FastQCFastQC Report
Tue 31 May 2016
SRR212791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477032
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33870.7100152610307066No Hit
GCAGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27910.5850760535980815No Hit
GCAGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12660.2653910010229922No Hit
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT12550.26308507605359804No Hit
GCAGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC10740.22514212882993176No Hit
GCAGGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA9370.19642288148384174No Hit
GCAGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8290.17378289087524526No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8260.1731540022472287No Hit
GCAGGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT8060.1689614113937849No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA8050.1687517818511127No Hit
GCAGGAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA7840.16434956145499674No Hit
GCAGGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT7620.15973771151620855No Hit
GCAGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7220.15135252980932098No Hit
GCAGGAGGGGTCCCCAGCAGACCTTTCTCTCTGCTCCTTCTGCCCCCTAAGGCTATAA6990.14653105032786062No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA6760.14170957084640023No Hit
GCAGGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6360.13332438913951267No Hit
GCAGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6260.13122809371279076No Hit
GCAGGAGGGAACTCATGAGAACTTCAACAGATTATATTCTCCAACAACAACGACAATC5810.12179476429254221No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.11215180532962149No Hit
GCAGGAGGGATTGTGACCTTTGTATTCTATTCCGTGTGGTCACAGACACCTGTGCCTA5210.10921699173221083No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA5160.10816884401884988No Hit
GCAGGAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTCCTCCTC4880.10229921682402858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT550.052.05574851
CGGTTGC150.00719898352.03389747
GACTACG150.00719898352.03389747
CGGATGC203.4345512E-452.0338947
CCGCAAT150.0072049652.0229842
TATGCGT150.007213932552.00660732
TAGCCGC150.007213932552.00660739
ACGATAT251.6545362E-551.99570526
TACGGTT150.00721991951.995726
CCAACGG150.00721991951.995730
CGATCGA150.00721991951.995727
TCGGTAT203.4470254E-451.99569730
TACTACG150.00722590951.984822
ACCGGAT251.6576196E-551.9793516
GTCGTAC150.007228905351.97934715
TCGTACA150.007228905351.97934716
GATCGTA251.6586484E-551.9739049
TCGTTTA150.00723190351.973911
TAGTTCG150.00723190351.973913
GATATCG600.051.97399
Produced by FastQC (version 0.11.5)