FastQCFastQC Report
Tue 31 May 2016
SRR212784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97876
Sequences flagged as poor quality0
Sequence length58
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32153.2847684825697825No Hit
TGTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24482.5011238710204746No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14331.4640974294004658No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9010.9205525358617026No Hit
TGTGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6520.6661490048632964No Hit
TGTGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.4291143896358658No Hit
TGTGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3620.36985573582900816No Hit
TGTGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3280.3351179042870571No Hit
TGTGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3190.3259225959377171No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCTGA3180.3249008950100127No Hit
TGTGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2820.28811966161265273No Hit
TGTGCGGGGATGGATTAGGCTAAAGTATATATTTCTGTGCACTTACTTTTTTCAAAAT2820.28811966161265273No Hit
TGTGCGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.2860762597572439No Hit
TGTGCGGGGATTTCGCTTCTTTCTCACATGCAAAAACAGGAGTGAGATGAAATACTCA2110.21557889574563732No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1980.2022967836854796No Hit
TGTGCGGGGATTTGGAATCAGAATACACGGCTGAGTTTCCAAAGTATTTGAAAGATAA1960.20025338183007071No Hit
TGTGCGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1950.1992316809023663No Hit
TGTGCGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.15734194286648412No Hit
TGTGCGGGGAAGGCCTTCTACGAGTGCCTGGCTGCCTGCGAGGGGTCGCGGGAGATCA1510.15427684008337078No Hit
TGTGCGGGGGAGACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.1471249335894397No Hit
TGTGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.1471249335894397No Hit
TGTGCGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1400.14303812987862194No Hit
TGTGCGGGGAGACCACTGGGTGTTTAACACAGAGGCGCACCCTCTGCCGGTGTTGCGC1370.1399730270955086No Hit
TGTGCGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1350.13792962524009972No Hit
TGTGCGGGGACCCAAAGGAGAAAAACATCAAACAAGACCAGAGTCGCCCTGAGGGGAT1330.13588622338469084No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCAA1320.13486452245698638No Hit
TGTGCGGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.13486452245698638No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1300.13282112060157752No Hit
TGTGCGGGGACCGCTGAAGACCTGCGCTAGGGCTGCTCAGCCCTCAGCCCTCCTCATC1300.13282112060157752No Hit
TGTGCGGGGATCGCCCTAGAGTGCTCCTGTGTATAAGAATGAACTAGAGTTGTCATTT1260.12873431689075973No Hit
TGTGCGGGGACCAAAAGCACATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCC1230.12566921410764642No Hit
TGTGCGGGGACTTTTATCTCTTTCCATCAAAGCCCGTCTACCACCATCAGCTGTACCT1210.12362581225223754No Hit
TGTGCGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.12056070946912421No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.11545220483060198No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.11238710204748865No Hit
TGTGCGGGGGCCTGTCTCTGCCTCCCGAGTGCTGGGATTAAAGGCATGCGCCATCATA1060.10830029833667087No Hit
TGTGCGGGGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.106256896481262No Hit
TGTGCGGGGAAGACAGGATCCTCATATGAGGACCATGTGCATGGTTGACATTTTGCAG980.10012669091503536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC251.5736692E-552.3849378
ATCTAGG150.006993353452.38493310
GTTTCAT150.006993353452.38493310
GATTTCG353.617606E-852.3849339
GACTTGT150.006993353452.3849339
GTGGACT150.006993353452.3849339
GGAAGGT203.3092155E-452.3849338
GGAACGC150.006993353452.3849338
GATTGCA203.3092155E-452.3849339
GATCTAG203.3092155E-452.3849339
GGATTCT203.3092155E-452.3849338
GACGAGG203.3092155E-452.38493310
AGCTAGT203.3092155E-452.38493310
AAGTGGT150.006993353452.38493310
ATCGCCC307.525887E-752.38493310
ATCGATC150.006993353452.38493310
ATTTCGC353.617606E-852.38493310
TCCCAGT203.4203648E-452.03588527
AGTATAT700.052.0358824
CGCTAGG353.8136932E-852.0358825
Produced by FastQC (version 0.11.5)