##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212781_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 616661 Sequences flagged as poor quality 0 Sequence length 58 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.8503310570962 39.0 38.0 40.0 35.0 40.0 2 36.938893492534795 38.0 36.0 39.0 32.0 40.0 3 37.46615887821672 39.0 38.0 40.0 33.0 40.0 4 37.12934659399573 39.0 36.0 39.0 33.0 40.0 5 36.139564525728076 38.0 35.0 39.0 30.0 40.0 6 36.7501382445136 38.0 36.0 39.0 31.0 40.0 7 37.5432936410767 39.0 38.0 40.0 33.0 40.0 8 37.60507312769901 39.0 38.0 40.0 34.0 40.0 9 37.55639808582025 39.0 38.0 40.0 34.0 40.0 10 36.61197481274152 38.0 36.0 39.0 31.0 40.0 11 37.14498403498843 39.0 36.0 39.0 33.0 40.0 12 36.901761259427786 39.0 36.0 39.0 32.0 40.0 13 36.800048973422996 38.0 35.0 39.0 31.0 40.0 14 36.8085122944373 38.0 36.0 39.0 32.0 40.0 15 36.81748480931987 38.0 36.0 39.0 32.0 40.0 16 36.72640235072431 38.0 36.0 39.0 32.0 40.0 17 36.3334571182546 38.0 35.0 39.0 31.0 40.0 18 36.31028555397536 38.0 35.0 39.0 31.0 40.0 19 36.29043996620509 38.0 35.0 39.0 31.0 40.0 20 36.222246907133744 38.0 35.0 39.0 30.0 40.0 21 36.10717233617822 38.0 35.0 39.0 30.0 40.0 22 36.008826567595484 38.0 35.0 39.0 30.0 40.0 23 35.84160340932863 38.0 35.0 39.0 30.0 40.0 24 35.814301536824935 38.0 35.0 39.0 29.0 40.0 25 35.763803775494154 38.0 35.0 39.0 29.0 40.0 26 35.49526887544372 38.0 35.0 39.0 29.0 40.0 27 35.41763951344418 38.0 35.0 39.0 29.0 40.0 28 35.11028587830267 38.0 33.0 39.0 28.0 40.0 29 35.154310066633045 38.0 34.0 39.0 28.0 40.0 30 34.78978887914105 38.0 33.0 39.0 27.0 40.0 31 34.547659086597015 38.0 33.0 39.0 27.0 40.0 32 34.40960592610851 37.0 33.0 38.0 27.0 40.0 33 34.17855677592713 37.0 33.0 38.0 26.0 40.0 34 33.71475737885159 36.0 32.0 38.0 25.0 40.0 35 33.70847029405135 36.0 33.0 38.0 25.0 40.0 36 34.6804921991175 38.0 34.0 39.0 27.0 40.0 37 34.58016803397653 38.0 33.0 39.0 27.0 40.0 38 34.33812094489517 38.0 33.0 39.0 26.0 40.0 39 34.37014177968122 38.0 33.0 39.0 27.0 40.0 40 34.15611981299288 38.0 33.0 39.0 26.0 40.0 41 34.2935648597852 38.0 33.0 39.0 26.0 40.0 42 34.28990482615246 38.0 33.0 39.0 26.0 40.0 43 34.13327095438174 38.0 33.0 39.0 26.0 40.0 44 34.039475497882954 38.0 33.0 39.0 25.0 40.0 45 33.730860229526435 37.0 33.0 39.0 24.0 40.0 46 33.36333901446662 37.0 33.0 39.0 23.0 40.0 47 33.27349224290169 37.0 33.0 39.0 23.0 40.0 48 33.08108669106689 36.0 32.0 39.0 23.0 40.0 49 32.97221001490284 36.0 32.0 39.0 22.0 40.0 50 32.75151501392175 36.0 32.0 39.0 20.0 40.0 51 32.37902996946458 36.0 32.0 39.0 17.0 40.0 52 32.37299748159848 36.0 32.0 39.0 17.0 40.0 53 32.14735973249484 36.0 31.0 39.0 16.0 40.0 54 31.946273560351635 36.0 31.0 39.0 13.0 40.0 55 31.7169579396135 36.0 31.0 39.0 2.0 40.0 56 31.47849304561177 36.0 31.0 39.0 2.0 40.0 57 31.11319509422519 36.0 30.0 39.0 2.0 40.0 58 30.748174442684068 36.0 30.0 38.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -1.7286763850083915 1 2 -2.332607500162595 1 3 -2.6114878847304723 1 4 -2.614371437709984 1 5 -3.461375662069358 1 6 -3.6358196898762785 1 7 -3.155598245832465 1 8 -2.85107255781071 1 9 -2.88842362838858 1 10 -3.191227221021542 1 11 -2.05534860666814 1 12 -2.2433466146685532 1 13 -2.257372451237323 1 14 -2.144036598831171 1 15 -2.3631179249773524 1 16 -2.1772985602994908 1 17 -2.396020894759964 1 18 -1.9867511820004609 1 19 -2.452016545572363 1 20 -2.0230108371926647 1 21 -2.6622171012531695 1 22 -2.896348749445856 1 23 -2.6697146485849643 1 24 -2.8575104091443677 1 25 -2.7605221630102577 1 26 -2.8809846876264587 1 27 -2.7603923006681796 1 28 -2.9584089498979083 1 29 -2.6059845715660614 1 30 -2.29384730488335 1 31 -2.515829315184547 1 32 -2.874915072322956 1 33 -2.322290935893051 1 34 -2.5031630457093037 1 35 -2.655350694506165 1 36 -2.2404508524641784 1 37 -1.874393606349912 1 38 -1.8852407415427024 1 39 -2.179055230263643 1 40 -2.09413982328887 1 41 -2.0440002601627825 1 42 -1.86558115487977 1 43 -2.2450184121868126 1 44 -1.9748808233582977 1 45 -2.3847068618528127 1 46 -2.6926437691502016 1 47 -2.5324066079748704 1 48 -2.580089431061129 1 49 -2.357808371938443 1 50 -2.1001621989278014 1 51 -2.3011168242627456 1 52 -1.8684970188405359 1 53 -2.176478810993366 1 54 -2.100494262109745 1 55 -1.7900673676072074 1 56 -1.7252767046403257 1 57 -1.1944571177961656 1 58 -1.5076142641716856 2 1 -1.2631473701997251 2 2 -1.924940735307068 2 3 -1.6563758155341048 2 4 -1.889657301191761 2 5 -2.5037597768967643 2 6 -2.442336306109574 2 7 -1.825616723191004 2 8 -1.9198136873396692 2 9 -1.8898964613157645 2 10 -1.999625046584569 2 11 -1.4481799271298001 2 12 -1.732166473812164 2 13 -1.7407763020259566 2 14 -1.2503416626985597 2 15 -1.8552985210730029 2 16 -1.0549463122208635 2 17 -1.6242497462180694 2 18 -1.6669121224712384 2 19 -1.8088437956593921 2 20 -1.3794698790479032 2 21 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-1.2807085676697696 117 21 -1.2498825399214795 117 22 -1.1006772812406211 117 23 -1.3920180876301131 117 24 -1.26299676796679 117 25 -1.1685424867080272 117 26 -1.3762123871112237 117 27 -1.4640093730940933 117 28 -1.4712509539392542 117 29 -1.4299909649543636 117 30 -1.4216890815175915 117 31 -1.8968661616327722 117 32 -1.5950678898969812 117 33 -1.9383757652402664 117 34 -2.415591818555594 117 35 -1.7769355271020508 117 36 -1.5482366219773311 117 37 -1.1618686727852676 117 38 -1.2282356281315288 117 39 -1.497854000965809 117 40 -1.6059942307634145 117 41 -1.3570878638766644 117 42 -1.0615346078942167 117 43 -1.0268830204943171 117 44 -1.0268920767612855 117 45 -1.5122432632328469 117 46 -1.3702454688558738 117 47 -1.246432428427216 117 48 -1.2877088685209266 117 49 -1.3890086266344 117 50 -1.4159007197420515 117 51 -1.5490880280260093 117 52 -1.6164389845475426 117 53 -1.0484681033674335 117 54 -1.6738460479052506 117 55 -1.6067902363153408 117 56 -1.3797478531761485 117 57 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118 37 -0.7021026451620997 118 38 -1.3919286811102936 118 39 -0.9825066499041739 118 40 -0.9595605057582617 118 41 -0.8711622815225084 118 42 -1.0294845151298375 118 43 -0.8996875968703222 118 44 -0.6817055172890107 118 45 -1.43023955265771 118 46 -1.0841910470871667 118 47 -1.151220101072031 118 48 -0.9134509835487563 118 49 -1.0493621619879328 118 50 -0.9540629543328549 118 51 -0.9271131774797325 118 52 -0.9946496627582206 118 53 -0.9509057450907896 118 54 -1.0394562313725757 118 55 -1.0705245179067653 118 56 -1.1071339591336837 118 57 -1.2354899388433118 118 58 -1.431122990402475 119 1 -1.294543065599214 119 2 -1.9092828041551257 119 3 -1.8451917310600408 119 4 -1.8020537842777884 119 5 -2.357244757630376 119 6 -2.3395485344109375 119 7 -1.7343621727045573 119 8 -1.6024611656782923 119 9 -1.8158113485914313 119 10 -1.9323097803678024 119 11 -1.0947322018408059 119 12 -1.4686959694798887 119 13 -1.6082480411101088 119 14 -1.68579926600259 119 15 -1.6746947699819543 119 16 -1.6680857753166833 119 17 -1.826786309775855 119 18 -1.899348841437451 119 19 -1.9366619039343576 119 20 -2.2177227051394865 119 21 -1.6290993747191749 119 22 -2.6231438229066413 119 23 -2.1239640171028924 119 24 -2.0536516783743792 119 25 -2.03402732908841 119 26 -1.624462636337718 119 27 -1.9704686006727528 119 28 -2.093020694485439 119 29 -2.119622626274925 119 30 -1.6755953516946107 119 31 -1.9127133509261043 119 32 -1.9359942376570416 119 33 -1.7556475486688967 119 34 -2.3214332743482373 119 35 -2.370110078623494 119 36 -1.9787398787090282 119 37 -1.8438866584153644 119 38 -1.8274381033509215 119 39 -1.9226702448513677 119 40 -1.6700906362200527 119 41 -1.4991960359230063 119 42 -1.8628920529223407 119 43 -1.610181487945077 119 44 -1.7016889507120823 119 45 -1.2749693103715245 119 46 -1.3230293158798752 119 47 -1.4952095398792373 119 48 -1.7743901013785326 119 49 -1.8197856451107342 119 50 -1.4513967708580182 119 51 -1.3364732934457706 119 52 -1.9007792962227086 119 53 -1.6934456084165284 119 54 -1.6299666890242648 119 55 -1.8826129420111322 119 56 -2.013232530266425 119 57 -2.283993769864228 119 58 -2.2735644896776854 120 1 -2.167226683157544 120 2 -2.710366347835482 120 3 -2.544836706331445 120 4 -2.4893036765390733 120 5 -3.8129077098764768 120 6 -3.5618460027220706 120 7 -2.5285299167584085 120 8 -3.2089720045353687 120 9 -3.460663490026718 120 10 -3.0925497469859025 120 11 -2.4060115905689443 120 12 -2.391199338452509 120 13 -2.571268008685884 120 14 -2.5307786417336757 120 15 -2.1889265416169152 120 16 -2.426504786583081 120 17 -2.4149165812774243 120 18 -2.330091657146099 120 19 -2.7472401359248906 120 20 -2.286285573246772 120 21 -2.618393908432843 120 22 -2.2976497639273887 120 23 -2.288445126914297 120 24 -2.810340029595764 120 25 -2.277202166796279 120 26 -2.5624598590729804 120 27 -2.2962089195793567 120 28 -2.4012013124610405 120 29 -2.605390854639964 120 30 -2.4189925073924456 120 31 -2.31330300664105 120 32 -2.390863685692775 120 33 -2.224396479832407 120 34 -2.979788668466732 120 35 -2.1396860327096974 120 36 -1.570789787387639 120 37 -1.5820439501034045 120 38 -1.752841867023477 120 39 -2.034472251869772 120 40 -2.006803203000956 120 41 -2.19437412973436 120 42 -2.0254372860826066 120 43 -1.743620165802895 120 44 -2.251320586904079 120 45 -2.0443263667445244 120 46 -2.0059628188589365 120 47 -1.4221387469906759 120 48 -2.0812983763961483 120 49 -1.983603221228993 120 50 -1.7716646469345498 120 51 -1.9117159620825142 120 52 -1.8223591904677505 120 53 -1.432362201547246 120 54 -1.796356313272657 120 55 -1.9961387685210141 120 56 -1.6031345051342285 120 57 -2.0534192317726045 120 58 -1.6353408446203304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 505.0 3 45.0 4 112.0 5 109.0 6 180.0 7 291.0 8 331.0 9 493.0 10 589.0 11 670.0 12 971.0 13 1255.0 14 1316.0 15 1538.0 16 1830.0 17 1803.0 18 2012.0 19 2186.0 20 2522.0 21 2755.0 22 3314.0 23 3853.0 24 4628.0 25 5761.0 26 7171.0 27 8869.0 28 10744.0 29 13723.0 30 16705.0 31 19922.0 32 24976.0 33 30837.0 34 39209.0 35 52751.0 36 72394.0 37 100041.0 38 99437.0 39 80693.0 40 120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 49.041045242037356 40.56783224494495 3.2745706311895844 7.116551881828103 11 34.92761825378936 20.62737873807489 23.74011004425446 20.70489296388129 12 33.31311693134478 14.858568970633785 27.141168324249463 24.687145773771977 13 24.09638358838973 15.699549671537522 30.366116877830766 29.837949862241974 14 20.911813784234774 19.39898907179147 35.95362768198411 23.735569461989652 15 27.74879552947243 20.66743316019661 30.22535882762166 21.3584124827093 16 33.783338927997484 21.28031862017744 24.55911614036283 20.377226311462252 17 29.36716948454411 21.486464134090532 25.648576682699325 23.497789698666036 18 30.595465896928616 22.391593422631594 24.48739986378231 22.52554081665748 19 26.77473340079396 27.132631483355386 23.18294154277263 22.909693573078023 20 29.829855964609003 24.63310057179553 23.909193810467457 21.62784965312801 21 27.25204333160353 25.25201089776855 26.561689154125585 20.934256616502335 22 25.800482924922854 20.661013623412614 31.099846106194835 22.4386573454697 23 29.580552830234573 21.53848648736307 27.464424425723067 21.41653625667929 24 32.45867664729892 21.555927811228536 23.97054459419357 22.014850947278973 25 27.445962841700194 23.41164241501381 22.432981599230693 26.709413144055304 26 25.723261973539728 24.1258168477018 26.37868645861548 23.77223472014299 27 23.094662378795473 21.05597747732407 25.975190675664063 29.8741694682164 28 22.538845816978622 26.777175852337237 24.93495961332598 25.749018717358158 29 29.09102704456068 22.48355836678425 24.186838584113854 24.238576004541216 30 25.931490092118796 20.793733715349997 28.707546343315304 24.567229849215902 31 30.18614173637113 21.16522715326762 25.303692984562275 23.34493812579898 32 33.78853096587315 20.313275444304246 25.64687259764313 20.251320992179465 33 31.944120089262544 19.94362706938606 27.289298873838806 20.822953967512586 34 25.705578289549447 22.58661489479378 31.534779766799502 20.17302704885727 35 24.117130896404294 29.323597295232595 27.954020900475395 18.605250907887715 36 23.814382982727377 32.205564811675366 25.814840471180663 18.165211734416598 37 29.013600530847395 27.122537813953564 24.463758146447038 19.400103508752007 38 25.207711281318968 29.642833960794494 23.898318836816827 21.251135921069714 39 29.48026769417968 25.23496248225512 23.763455688501317 21.521314135063882 40 25.178151893147806 24.986046390882777 24.11394582105632 25.721855894913098 41 21.90046306061324 28.196562253583284 25.748863440926513 24.154111244876965 42 21.718511882446307 24.96587742489382 25.02154469367584 28.29406599898403 43 22.594068243599626 21.33484805452996 27.4225666409705 28.64851706089991 44 23.9760379161684 21.256451735904346 25.828686028229438 28.938824319697815 45 26.74413510790363 23.469303319450237 25.784108369284986 24.00245320336115 46 23.295735749185006 27.356988818085785 26.58296863514441 22.764306797584798 47 23.11268760298379 22.911387267753796 27.440644810428655 26.53528031883376 48 23.993572106839377 22.206747664613314 29.403066234894045 24.39661399365327 49 26.04263236767292 23.013328094532216 27.733606963275896 23.21043257451897 50 21.238301520396433 22.95402440687653 28.810083586083273 26.997590486643762 51 20.512025505499786 23.386101840188044 31.19196654675792 24.90990610755425 52 20.833948734761716 24.301242110012673 28.27248839122976 26.592320763995854 53 21.23081269422733 23.301271955603326 32.21470298846245 23.253212361706897 54 21.266235084181318 23.026013480051894 31.05591574886381 24.65183568690298 55 21.01905448686715 23.646436297725547 26.81303475023875 28.521474465168556 56 21.954876469909795 25.38584370669868 24.762223460401984 27.89705636298954 57 26.291194199310976 26.46044593446612 24.46093647212463 22.78742339409828 58 24.917559300448023 30.076478978027726 24.229280987144126 20.776680734380125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 248.5 9 1337.0 10 2177.0 11 2682.5 12 3188.0 13 2755.0 14 2322.0 15 2047.0 16 1799.5 17 1827.0 18 1763.5 19 1700.0 20 1948.5 21 2197.0 22 2533.0 23 3446.0 24 4023.0 25 5002.5 26 5982.0 27 6880.5 28 8021.0 29 8263.0 30 9503.5 31 10744.0 32 11490.5 33 12237.0 34 12035.0 35 12041.0 36 12249.0 37 12197.0 38 12145.0 39 13895.5 40 15646.0 41 16473.0 42 18504.0 43 19708.0 44 20304.5 45 20901.0 46 22555.0 47 24373.5 48 24538.0 49 25467.0 50 26396.0 51 27505.0 52 28614.0 53 32283.0 54 37716.0 55 39480.0 56 45484.5 57 51489.0 58 55905.5 59 54348.0 60 48374.0 61 40942.0 62 33510.0 63 27204.0 64 20898.0 65 16958.0 66 11839.5 67 10661.0 68 9071.0 69 7481.0 70 6576.0 71 5671.0 72 4627.0 73 2796.5 74 2010.0 75 1682.5 76 1355.0 77 1096.0 78 645.0 79 453.0 80 348.0 81 243.0 82 179.0 83 115.0 84 85.0 85 37.0 86 19.0 87 14.5 88 10.0 89 7.5 90 5.0 91 4.5 92 2.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 3.243273046292858E-4 17 4.864909569439287E-4 18 1.621636523146429E-4 19 8.108182615732145E-4 20 0.0011351455662025002 21 0.003405436698607501 22 3.243273046292858E-4 23 0.0025946184370342863 24 0.0 25 3.243273046292858E-4 26 0.01848665636386929 27 0.00616221878795643 28 0.01962180193007179 29 0.013946074099059288 30 0.045892313605043936 31 0.04037874942634608 32 0.012810928532856787 33 0.008594673572676074 34 0.017838001754610717 35 0.01945963827775715 36 0.04086524038329001 37 0.046703131866617155 38 0.06827089762446466 39 0.04637880456198787 40 0.05400049622077608 41 0.05302751430688822 42 0.08108182615732144 43 0.07881153502491645 44 0.05740593291938358 45 0.0525410233499443 46 0.06454113362122788 47 0.10800099244155216 48 0.09664953677952716 49 0.1206497573220943 50 0.1247038486299604 51 0.10329824652442751 52 0.08627106303139002 53 0.12308221210681396 54 0.1277849580239386 55 0.15454196065585468 56 0.17221779875815074 57 0.1637852888377893 58 0.17302861701972397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 616661.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.452895580513697 #Duplication Level Percentage of deduplicated Percentage of total 1 59.606295017881905 13.383339179777964 2 11.343279100173973 5.093789223536591 3 6.203346571001426 4.178492784252982 4 4.061134498389807 3.6473691532307284 5 3.0257180469508813 3.396806568213199 6 2.4049782599053193 3.2399235445835797 7 1.997307869560866 3.139174152617188 8 1.606651266047461 2.885917848869102 9 1.3252211878124671 2.6779547655933893 >10 7.642695058713948 29.5023319989417 >50 0.4362540429087057 6.77038001495306 >100 0.31523340309848336 13.625206730885711 >500 0.020290885716683985 3.0093289684344358 >1k 0.010870117348223563 4.300598754511521 >5k 7.246744898815709E-4 1.1493863115988565 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 7064 1.1455240399506375 No Hit CATGATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 2841 0.4607069362259004 No Hit CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 2772 0.4495176442161901 No Hit CATGATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 2391 0.38773329268431117 No Hit CATGATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 2383 0.386435983465794 No Hit CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 2000 0.3243273046292858 No Hit CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA 1859 0.30146222965292113 No Hit CATGATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT 1699 0.2755160452825783 No Hit CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 1656 0.2685430082330486 No Hit CATGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1614 0.26173213483583363 No Hit CATGATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 1335 0.21648847584004827 No Hit CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1324 0.2147046756645872 No Hit CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 1230 0.19946129234701074 No Hit CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1180 0.19135310973127861 No Hit CATGATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA 1117 0.18113679963545612 No Hit CATGATGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAG 1030 0.1670285618840822 No Hit CATGATGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAGA 964 0.15632576083131575 No Hit CATGATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 961 0.15583926987437183 No Hit CATGATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA 929 0.15065003300030325 No Hit CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 926 0.15016354204335933 No Hit CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 870 0.1410823775137393 No Hit CATGATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 851 0.1380012681197611 No Hit CATGATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT 781 0.1266498124577361 No Hit CATGATGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG 696 0.11286590201099146 No Hit CATGATGGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTT 684 0.11091993818321574 No Hit CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 667 0.10816315609386681 No Hit CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTCCA 646 0.1047577193952593 No Hit CATGATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTAA 640 0.10378473748137146 No Hit CATGATGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA 631 0.10232526461053967 No Hit CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 625 0.10135228269665181 No Hit CATGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG 619 0.10037930078276396 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 6.486546092585716E-4 0.0 0.0 0.0 0.0 11 0.0011351455662025002 0.0 0.0 0.0 0.0 12 0.0011351455662025002 0.0 0.0 0.0 0.0 13 0.0011351455662025002 0.0 0.0 0.0 0.0 14 0.0021081274800903577 0.0 0.0 0.0 0.0 15 0.0021081274800903577 0.0 0.0 0.0 0.0 16 0.0024324547847196436 0.0 0.0 0.0 0.0 17 0.0024324547847196436 0.0 0.0 0.0 0.0 18 0.0025946184370342863 0.0 0.0 0.0 0.0 19 0.002756782089348929 0.0 0.0 0.0 0.0 20 0.0038919276555514294 0.0 0.0 0.0 0.0 21 0.0038919276555514294 0.0 0.0 0.0 0.0 22 0.004702745917124644 0.0 0.0 0.0 0.0 23 0.005513564178697858 0.0 0.0 0.0 0.0 24 0.007135200701844287 0.0 0.0 0.0 0.0 25 0.00729736435415893 0.0 0.0 0.0 0.0 26 0.007783855311102859 0.0 0.0 0.0 0.0 27 0.00843250992036143 0.0 0.0 0.0 0.0 28 0.01232443757591286 0.0 0.0 0.0 0.0 29 0.013621746794430003 0.0 0.0 0.0 0.0 30 0.01459472870831786 0.0 0.0 0.0 0.0 31 0.015892037926835002 0.0 0.0 0.0 0.0 32 0.016378528883778932 0.0 0.0 0.0 0.0 33 0.01686501984072286 0.0 0.0 0.0 0.0 34 0.01735151079766679 0.0 0.0 0.0 0.0 35 0.027243493588860006 0.0 0.0 0.0 0.0 36 0.02870296645969179 0.0 0.0 0.0 0.0 37 0.02967594837357965 0.0 0.0 0.0 0.0 38 0.030486766635152863 0.0 0.0 0.0 0.0 39 0.031621912201355365 0.0 0.0 0.0 0.0 40 0.03308138507218715 0.0 0.0 0.0 0.0 41 0.03502734889996287 0.0 0.0 0.0 0.0 42 0.03600033081385072 0.0 0.0 0.0 0.0 43 0.037459803684682505 0.0 0.0 0.0 0.0 44 0.038270621946255724 0.0 0.0 0.0 0.0 45 0.039243603860143576 0.0 0.0 0.0 0.0 46 0.040216585774031435 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCC 35 3.844616E-8 52.059772 51 AATAACG 35 3.844616E-8 52.059772 49 TAAACGT 15 0.007188145 52.055546 48 CCGGTAA 15 0.0072135706 52.009087 37 TACGTTG 20 3.4434383E-4 52.009087 38 TACGTAC 25 1.6525868E-5 52.009087 40 TGTCGTA 20 3.4475783E-4 51.996433 32 ATAATCG 15 0.0072205164 51.99643 32 ACGTATG 15 0.0072205164 51.99643 29 TACGCTA 15 0.007222833 51.992214 28 TCGTACG 15 0.007222833 51.992214 26 TATAGCG 20 3.448959E-4 51.992214 28 ACGTATC 45 9.276846E-11 51.992214 24 CAAACGA 45 9.276846E-11 51.987995 22 TCGACGA 15 0.00722515 51.987995 21 TACGTAT 45 9.276846E-11 51.987995 23 GTCGATA 25 1.658946E-5 51.975353 10 GATTCGC 25 1.658946E-5 51.975353 9 CGATAAT 25 1.658946E-5 51.975353 12 AGACCGG 15 0.007232104 51.97535 14 >>END_MODULE