FastQCFastQC Report
Tue 31 May 2016
SRR212794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212794_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275100
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27511.0No Hit
TCTACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19900.7233733187931661No Hit
TCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11330.41185023627771716No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9530.346419483824064No Hit
TCTACCGGGAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAAGT8090.2940748818611414No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5840.21228644129407487No Hit
TCTACCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5770.20974191203198836No Hit
TCTACCGGGGTCTAGATTCCTGCGTTTTACAAAGGTCCACAAAGGAGTGTGGGGTCCA5060.18393311523082514No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA4120.14976372228280624No Hit
TCTACCGGGAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCA3570.1297709923664122No Hit
TCTACCGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCACTT3350.12177390039985461No Hit
TCTACCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3340.12141039621955652No Hit
TCTACCGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAG3190.11595783351508542No Hit
TCTACCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3100.11268629589240275No Hit
TCTACCGGGAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACA3060.11123227917121048No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3030.11014176663031626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACG203.357768E-452.264728
GATCTCG251.6029408E-552.264729
CGTTGTA150.007142953652.13083339
CGACCGT203.4067192E-452.1117643
ACGCAGT251.631E-552.11175542
TACGTAC251.6363061E-552.0831840
TTAGGAC150.007179208652.06414450
AATTAGC150.007179208652.06414451
CCTACTC150.00719998752.02611525
AACGCGG150.00719998752.02611523
CGGCCCA203.4376004E-452.01661327
AGTAGCG203.4407008E-452.00711437
TACCCGT150.007220810351.9881435
CGTACAT150.00722602351.97865333
AGCGTAC203.4718265E-451.91235431
CGTCCTA150.007267826251.90289722
CGGAATT150.007294044451.85566321
CTAACCG150.007304551551.83679216
TAACCGG150.007304551551.83679217
ACCGGGT12050.051.8119774
Produced by FastQC (version 0.11.5)