FastQCFastQC Report
Tue 31 May 2016
SRR212786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93221
Sequences flagged as poor quality0
Sequence length58
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35553.8135184132330697No Hit
TGTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26982.8941976593256884No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16921.8150416751590306No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT8830.947211465227792No Hit
TGTGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6920.7423220089893908No Hit
TGTGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.4837965694425076No Hit
TGTGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4080.4376696237972131No Hit
TGTGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3530.37867004215788286No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCTGA3340.3582883685006597No Hit
TGTGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3210.3443430128404544No Hit
TGTGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3180.34112485384194546No Hit
TGTGCGGGGATGGATTAGGCTAAAGTATATATTTCTGTGCACTTACTTTTTTCAAAAT3100.33254309651258834No Hit
TGTGCGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2450.2628163182115618No Hit
TGTGCGGGGATTTGGAATCAGAATACACGGCTGAGTTTCCAAAGTATTTGAAAGATAA2390.25638000021454393No Hit
TGTGCGGGGATTTCGCTTCTTTCTCACATGCAAAAACAGGAGTGAGATGAAATACTCA2320.24887096255135646No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1950.2091803349030798No Hit
TGTGCGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.1963076989090441No Hit
TGTGCGGGGGAGACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.1823623432488388No Hit
TGTGCGGGGATCGCCCTAGAGTGCTCCTGTGTATAAGAATGAACTAGAGTTGTCATTT1630.1748533055856513No Hit
TGTGCGGGGACCCAAAGGAGAAAAACATCAAACAAGACCAGAGTCGCCCTGAGGGGAT1560.16734426792246382No Hit
TGTGCGGGGAAGGCCTTCTACGAGTGCCTGGCTGCCTGCGAGGGGTCGCGGGAGATCA1530.1641261089239549No Hit
TGTGCGGGGAGACCACTGGGTGTTTAACACAGAGGCGCACCCTCTGCCGGTGTTGCGC1480.1587625105931067No Hit
TGTGCGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1470.15768979092693705No Hit
TGTGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.15661707126076743No Hit
TGTGCGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1350.14481715493290137No Hit
TGTGCGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.13408995827120498No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCAA1210.12979907960652642No Hit
TGTGCGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.12979907960652642No Hit
TGTGCGGGGACCGCTGAAGACCTGCGCTAGGGCTGCTCAGCCCTCAGCCCTCCTCATC1180.12658092060801748No Hit
TGTGCGGGGACCAAAAGCACATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCC1170.12550820094184786No Hit
TGTGCGGGGACTTTTATCTCTTTCCATCAAAGCCCGTCTACCACCATCAGCTGTACCT1140.12229004194333895No Hit
TGTGCGGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.12229004194333895No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.1212173222771693No Hit
TGTGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1120.12014460261099967No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.11799916327866039No Hit
TGTGCGGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.11799916327866039No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1090.11692644361249074No Hit
TGTGCGGGGTCTTTTCCTCTGGCGCGGTGAGTGAGTTCGGTCACGGTTTTTTTACGAT1080.11585372394632111No Hit
TGTGCGGGGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.11370828461398183No Hit
TGTGCGGGGGCCTGTCTCTGCCTCCCGAGTGCTGGGATTAAAGGCATGCGCCATCATA1050.11263556494781218No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA1020.10941740594930327No Hit
TGTGCGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.10941740594930327No Hit
TGTGCGGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.10834468628313364No Hit
TGTGCGGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAG1010.10834468628313364No Hit
TGTGCGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.10405380761845508No Hit
TGTGCGGGGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.10298108795228542No Hit
TGTGCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.10190836828611578No Hit
TGTGCGGGGGACGAGGCTGGGTTCCTGTGTCTGTACTGAGCAGAGAGCCTAGTACACC940.10083564861994615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT251.6412769E-552.01127241
GTCATTT150.00719326752.01127252
ACAACAA251.6412769E-552.01127248
CAGGTGG150.00719326752.01127222
GAAATAC203.4275567E-452.01127249
AGACTAA150.00719326752.01127232
CCTTAAG251.6412769E-552.01127236
TCCATCA150.00719326752.01127223
GCCTTAC150.00719326752.01127228
CCCCTAC150.00719326752.01127236
TACCCGT150.00719326752.01127235
CATATTG203.4275567E-452.01127247
CCCTCCT203.4275567E-452.01127248
CACGGTT251.6412769E-552.01127242
AGATATG150.00719326752.01127239
GGTAAAT150.00719326752.01127243
TTTACGG150.00719326752.01127250
TAAAGGT150.00719326752.01127248
CATGCGC150.00719326752.01127245
ACCATGT150.00719326752.01127239
Produced by FastQC (version 0.11.5)