FastQCFastQC Report
Tue 31 May 2016
SRR212785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97115
Sequences flagged as poor quality0
Sequence length58
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36863.7955001801987334No Hit
TGTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27922.8749420789785303No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16741.7237295989291048No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT8660.8917263038665499No Hit
TGTGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7950.818617103434073No Hit
TGTGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4630.4767543633836174No Hit
TGTGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4170.42938783915975903No Hit
TGTGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3590.36966483035576375No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCTGA3490.3593677598723163No Hit
TGTGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3240.3336250836636977No Hit
TGTGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3240.3336250836636977No Hit
TGTGCGGGGATGGATTAGGCTAAAGTATATATTTCTGTGCACTTACTTTTTTCAAAAT3040.31303094269680276No Hit
TGTGCGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.2883179735365289No Hit
TGTGCGGGGATTTCGCTTCTTTCTCACATGCAAAAACAGGAGTGAGATGAAATACTCA2440.251248519796118No Hit
TGTGCGGGGATTTGGAATCAGAATACACGGCTGAGTTTCCAAAGTATTTGAAAGATAA2090.2152087731040519No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2040.21006023786232816No Hit
TGTGCGGGGGAGACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.17710961231529632No Hit
TGTGCGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.17093137002522782No Hit
TGTGCGGGGACCCAAAGGAGAAAAACATCAAACAAGACCAGAGTCGCCCTGAGGGGAT1620.16681254183184885No Hit
TGTGCGGGGATCGCCCTAGAGTGCTCCTGTGTATAAGAATGAACTAGAGTTGTCATTT1580.16269371363846985No Hit
TGTGCGGGGAAGGCCTTCTACGAGTGCCTGGCTGCCTGCGAGGGGTCGCGGGAGATCA1550.15960459249343562No Hit
TGTGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.15445605725171188No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1470.15136693610667765No Hit
TGTGCGGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.15136693610667765No Hit
TGTGCGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1460.1503372290583329No Hit
TGTGCGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.14415898676826444No Hit
TGTGCGGGGAGACCACTGGGTGTTTAACACAGAGGCGCACCCTCTGCCGGTGTTGCGC1360.14004015857488544No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCAA1350.1390104515265407No Hit
TGTGCGGGGACCGCTGAAGACCTGCGCTAGGGCTGCTCAGCCCTCAGCCCTCCTCATC1310.1348916233331617No Hit
TGTGCGGGGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.13180250218812747No Hit
TGTGCGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1280.13180250218812747No Hit
TGTGCGGGGACTTTTATCTCTTTCCATCAAAGCCCGTCTACCACCATCAGCTGTACCT1230.12665396694640374No Hit
TGTGCGGGGACCAAAAGCACATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCC1220.125624259898059No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.12047572465633527No Hit
TGTGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1150.11841631055964578No Hit
TGTGCGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.11841631055964578No Hit
TGTGCGGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.11841631055964578No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.11120836122123255No Hit
TGTGCGGGGGCCTGTCTCTGCCTCCCGAGTGCTGGGATTAAAGGCATGCGCCATCATA1060.10914894712454307No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA1000.10297070483447458No Hit
TGTGCGGGGATTTAAGCAGTAGTTTTAATTTAAGGAACAAGGTTTGCACAACAAAAAA990.10194099778612985No Hit
TGTGCGGGGAAGACAGGATCCTCATATGAGGACCATGTGCATGGTTGACATTTTGCAG990.10194099778612985No Hit
TGTGCGGGGAGCTTGGCCATGTTGATGGCCTCCGGCATCTTCAACTCCATCTACCCGG980.1009112907377851No Hit
TGTGCGGGGCAGCCCGAGGGTATGTGTGTGGTGAGATCCTGTTTCAAAACAATAAGAT980.1009112907377851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGC307.7677396E-752.14521825
CACGGCT307.7677396E-752.14521826
TCACATG307.7677396E-752.14521824
CTCCCAT150.0071213152.14521827
CACGCCG307.7677396E-752.14521826
TATATAT203.3849693E-452.14521826
AGTATAT203.3849693E-452.14521824
ATATATT203.3849693E-452.14521827
ACAGAAT150.0071213152.14521824
CGCACGC307.7677396E-752.14521824
CCAAATT251.6169528E-552.14521825
TACACGG307.7677396E-752.14521824
TCCATCA150.00713588552.11828623
GCCGGGA150.00713588552.11828623
GCGCACG307.795534E-752.11828623
CCGAGGT150.00713588552.11828622
TTAACAG150.00713588552.11828621
AAGAGGT150.007194404552.01082642
ATACTCA307.907511E-752.01082652
GTAGCGC150.007194404552.01082643
Produced by FastQC (version 0.11.5)