FastQCFastQC Report
Tue 31 May 2016
SRR212796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212796_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245238
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28221.1507188934830654No Hit
TCTACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19540.7967770084570907No Hit
TCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10400.4240778345933338No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9670.3943108327420709No Hit
TCTACCGGGAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGT7640.31153410156664135No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5960.24302922059387205No Hit
TCTACCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5520.2250874660533849No Hit
TCTACCGGGGTCTAGATTCCTGCGTTTTACAAAGGTCCACAAAGGAGTGTGGGGTCCA4950.201844738580481No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA4030.16433016090491687No Hit
TCTACCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3620.14761170781037194No Hit
TCTACCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3610.14720394066172454No Hit
TCTACCGGGAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCA3310.13497092620230144No Hit
TCTACCGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCACTT3250.1325243233104168No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3190.1300777204185322No Hit
TCTACCGGGAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACA3040.12396121318882065No Hit
TCTACCGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAG2900.11825247310775655No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2730.11132043158075013No Hit
TCTACCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2660.10846606154021807No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2490.10153402001321166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTG203.4322534E-452.0308248
ATAACGT150.00720813752.0095935
TACGTAC307.9590427E-752.0095940
CCATATG307.970266E-751.9989827
ACCCGTA251.6515405E-551.9989830
AACGTAG150.00722560451.97777610
CGGGTCG251.655534E-551.9777766
GGTCGAC251.655534E-551.9777769
CAACGAA150.00722560451.97777614
GGATCGA150.00722560451.9777768
AATATCG150.00722560451.97777611
ATATCGG150.00722560451.97777612
CGGGATA2600.051.9777766
AGTTCGG150.00722560451.97777619
GTATCGC150.00722560451.97777613
TATCGCT150.00722560451.97777614
CGGGTAC401.891749E-951.977776
GGATAGC353.8797225E-851.977778
CTACCGG248000.051.390932
TACCGGG248000.051.390933
Produced by FastQC (version 0.11.5)