FastQCFastQC Report
Tue 31 May 2016
SRR212793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212793_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464328
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44450.9572974276804327No Hit
TCTACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34770.7488241070967074No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT34210.7367636670629383No Hit
TCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15930.34307644596061404No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC14790.31852483589186953No Hit
TCTACCGGGGTCTAGATTCCTGCGTTTTACAAAGGTCCACAAAGGAGTGTGGGGTC13330.28708154580382833No Hit
TCTACCGGGAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAA12780.27523647077066216No Hit
TCTACCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10820.2330249306524698No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9150.19705897555176516No Hit
TCTACCGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCAC6880.14817112041487912No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA6310.1358953153805069No Hit
TCTACCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGG6270.13503385537809479No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5620.12103513033889836No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5540.11931221033407419No Hit
TCTACCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5520.11888148033286815No Hit
TCTACCGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATG5240.11285126031598354No Hit
TCTACCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA4950.1066056752984959No Hit
TCTACCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4710.10143691528402338No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAT150.008252474550.2666749
TCGAAGT150.00825956450.25575648
ATACGCT204.0816964E-450.24484347
GTACTAT150.00827730750.22847746
ACGGTTG150.00827730750.22847746
TAATACG204.0926432E-450.2175845
CCTACGT150.00828441250.2175844
TCCTACG150.00828441250.2175843
ATGCTCG150.00833070750.14683539
CCGCGAA150.00833070750.14683539
CGTGATA301.0278582E-650.13053536
CGCTATA150.00839153750.05462331
TAGGATA150.00840589750.03297430
TCGCCCG150.00853960249.83362211
TTGGACG150.00853960249.83362212
ATGTCGT150.00853960249.83362210
GTCGTGA150.00853960249.83362212
CGGGATC19100.049.3118026
CCGGGAT66950.049.2381445
TACCGGG470750.049.150823
Produced by FastQC (version 0.11.5)