FastQCFastQC Report
Tue 31 May 2016
SRR212792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441619
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42090.9530839932158716No Hit
TCTACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33240.7526850067592201No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT31030.702641870028237No Hit
TCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15230.34486740833161617No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC13790.3122601156200254No Hit
TCTACCGGGGTCTAGATTCCTGCGTTTTACAAAGGTCCACAAAGGAGTGTGGGGTC11540.2613112207581648No Hit
TCTACCGGGAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGAGCAGGAAA11220.2540651557111447No Hit
TCTACCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9810.22213718159771206No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8890.20130474458752906No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA6760.15307312411830107No Hit
TCTACCGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCAC6410.14514774047312276No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5950.1347315219680313No Hit
TCTACCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5250.11888075467767466No Hit
TCTACCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGG5060.11457840355600642No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4900.11095537103249635No Hit
TCTACCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA4580.10370930598547617No Hit
TCTACCGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGC4420.10008627346196608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAC150.008302850.1890140
CGGTAGG150.00841183350.02363231
CGAACGT204.1971117E-449.96118529
CGACTTA204.2041673E-449.94418325
CCGATAG204.2159477E-449.9158723
CCGACGC150.00849525549.898920
CGGGGTA4850.049.8480536
GATTGCG150.0085295649.8480539
CGGTAGT204.2443274E-449.8480512
ACCGGGT23950.049.1195874
CTACCGG448250.049.108532
TACCGGG448400.049.0921063
CGGGATT22800.049.0828446
TCTACCG448750.049.0538181
ACCGGGG181600.049.0245674
CCGGGAT62250.048.927175
CCGGGGT39650.048.8422935
ACCGGGA200200.048.8271874
CGGGATC17750.048.7247166
ACCGGGC44550.048.4493984
Produced by FastQC (version 0.11.5)