FastQCFastQC Report
Tue 31 May 2016
SRR212790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477922
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31960.6687283699013646No Hit
GCAGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27150.5680843317528802No Hit
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT12930.2705462397629739No Hit
GCAGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11630.2433451483714916No Hit
GCAGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC9270.19396470553772374No Hit
GCAGGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA8790.18392122563933028No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8130.17011144077903925No Hit
GCAGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8040.16822828829809047No Hit
GCAGGAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA7990.16718209247534116No Hit
GCAGGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT7650.1600679608806458No Hit
GCAGGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT7470.15630165591874826No Hit
GCAGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7060.14772285017220385No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA6990.1462581760203548No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA6800.14228263189390739No Hit
GCAGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6620.13851632693200983No Hit
GCAGGAGGGGTCCCCAGCAGACCTTTCTCTCTGCTCCTTCTGCCCCCTAAGGCTATAA6240.130565238679115No Hit
GCAGGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6100.12763589037541692No Hit
GCAGGAGGGAACTCATGAGAACTTCAACAGATTATATTCTCCAACAACAACGACAATC5620.11759241047702344No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5520.11550001883152482No Hit
GCAGGAGGGATTGTGACCTTTGTATTCTATTCCGTGTGGTCACAGACACCTGTGCCTA5270.11026903971777821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCGA150.00715988852.10560625
CCACGTA150.007162860652.10013626
CGATACT203.413049E-452.10013626
GCGAGAC150.007186676452.05642351
CGACAAT550.052.05642351
CATACGT150.007186676452.05642350
ATAGCGC150.007198606652.03459546
GCGTACG150.0072135452.00733633
GTACGGT150.0072135452.00733635
CGATATT150.007219519551.99643730
CGTTTCG203.4557094E-451.9692218
ACACGTA150.007240477551.95833616
GTCGTAC150.007240477551.95833615
TCGTACA150.007240477551.95833616
ACGTATA150.007240477551.95833613
GATATCG459.458745E-1151.9311569
CAGGAGG486250.051.0393832
AGGAGGG486350.051.028893
GCAGGAG486450.051.01841
AGGGATC18000.050.7771346
Produced by FastQC (version 0.11.5)