FastQCFastQC Report
Tue 31 May 2016
SRR212789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477787
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29080.6086394146345547No Hit
GCAGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23840.49896711296037777No Hit
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT11120.2327396936291695No Hit
GCAGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10430.21829811192016524No Hit
GCAGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC9400.19674038849947778No Hit
GCAGGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA8430.1764384547926168No Hit
GCAGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8030.1680665233671071No Hit
GCAGGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT7870.1647177507969032No Hit
GCAGGAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA7410.15509002965756707No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA7330.15341564337246513No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7270.15215985365863868No Hit
GCAGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6790.14211353594802703No Hit
GCAGGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT6660.1393926582347364No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5990.12536967309700767No Hit
GCAGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5720.11971861938478862No Hit
GCAGGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5590.11699774167149798No Hit
GCAGGAGGGGTCCCCAGCAGACCTTTCTCTCTGCTCCTTCTGCCCCCTAAGGCTATAA5580.11678844338586024No Hit
GCAGGAGGGAACTCATGAGAACTTCAACAGATTATATTCTCCAACAACAACGACAATC5210.10904440681726375No Hit
GCAGGAGGGATTGTGACCTTTGTATTCTATTCCGTGTGGTCACAGACACCTGTGCCTA5120.10716072224652406No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.10192826510558052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC150.00702400152.3586610
GCGTAGA251.643203E-552.05610338
ACCGACC150.007213753652.00692724
CTGTCGT150.00722873751.9796549
ACGTTCA251.6575617E-551.9796549
GTACGGT203.4540688E-451.97419735
ATACTAC150.007231736651.97419448
CGCATGG150.007237738551.96329545
ATCGTTC150.007237738551.96329545
GTAGGAT203.4630223E-451.94695333
GCGTACG251.6637472E-551.9469533
CGGCTCG251.6658134E-551.93605823
GCTCGAT203.4737907E-451.9142911
CGACGTG150.00727985451.8871122
ATAGCGA203.4827844E-451.8871132
GCGACGT150.007282869451.8816821
CTCGATT203.4881898E-451.87081512
CGGTCAG150.007291920551.86538719
CACGACG150.00729795951.85452718
CGTAAGG150.00729795951.85452718
Produced by FastQC (version 0.11.5)