FastQCFastQC Report
Tue 31 May 2016
SRR212783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104739
Sequences flagged as poor quality0
Sequence length56
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35763.4142010139489587No Hit
TGTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27282.604569453594172No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15771.5056473710843143No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12391.1829404519806375No Hit
TGTGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9050.8640525496710871No Hit
TGTGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4560.43536791453040413No Hit
TGTGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4000.3819016794126352No Hit
TGTGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3750.35803282444934553No Hit
TGTGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.32461642750073993No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCT3220.30743085192717134No Hit
TGTGCGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3170.3026570809345134No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2980.28451675116241326No Hit
TGTGCGGGGATGGATTAGGCTAAAGTATATATTTCTGTGCACTTACTTTTTTCAAA2950.28165248856681846No Hit
TGTGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.2701954381844394No Hit
TGTGCGGGGACCCAAAGGAGAAAAACATCAAACAAGACCAGAGTCGCCCTGAGGGG2580.24632658322114973No Hit
TGTGCGGGGATTTCGCTTCTTTCTCACATGCAAAAACAGGAGTGAGATGAAATACT2390.22818625344904953No Hit
TGTGCGGGGATTTGGAATCAGAATACACGGCTGAGTTTCCAAAGTATTTGAAAGAT2120.20240789008869664No Hit
TGTGCGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.1919055939048492No Hit
TGTGCGGGGGACGAGGCTGGGTTCCTGTGTCTGTACTGAGCAGAGAGCCTAGTACA1850.1766295267283438No Hit
TGTGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.16421772214743313No Hit
TGTGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT1650.15753444275771203No Hit
TGTGCGGGGATCGCCCTAGAGTGCTCCTGTGTATAAGAATGAACTAGAGTTGTCAT1600.1527606717650541No Hit
TGTGCGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.1527606717650541No Hit
TGTGCGGGGACTTTTATCTCTTTCCATCAAAGCCCGTCTACCACCATCAGCTGTAC1540.14703214657386454No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.14703214657386454No Hit
TGTGCGGGGAAGGCCTTCTACGAGTGCCTGGCTGCCTGCGAGGGGTCGCGGGAGAT1510.1441678839782698No Hit
TGTGCGGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.13748460458854866No Hit
TGTGCGGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.1365298503900171No Hit
TGTGCGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1420.1355750961914855No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC1410.13462034199295392No Hit
TGTGCGGGGACCAAAAGCACATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCC1390.13271083359589075No Hit
TGTGCGGGGGAGACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.1279370626032328No Hit
TGTGCGGGGGCCTGTCTCTGCCTCCCGAGTGCTGGGATTAAAGGCATGCGCCATCA1300.12411804580910643No Hit
TGTGCGGGGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGCCTGTG1270.12125378321351167No Hit
TGTGCGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1240.11838952061791691No Hit
TGTGCGGGGACCTCATTCAGCAGTGATTTAAATAATGTATTATAGTTGTGTAGATG1230.11743476641938531No Hit
TGTGCGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.11648001222085375No Hit
TGTGCGGGGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.1117062412281958No Hit
TGTGCGGGGAGCTTGGCCATGTTGATGGCCTCCGGCATCTTCAACTCCATCTACCC1150.10979673283113263No Hit
TGTGCGGGGCAGCCCGAGGGTATGTGTGTGGTGAGATCCTGTTTCAAAACAATAAG1150.10979673283113263No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.10502296183847468No Hit
TGTGCGGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.10406820763994311No Hit
TGTGCGGGGAGTCCCTTTGTCGTTTGCCATGGATGCCTTAAGTTCTTTGGAGTCTC1090.10406820763994311No Hit
TGTGCGGGGACCGCTGAAGACCTGCGCTAGGGCTGCTCAGCCCTCAGCCCTCCTCA1080.10311345344141151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTA204.057755E-450.26795248
TAGTACA204.057755E-450.26795250
CATGGGC150.00823375350.2679549
AGCCGAT150.00823375350.2679550
AACCTAA150.00823375350.2679550
GCAATGT150.00823375350.2679548
ATTATGG150.00823375350.2679550
TTTTACG150.00823375350.2679550
ATATTGG150.00823375350.2679548
TCACGGT150.00824945950.24373641
GTTGAGC150.00824945950.24373647
GTCATCA150.00824945950.24373644
CTGTACA150.00824945950.24373641
CGCGGGA150.00824945950.24373647
CATGCGC402.5302143E-950.24373645
CGGTTTT150.00824945950.24373644
GGCCGTA204.0674218E-450.24373641
TTCGTCA204.0674218E-450.24373642
AATATTC150.00824945950.24373644
GCACCTA204.0674218E-450.24373646
Produced by FastQC (version 0.11.5)