FastQCFastQC Report
Tue 31 May 2016
SRR212781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616661
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA70641.1455240399506375No Hit
CATGATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG28410.4607069362259004No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA27720.4495176442161901No Hit
CATGATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG23910.38773329268431117No Hit
CATGATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT23830.386435983465794No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG20000.3243273046292858No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA18590.30146222965292113No Hit
CATGATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT16990.2755160452825783No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA16560.2685430082330486No Hit
CATGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT16140.26173213483583363No Hit
CATGATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC13350.21648847584004827No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13240.2147046756645872No Hit
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT12300.19946129234701074No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11800.19135310973127861No Hit
CATGATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA11170.18113679963545612No Hit
CATGATGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAG10300.1670285618840822No Hit
CATGATGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAGA9640.15632576083131575No Hit
CATGATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA9610.15583926987437183No Hit
CATGATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA9290.15065003300030325No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9260.15016354204335933No Hit
CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA8700.1410823775137393No Hit
CATGATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC8510.1380012681197611No Hit
CATGATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT7810.1266498124577361No Hit
CATGATGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG6960.11286590201099146No Hit
CATGATGGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTT6840.11091993818321574No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA6670.10816315609386681No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTCCA6460.1047577193952593No Hit
CATGATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTAA6400.10378473748137146No Hit
CATGATGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA6310.10232526461053967No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC6250.10135228269665181No Hit
CATGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG6190.10037930078276396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC353.844616E-852.05977251
AATAACG353.844616E-852.05977249
TAAACGT150.00718814552.05554648
CCGGTAA150.007213570652.00908737
TACGTTG203.4434383E-452.00908738
TACGTAC251.6525868E-552.00908740
TGTCGTA203.4475783E-451.99643332
ATAATCG150.007220516451.9964332
ACGTATG150.007220516451.9964329
TACGCTA150.00722283351.99221428
TCGTACG150.00722283351.99221426
TATAGCG203.448959E-451.99221428
ACGTATC459.276846E-1151.99221424
CAAACGA459.276846E-1151.98799522
TCGACGA150.0072251551.98799521
TACGTAT459.276846E-1151.98799523
GTCGATA251.658946E-551.97535310
GATTCGC251.658946E-551.9753539
CGATAAT251.658946E-551.97535312
AGACCGG150.00723210451.9753514
Produced by FastQC (version 0.11.5)