SRR212782_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212782_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	95868
Sequences flagged as poor quality	0
Sequence length	56
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3204	3.3420953811490803	No Hit
TGTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2445	2.550381774940543	No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1373	1.432177577502399	No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1100	1.147411023490633	No Hit
TGTGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	799	0.8334376434263778	No Hit
TGTGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	399	0.41619727124796596	No Hit
TGTGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	369	0.38490424333458506	No Hit
TGTGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	348	0.3629991237952184	No Hit
TGTGCGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	316	0.3296198940209455	No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCT	310	0.3233612884382693	No Hit
TGTGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	302	0.31501648099470103	No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	294	0.30667167355113284	No Hit
TGTGCGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	257	0.2680769391246297	No Hit
TGTGCGGGGATGGATTAGGCTAAAGTATATATTTCTGTGCACTTACTTTTTTCAAA	255	0.2659907372637376	No Hit
TGTGCGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	222	0.23156840655901867	No Hit
TGTGCGGGGACCCAAAGGAGAAAAACATCAAACAAGACCAGAGTCGCCCTGAGGGG	221	0.23052530562857262	No Hit
TGTGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT	201	0.209663287019652	No Hit
TGTGCGGGGATTTCGCTTCTTTCTCACATGCAAAAACAGGAGTGAGATGAAATACT	200	0.20862018608920602	No Hit
TGTGCGGGGATTTGGAATCAGAATACACGGCTGAGTTTCCAAAGTATTTGAAAGAT	190	0.1981891767847457	No Hit
TGTGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	160	0.1668961488713648	No Hit
TGTGCGGGGGACGAGGCTGGGTTCCTGTGTCTGTACTGAGCAGAGAGCCTAGTACA	158	0.16480994701047275	No Hit
TGTGCGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	153	0.15959444235824258	No Hit
TGTGCGGGGGAGACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	148	0.15437893770601244	No Hit
TGTGCGGGGACTTTTATCTCTTTCCATCAAAGCCCGTCTACCACCATCAGCTGTAC	140	0.1460341302624442	No Hit
TGTGCGGGGAAGGCCTTCTACGAGTGCCTGGCTGCCTGCGAGGGGTCGCGGGAGAT	139	0.14499102933199814	No Hit
TGTGCGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	135	0.14081862561021405	No Hit
TGTGCGGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	133	0.138732423749322	No Hit
TGTGCGGGGATCGCCCTAGAGTGCTCCTGTGTATAAGAATGAACTAGAGTTGTCAT	131	0.13664622188842993	No Hit
TGTGCGGGGGCCTGTCTCTGCCTCCCGAGTGCTGGGATTAAAGGCATGCGCCATCA	130	0.13560312095798388	No Hit
TGTGCGGGGACCAAAAGCACATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCC	130	0.13560312095798388	No Hit
TGTGCGGGGAGACCACTGGGTGTTTAACACAGAGGCGCACCCTCTGCCGGTGTTGC	129	0.13456002002753786	No Hit
TGTGCGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	129	0.13456002002753786	No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	129	0.13456002002753786	No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC	128	0.13351691909709182	No Hit
TGTGCGGGGAGCTTGGCCATGTTGATGGCCTCCGGCATCTTCAACTCCATCTACCC	120	0.1251721116535236	No Hit
TGTGCGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	120	0.1251721116535236	No Hit
TGTGCGGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114	0.11891350607084741	No Hit
TGTGCGGGGACCGCTGAAGACCTGCGCTAGGGCTGCTCAGCCCTCAGCCCTCCTCA	112	0.11682730420995535	No Hit
TGTGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	107	0.1116117995577252	No Hit
TGTGCGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107	0.1116117995577252	No Hit
TGTGCGGGGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107	0.1116117995577252	No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	105	0.10952559769683314	No Hit
TGTGCGGGGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	104	0.10848249676638712	No Hit
TGTGCGGGGTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACTG	102	0.10639629490549506	No Hit
TGTGCGGGGAGTCCCTTTGTCGTTTGCCATGGATGCCTTAAGTTCTTTGGAGTCTC	97	0.1011807902532649	No Hit
TGTGCGGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	96	0.10013768932281887	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACGT	20	4.0892302E-4	50.185104	50
GGCTAAC	20	4.0892302E-4	50.185104	50
ATCTAAT	15	0.008302756	50.158806	48
CACCTAT	15	0.008302756	50.158806	47
GTTGTAA	20	4.099832E-4	50.158806	44
GATGCTG	15	0.008302756	50.158806	46
CCGACCC	15	0.008302756	50.158806	47
GATCTAA	15	0.008302756	50.158806	47
GCTAAGC	15	0.008302756	50.158806	48
TGTGTAG	15	0.008302756	50.158806	47
CGCCCTG	55	0.0	50.158806	45
TAGTTGT	15	0.008319981	50.13253	43
GCGGAAT	15	0.008319981	50.13253	43
AAGTATT	35	5.1346433E-8	50.106285	42
AATCTGA	15	0.008337233	50.10628	42
CGAATGT	15	0.008337233	50.10628	42
AACTAGA	15	0.008337233	50.10628	42
CTTACTT	30	1.0226377E-6	50.10628	42
AACAATC	15	0.008371817	50.05387	39
TACCCAC	15	0.008371817	50.05387	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content