FastQCFastQC Report
Tue 31 May 2016
SRR212788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475903
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30870.6486615970061126No Hit
GCAGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25320.5320411932683762No Hit
GCAGGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT18490.3885245522722067No Hit
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12360.25971679102674283No Hit
GCAGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11890.249840828908412No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9310.1956281006843832No Hit
GCAGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA8680.1823901089087482No Hit
GCAGGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC7540.1584356476004564No Hit
GCAGGAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC7250.15234196884659268No Hit
GCAGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6850.1439368947033324No Hit
GCAGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6620.13910397707095773No Hit
GCAGGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6190.13006852236695293No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT6190.13006852236695293No Hit
GCAGGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC6010.12628623900248578No Hit
GCAGGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG5910.12418497046667074No Hit
GCAGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC5790.12166344822369264No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.1147292620555029No Hit
GCAGGAGGGGTCCCCAGCAGACCTTTCTCTCTGCTCCTTCTGCCCCCTAAGGCTAT5440.11430900834833989No Hit
GCAGGAGGGAACTCATGAGAACTTCAACAGATTATATTCTCCAACAACAACGACAA5140.10800520274089469No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4930.10359253881568303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACAA600.050.23287650
CAACGAC600.050.22756248
CGTTATC150.00827803550.22756248
CCGATCT204.099426E-450.20145
CCGTTCG204.099426E-450.20146
ACTATCC150.00829536650.20099342
ATAGCCG150.00829536650.20099344
ATCCGAT150.00832315150.15854639
TACGGTG150.00833706950.1373536
CGGTATG150.00833706950.1373536
GTACGGT150.00836495950.09500535
ACCGATA150.00836844950.08971834
CGAAACG150.00837543550.07914733
GCGTACG150.00837543550.07914733
CCGTAGT150.00839991850.04218331
CGTGCAA301.0465683E-650.029
TATTCCG550.049.9947328
TACGGTT150.00844904249.9684126
CGGCACG150.0084525649.9631525
CGAATAG150.00845607749.9578924
Produced by FastQC (version 0.11.5)