 7 -0.5907737572702487 2 8 -0.6651830188728383 2 9 -0.5462734488005268 2 10 -1.2483607591933108 2 11 -1.2783695381409714 2 12 -1.0056146421731427 2 13 -0.7372930679569762 2 14 -0.5872718512510602 2 15 -0.5299256441101363 2 16 -0.03665606989297743 2 17 -0.3892845728253391 2 18 -0.21517598522999748 2 19 -0.38447045021552384 2 20 0.05032672037145858 2 21 0.18449003033498457 2 22 0.4437266194942069 2 23 0.5204857851796305 2 24 0.12099214081506915 2 25 0.6367055015040393 2 26 0.3339015452751397 2 27 0.4348241795809642 2 28 0.386513913623034 2 29 0.0876966457470445 2 30 0.896361464497673 2 31 0.7447083395542862 2 32 0.6652952342786307 2 33 0.5145075449038998 2 34 0.5893651002246827 2 35 0.4268235473048563 2 36 0.7537982985840515 2 37 0.539621323390314 2 38 0.6505880184581159 2 39 0.4084184325741944 2 40 0.13835833652236573 2 41 0.903537881744171 2 42 1.2358395499906578 2 43 0.5087624138442877 2 44 1.1836223693082246 2 45 0.5898630656209285 2 46 0.9066337113809304 2 47 0.796335392036692 2 48 0.7058893250181661 2 49 0.6118098404572763 2 50 0.2329744976374215 2 51 0.41653804910964 2 52 0.8118881293664728 2 53 0.810598337314719 2 54 0.27571486733149086 2 55 1.181321140500561 2 56 1.0514350798730572 3 1 -0.29392634086855196 3 2 -0.20640072647299945 3 3 -0.17721200664512082 3 4 -0.18558170789918904 3 5 -0.29074563787659 3 6 -0.11805763558700733 3 7 -0.11703611005216175 3 8 -0.05285731154916107 3 9 -0.08593772387836651 3 10 -0.13177080386638096 3 11 -0.5473555947268665 3 12 -0.26664753780059414 3 13 -0.33767211222392746 3 14 -0.2155038804916174 3 15 -0.05067154907832361 3 16 -0.28672375636922 3 17 -0.3102267685746227 3 18 -0.26397793460051133 3 19 -0.26984340269962104 3 20 -0.38540497043671706 3 21 0.16563934670157465 3 22 0.15402850117824585 3 23 -0.01586185256234529 3 24 0.2494610727224682 3 25 0.0430612616231727 3 26 -0.0765154034185116 3 27 0.10749238047442589 3 28 0.2799415567799315 3 29 0.12553338596634944 3 30 0.07921648006556126 3 31 -0.2053728842172049 3 32 -0.09350400763283062 3 33 -0.3214542799235005 3 34 -0.43391904760257916 3 35 -0.2226620354757003 3 36 0.2136602181725209 3 37 0.35561563772630933 3 38 0.2671441305469173 3 39 0.3618501369196707 3 40 0.10079234220803102 3 41 0.3231601912067461 3 42 0.3702648918073592 3 43 0.09333666590872625 3 44 0.11854182240149669 3 45 -0.2482647264462159 3 46 -0.31493796859740897 3 47 -0.39701102734871796 3 48 -0.36308319427248037 3 49 -0.27244628516882585 3 50 0.17043219358977169 3 51 0.2912842248237304 3 52 0.10030020463383593 3 53 0.29029239444210475 3 54 0.4268587687799794 3 55 0.4850303593986247 3 56 0.5082511080306293 4 1 -0.432340279966418 4 2 -0.3023134442714053 4 3 -0.763802138545465 4 4 -0.5927222387573607 4 5 -0.35755071848235076 4 6 -0.6484435662171961 4 7 -0.2455493815063221 4 8 -0.22619227328177516 4 9 -0.2512935291218099 4 10 -0.7229285957569545 4 11 0.08891699931319152 4 12 -0.16202288383544072 4 13 -0.06827787675739216 4 14 0.02764870052124735 4 15 -0.02787395910926449 4 16 -0.052152839580422494 4 17 -0.13615902716443173 4 18 -0.031100553066558234 4 19 -0.34612576713537635 4 20 -0.08585278023968357 4 21 0.05233532455534373 4 22 0.462479893459637 4 23 0.3545175644707115 4 24 0.35197694961548365 4 25 0.613953624430529 4 26 0.5500356468990262 4 27 0.7214314783412235 4 28 0.627067432044548 4 29 0.7272024952319427 4 30 0.2916818765807818 4 31 0.8935035202771786 4 32 1.0007151749107237 4 33 1.0923056940221088 4 34 1.1113748784680908 4 35 0.9619903012772681 4 36 1.511315323204272 4 37 1.4257833628525063 4 38 1.5454457100942207 4 39 1.095916249930582 4 40 1.4086656527437782 4 41 1.399312283699821 4 42 0.8145980623007674 4 43 1.7237877325102602 4 44 1.1867391843946606 4 45 1.0087211064800172 4 46 1.8500595888036635 4 47 1.7961171276748935 4 48 1.415405651192426 4 49 1.5830251364237498 4 50 1.7755535093363939 4 51 1.6162150178541808 4 52 1.2986962313395836 4 53 1.6869297499216707 4 54 1.3473928837449698 4 55 1.7392397715464831 4 56 0.6239163091379414 5 1 -0.2508711854538461 5 2 -0.28732521189186855 5 3 -0.09973673534962302 5 4 -0.3029910359826786 5 5 -0.3444205314391269 5 6 -0.17268486437153996 5 7 0.0011494912795058099 5 8 0.05903007281013828 5 9 0.031327553499274075 5 10 0.029133834566344774 5 11 -0.07815843732745975 5 12 -0.27895587033364677 5 13 -0.3273884923897441 5 14 -0.45910962971534275 5 15 0.007428902280551597 5 16 -0.3026173509145025 5 17 0.08611274293342319 5 18 0.1941193156315677 5 19 -0.12469631092331213 5 20 -0.09358224883871458 5 21 0.4778772544450973 5 22 -0.08423136629509997 5 23 0.18432061166506486 5 24 -0.5581432039829295 5 25 -0.12378946983429984 5 26 -0.1923401737376622 5 27 0.0865249999564881 5 28 0.23767067743040116 5 29 0.298514275239377 5 30 0.4131980415752139 5 31 -0.21817739140373504 5 32 0.03690989806197109 5 33 -0.3368036829133487 5 34 -0.2610021808654892 5 35 0.15924039417953395 5 36 -0.10582824176537997 5 37 -0.1367336692297414 5 38 -0.4730604214553864 5 39 -0.4376152749052906 5 40 -0.4060500663197715 5 41 -0.09036937119922328 5 42 -0.7979623241726657 5 43 -0.6736853258323663 5 44 -0.2692746890105191 5 45 -0.9153715480474212 5 46 -0.5520214219729986 5 47 -0.8504826493596624 5 48 -0.6526731299298305 5 49 -0.8045135728540913 5 50 -0.11140301240273942 5 51 0.5732074618031469 5 52 0.8164963056613956 5 53 0.3548351134792078 5 54 0.8616998328345282 5 55 0.5175378020184276 5 56 0.3752602952645372 6 1 -0.4193394274514972 6 2 -0.4607959677155691 6 3 -0.6159760515889445 6 4 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6 45 1.32381062054651 6 46 1.6724381054277053 6 47 1.5396380142904107 6 48 1.389659700638294 6 49 2.1757361338662022 6 50 1.981734242499222 6 51 2.1943906359274976 6 52 2.4968633242637104 6 53 2.3358641062729077 6 54 2.183369013327237 6 55 2.5353608286649845 6 56 1.8500885171515815 7 1 -0.2575747215691422 7 2 -0.401972438303801 7 3 -0.4600936986477677 7 4 -0.2816821705561665 7 5 -0.34629752155140636 7 6 -0.347932057265794 7 7 -0.15778297059413404 7 8 -0.2161752229833951 7 9 -0.2767837252002465 7 10 -0.3213940689027339 7 11 0.15618042642316965 7 12 -0.13201435868625566 7 13 -0.0448763422305376 7 14 -0.07614499086835025 7 15 0.03244147141077036 7 16 -0.1533463604670402 7 17 -0.30384871173391304 7 18 0.12465392263676733 7 19 -0.07681630421977559 7 20 -0.05077179132237575 7 21 0.08771469369430207 7 22 -0.12306997866312486 7 23 0.27544680573243596 7 24 -0.4831892907937245 7 25 -0.4204879782975155 7 26 -0.4014988799551986 7 27 -0.08824456598954811 7 28 -0.3614662422947532 7 29 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8 14 -0.15217098266041518 8 15 -0.5130441647041408 8 16 0.17842518536059515 8 17 -0.04314419737002595 8 18 -0.007870017171718757 8 19 0.10943540576653987 8 20 0.05015160129048013 8 21 0.702352176594438 8 22 0.6949874768772304 8 23 0.45225939123174896 8 24 0.6002580416276189 8 25 0.5083081913331284 8 26 0.3322904497301664 8 27 0.7131234230665342 8 28 0.2630549615356159 8 29 0.671228447372151 8 30 0.7162690016229121 8 31 0.2661779388818246 8 32 0.19853283584770054 8 33 0.46421334484786314 8 34 0.45813086094197075 8 35 0.8363920538893339 8 36 1.0789594081385516 8 37 0.9109788465060333 8 38 1.1992711229691793 8 39 1.3610662330785388 8 40 1.2962456999294858 8 41 1.1570052395474804 8 42 0.6019084768609275 8 43 0.8795595559008902 8 44 0.6310516211995107 8 45 0.6925178708885085 8 46 0.6539018537177199 8 47 1.0058828843314522 8 48 0.4550837772456795 8 49 1.075770333592608 8 50 0.6831706310081138 8 51 0.7543427563105354 8 52 0.655892052033888 8 53 0.9367190994329597 8 54 0.9004346768019289 8 55 1.1030078874885128 8 56 0.4447875595592432 9 1 -0.178162956863261 9 2 -0.3166783206567416 9 3 -0.7453878162948229 9 4 -0.3463880529091057 9 5 -0.013944580374939619 9 6 -0.6302849984422636 9 7 -0.2842535588294268 9 8 -0.2544105171010429 9 9 -0.22090137225906403 9 10 -0.9949234806674383 9 11 -0.6055842794591868 9 12 -0.5820143586862514 9 13 -0.7889939892893594 9 14 -0.7761449908683531 9 15 -0.5587349991774673 9 16 -0.8356993016435084 9 17 -0.3303192999692044 9 18 -0.2635813714808819 9 19 -0.45622806892566103 9 20 -0.5625364972047251 9 21 -0.6093441298351081 9 22 -0.5671876257219495 9 23 -0.19808260603227268 9 24 -0.47436576138195363 9 25 -0.6057820959445763 9 26 -0.48385182113166536 9 27 -1.567656330695435 9 28 -1.0497015364123996 9 29 -0.37066621747052864 9 30 -0.3045244320296163 9 31 -0.4506141267816446 9 32 -0.504442540349288 9 33 -0.32922030768291677 9 34 -0.3697419160758102 9 35 -1.6645655384499278 9 36 -0.28177930595173706 9 37 0.08912522133503131 9 38 -0.10873161300892775 9 39 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1.0207926149627653 120 42 1.0631487869384486 120 43 0.9805423908953514 120 44 0.9682609235250936 120 45 1.059419864243992 120 46 1.1676061726545974 120 47 1.1396730282960128 120 48 1.0726779079212072 120 49 1.492367786527268 120 50 1.382644606644881 120 51 1.7371777507734372 120 52 1.7388101029751937 120 53 1.9082030418471376 120 54 1.7379208340555294 120 55 1.90360312558375 120 56 1.5269442594485003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 201.0 3 26.0 4 66.0 5 51.0 6 108.0 7 195.0 8 150.0 9 248.0 10 351.0 11 422.0 12 693.0 13 1191.0 14 1552.0 15 1430.0 16 1620.0 17 1935.0 18 2225.0 19 2303.0 20 2805.0 21 2932.0 22 3257.0 23 4374.0 24 5348.0 25 5144.0 26 5911.0 27 6338.0 28 7325.0 29 8709.0 30 10413.0 31 12626.0 32 15137.0 33 19335.0 34 25447.0 35 34365.0 36 47341.0 37 62641.0 38 56795.0 39 29921.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 45.24310176908679 42.196880799934895 3.617190514817644 8.94282691616067 11 25.61330004646511 21.580548708296256 27.92920502663212 24.87694621860652 12 25.33345934040548 15.820975452247257 31.485229608511776 27.360335598835483 13 20.724225647164445 17.749408685562475 34.98953878786447 26.536826879408608 14 19.61168820600056 21.801061084553368 37.290742942947674 21.296507766498397 15 21.49418136092888 25.138935922778664 32.87760775573529 20.48927496055716 16 26.878096033139858 24.52360138712785 27.09939595359789 21.498906626134392 17 26.658108686350023 24.390768931906305 24.292588421525156 24.658533960218517 18 24.900572544634066 24.660371563353994 25.580485704760182 24.85857018725176 19 24.971976552183992 26.338365740777203 26.093702009025254 22.59595569801355 20 24.499187516899386 25.787347314868047 27.09257057052327 22.620894597709295 21 24.819455491939486 23.2774439465415 29.01811614176846 22.88498441975056 22 23.711491763470498 23.39463148913828 28.500623482312793 24.393253265078428 23 24.492656980987434 25.387890975726297 27.428289394233747 22.691162649052522 24 24.42554911807712 27.846396572589644 25.4467367934707 22.281317515862536 25 24.092737700096635 31.18724003193143 22.4068841645309 22.313138103441034 26 25.831890242780826 28.335115845791215 22.869328259961268 22.963665651466698 27 22.028882669084506 26.172060813918346 24.337130918121968 27.46192559887518 28 23.784565240248135 27.523919671958787 24.273998528020186 24.417516559772896 29 25.504266312564916 25.460880083091205 24.429339608998042 24.60551399534584 30 23.799011159322284 25.217542317802554 25.49935401706658 25.484092505808587 31 24.79556143947908 26.645575783735982 25.01901601029628 23.539846766488655 32 27.023932060431676 24.93556955460727 25.800609160574624 22.23988922438643 33 26.396723815543442 24.7960285481025 26.129677912090806 22.677569724263254 34 24.547908409812106 26.795528460637026 28.078265105929134 20.578298023621734 35 23.052219204211045 29.206503096900992 27.700863865448806 20.040413833439157 36 25.393293382230812 29.31396768231755 25.52300703670211 19.76973189874953 37 25.59736676355951 28.45697931517932 25.39260047910567 20.553053442155502 38 24.670991903367884 28.38673945723554 25.67027982171585 21.27198881768072 39 24.74558920142581 26.507270199175224 26.581937822713204 22.16520277668576 40 24.549902178476096 28.559132517141865 25.54527908837204 21.34568621600999 41 23.889500661506652 27.536369662063848 27.05654627509698 21.517583401332523 42 22.69409492813158 27.394535305477106 28.04810072409976 21.86326904229155 43 24.135498462286385 27.530886378288116 26.59002758372877 21.743587575696726 44 24.128720326556493 26.98635384879589 25.59822454721991 23.286701277427706 45 23.228991574429397 26.519404184241964 26.916103728605712 23.33550051272293 46 23.734274236177185 26.932551009620465 25.778623533143037 23.55455122105931 47 23.033455322389575 27.645858013200204 26.41785835166695 22.902828312743274 48 23.455389075825874 27.73765686834698 24.66615876240662 24.14079529342052 49 23.0271750913504 29.195376162701542 24.84281385670546 22.9346348892426 50 20.814825592931125 28.747734758396277 26.519662958504743 23.917776690167862 51 21.95683103172882 28.94362435275637 26.101834760486803 22.997709855028006 52 22.641444530882033 29.895586920052285 26.325538073994103 21.137430475071575 53 22.70018365715919 29.13929892716697 26.6578101927077 21.50270722296614 54 22.79949612033832 28.763748187197645 25.26093768194184 23.175818010522192 55 22.752112855054964 28.018099595433267 25.15859651352439 24.071191035987376 56 21.524898737193233 28.884653059063353 25.20371694067191 24.386731263071507 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 450.5 11 901.0 12 2612.5 13 5325.5 14 6327.0 15 5078.5 16 3830.0 17 3391.5 18 2953.0 19 2652.5 20 2352.0 21 2095.0 22 1874.5 23 1911.0 24 2278.5 25 2646.0 26 3350.5 27 4055.0 28 4425.0 29 5536.0 30 6277.0 31 6444.5 32 6612.0 33 7098.0 34 7584.0 35 8436.0 36 9288.0 37 9173.5 38 9872.5 39 10686.0 40 10830.5 41 10975.0 42 11765.0 43 12555.0 44 13726.5 45 14898.0 46 14816.0 47 14269.0 48 13804.0 49 15148.5 50 16493.0 51 16330.5 52 16168.0 53 18239.5 54 19474.0 55 18637.0 56 19919.0 57 21201.0 58 24330.5 59 27460.0 60 27708.0 61 27956.0 62 25587.0 63 21218.5 64 19219.0 65 15927.0 66 12635.0 67 11386.5 68 10138.0 69 7851.0 70 5564.0 71 4560.5 72 3178.0 73 2799.0 74 2108.5 75 1418.0 76 1117.5 77 817.0 78 608.0 79 325.0 80 251.0 81 202.5 82 154.0 83 118.0 84 82.0 85 56.0 86 30.0 87 21.0 88 8.5 89 5.0 90 3.5 91 2.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0015750884018365528 23 0.006562868340985638 24 5.25029467278851E-4 25 0.03018919436853393 26 0.10028062825026055 27 0.11183127653039526 28 0.12889473421695793 29 0.16433422325828037 30 0.2336381129390887 31 0.25936455683575244 32 0.27931567659234874 33 0.32053048977373855 34 0.3139676214327529 35 0.3564950082823399 36 0.4289490747668213 37 0.3869467173845132 38 0.46228844593902835 39 0.5035032591204182 40 0.5722821193339476 41 0.5906581506887074 42 0.6279352428655058 43 0.6423735532156742 44 0.6394858911456406 45 0.6717752033832899 46 0.674925380186963 47 0.7232280911766172 48 0.76313033068981 49 0.7132525312983191 50 0.7707432579653533 51 0.7321535921203578 52 0.7899068335210313 53 0.801457481801166 54 0.8040826291375603 55 0.8513352811926569 56 0.840309662379801 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 380931.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.388351684942553 #Duplication Level Percentage of deduplicated Percentage of total 1 61.22477282274882 16.156208370774085 2 11.509242814112215 6.074198940123821 3 6.207465874167817 4.9141437767945915 4 4.13951556195068 4.369399698161886 5 3.1106329107423 4.104223760571216 6 2.4067159056554526 3.8105559434508662 7 1.8867772785516892 3.4852259664306553 8 1.4858246082881246 3.1366769844519227 9 1.2018581286410126 2.854354947658737 >10 6.207267273380335 28.28154407235044 >50 0.3669566832040243 6.574673376387325 >100 0.23097273520471284 10.840902360367046 >500 0.016997993499913933 3.321373644030968 >1k 0.004999409852915863 2.0765181584464343 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2472 0.6489364215566599 No Hit AGTCGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1770 0.46465107854178317 No Hit AGTCGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 1337 0.3509821988759119 No Hit AGTCGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 1162 0.30504212048901247 No Hit AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1129 0.2963791342789114 No Hit AGTCGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 975 0.25595186529843983 No Hit AGTCGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 956 0.25096408535929077 No Hit AGTCGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 951 0.24965151169109365 No Hit AGTCGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 942 0.24728887908833883 No Hit AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 880 0.23101296560269444 No Hit AGTCGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 781 0.20502400697239134 No Hit AGTCGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 743 0.19504844709409316 No Hit AGTCGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG 734 0.19268581449133831 No Hit AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 724 0.19006066715494407 No Hit AGTCGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 686 0.18008510727664592 No Hit AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 683 0.17929756307572764 No Hit AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 678 0.1779849894075305 No Hit AGTCGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 640 0.1680094295292323 No Hit AGTCGTGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT 599 0.15724632545001588 No Hit AGTCGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 554 0.14543316243624174 No Hit AGTCGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 554 0.14543316243624174 No Hit AGTCGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG 508 0.13335748468882816 No Hit AGTCGTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 487 0.12784467528240023 No Hit AGTCGTGGGGCAGGAAAGTCACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCT 457 0.11996923327321746 No Hit AGTCGTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC 442 0.11603151226862607 No Hit AGTCGTGGGCACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGA 430 0.11288133546495298 No Hit AGTCGTGGGATTTGGAGGACATGATAGCTTGCATAAAAGATGACGCTATAGTTTAT 412 0.10815607025944331 No Hit AGTCGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 406 0.10658098185760677 No Hit AGTCGTGGGGTCACGGGATCGTGGGTTCGAGTCCCACCTCGTGTTTTGTTGCGAGA 397 0.10421834925485192 No Hit AGTCGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGA 395 0.10369331978757308 No Hit AGTCGTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA 387 0.10159320191845767 No Hit AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 382 0.10028062825026055 No Hit AGTCGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 381 0.10001811351662113 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.002100117869115404 0.0 0.0 0.0 0.0 11 0.0044627504718702336 0.0 0.0 0.0 0.0 12 0.0044627504718702336 0.0 0.0 0.0 0.0 13 0.0044627504718702336 0.0 0.0 0.0 0.0 14 0.006300353607346212 0.0 0.0 0.0 0.0 15 0.006562868340985638 0.0 0.0 0.0 0.0 16 0.0070878978082644884 0.0 0.0 0.0 0.0 17 0.007350412541903915 0.0 0.0 0.0 0.0 18 0.007875442009182766 0.0 0.0 0.0 0.0 19 0.007875442009182766 0.0 0.0 0.0 0.0 20 0.008662986210101042 0.0 0.0 0.0 0.0 21 0.008662986210101042 0.0 0.0 0.0 0.0 22 0.009975559878298169 0.0 0.0 0.0 0.0 23 0.011288133546495297 0.0 0.0 0.0 0.0 24 0.0133882514156107 0.0 0.0 0.0 0.0 25 0.01575088401836553 0.0 0.0 0.0 0.0 26 0.016800942952923233 0.0 0.0 0.0 0.0 27 0.019426090289317487 0.0 0.0 0.0 0.0 28 0.02625147336394255 0.0 0.0 0.0 0.0 29 0.029139135433976233 0.0 0.0 0.0 0.0 30 0.03281434170492819 0.0 0.0 0.0 0.0 31 0.03333937117220704 0.0 0.0 0.0 0.0 32 0.03543948904132244 0.0 0.0 0.0 0.0 33 0.036489547975880145 0.0 0.0 0.0 0.0 34 0.03727709217679842 0.0 0.0 0.0 0.0 35 0.04278990158322636 0.0 0.0 0.0 0.0 36 0.04305241631686579 0.0 0.0 0.0 0.0 37 0.04410247525142349 0.0 0.0 0.0 0.0 38 0.04646510785417832 0.0 0.0 0.0 0.0 39 0.04856522572329372 0.0 0.0 0.0 0.0 40 0.04961528465785142 0.0 0.0 0.0 0.0 41 0.0504028288587697 0.0 0.0 0.0 0.0 42 0.051715402526966825 0.0 0.0 0.0 0.0 43 0.05276546146152453 0.0 0.0 0.0 0.0 44 0.05329049092880338 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATA 15 0.008226696 50.304707 49 CGACTGA 15 0.008226696 50.304707 50 CGCATCG 25 2.011197E-5 50.304703 49 TCGGGTA 15 0.008252589 50.264736 48 GTCCGAT 15 0.008252589 50.264736 48 GTTTCGA 15 0.00825691 50.25808 46 CGATTAT 15 0.00825691 50.25808 46 CCGATTA 15 0.008261234 50.251423 45 CGCTATA 75 0.0 50.251423 44 CGTAACG 15 0.008269885 50.238125 42 CGTAGCG 15 0.008282874 50.21818 41 GGTCGTA 15 0.008300216 50.19162 39 AATTGCG 15 0.008300216 50.19162 39 AACGATT 20 4.1021567E-4 50.191616 39 ACTTCGC 20 4.1101943E-4 50.171715 38 TAACGAT 20 4.1101943E-4 50.171715 38 CTCGCGG 15 0.0083132405 50.171715 38 TTCGTCC 15 0.00834369 50.12533 35 ATCGGTC 20 4.1640986E-4 50.03942 31 TTTTCGA 25 2.0751113E-5 50.03942 31 >>END_MODULE