FastQCFastQC Report
Tue 31 May 2016
SRR212787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422424
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27400.6486373880271954No Hit
GCAGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21820.5165426206844308No Hit
GCAGGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT19800.4687233679904551No Hit
GCAGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10910.2582713103422154No Hit
GCAGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9870.2336514970740299No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC8350.1976686930666818No Hit
GCAGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA7860.18606897335378672No Hit
GCAGGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC6890.16310626290172908No Hit
GCAGGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC6880.16286953392799652No Hit
GCAGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6550.15505747779482226No Hit
GCAGGAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC6440.15245345908376418No Hit
GCAGGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC6130.14511486089805503No Hit
GCAGGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG5750.136119159896218No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT5420.12830710376304377No Hit
GCAGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5390.1275969168418461No Hit
GCAGGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5310.12570308505198569No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.11883794481374166No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4570.10818514099577677No Hit
GCAGGAGGGGTCCCCAGCAGACCTTTCTCTCTGCTCCTTCTGCCCCCTAAGGCTAT4470.10581785125845122No Hit
GCAGGAGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC4400.10416074844232334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGA150.00828755650.21196748
AACGTAT150.00829927850.19401644
CTCGTCA150.00829927850.19401645
ACGTATG150.00829927850.19401645
CGATTAG204.1065336E-450.1820642
ACTATCC150.008307150.18205642
CCGATTA204.1137822E-450.16412441
TAGCGAA150.00836987750.08657533
TCGATGC150.00836987750.08657533
TAGCGAC150.00840534350.03302430
TTGCGAC204.1892828E-449.9795926
ACCACGC150.0084726449.93218623
TCATACC150.00851639749.86715719
GCCGTAA150.00851639749.86715719
ATCTCGT150.00852437149.8553511
ACCGGAT150.00852437149.8553516
GCGGATT150.00852437149.8553514
ACGCGCG252.1212514E-549.8553514
CAGGAGG429700.049.0083772
AGGAGGG429850.048.991283
Produced by FastQC (version 0.11.5)