FastQCFastQC Report
Tue 31 May 2016
SRR212795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212795_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261892
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29021.1080903578574375No Hit
TCTACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20770.7930750080185724No Hit
TCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11250.4295663861439067No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT10310.39367372810166024No Hit
TCTACCGGGAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGT8900.3398347410382906No Hit
TCTACCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6270.23941166587753732No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6270.23941166587753732No Hit
TCTACCGGGGTCTAGATTCCTGCGTTTTACAAAGGTCCACAAAGGAGTGTGGGGTCCA5410.20657370213675869No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA4140.15808043010095765No Hit
TCTACCGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCACTT3650.1393704275044675No Hit
TCTACCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3630.13860675392910055No Hit
TCTACCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.1298245078123807No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3270.12486062957249554No Hit
TCTACCGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAG3150.12027858812029386No Hit
TCTACCGGGAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACA2940.11226001557894094No Hit
TCTACCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC2910.11111450521589053No Hit
TCTACCGGGAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCA2830.10805981091442274No Hit
TCTACCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT2790.10653246376368884No Hit
TCTACCGGGGCGCTTCCCGACTTCCTCAAGGCCTTCTACGAGTGCCTGGCTGCCTGCG2710.10347776946222106No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2710.10347776946222106No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2650.10118674873612023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA150.00719027352.0432246
CCTAGAC251.6435537E-552.0432242
ATCGTAC150.00720660352.01338227
TTAAGCG150.00720660352.01338227
TGTTCGA150.00720660352.01338225
CGAATTC150.00720660352.01338225
ACGCGAT203.438371E-452.0133824
CGTGATA150.007217504551.99350436
TATTCGA150.007217504551.99350437
AACGCGA251.6547508E-551.98357423
GCACGAA150.0072338851.9637229
CGGGCTA505.456968E-1251.9637226
CGGGTAT900.051.963726
GGAGCAT459.276846E-1151.963728
GGCACGA203.4546273E-451.963728
CGGGGTA2500.051.963726
GTTCGGG203.4546273E-451.9637210
CGGGATT11350.051.505896
TACCGGG264600.051.4236563
ACCGGGT13250.051.375454
Produced by FastQC (version 0.11.5)