FastQCFastQC Report
Tue 31 May 2016
SRR212779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628002
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA65841.0484043044448903No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA25400.4044573106455075No Hit
CATGATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG24690.39315161416683386No Hit
CATGATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT23030.3667185773293715No Hit
CATGATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG21240.33821548339018026No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG18420.29331116779882865No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA17340.276113770338311No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA15710.2501584389858631No Hit
CATGATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT15550.24761067639911977No Hit
CATGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT15520.24713297091410535No Hit
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT12940.2060502992028688No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11410.18168731946713546No Hit
CATGATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC11090.17659179429364874No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10760.1713370339584906No Hit
CATGATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA10450.1664007439466753No Hit
CATGATGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAG9270.1476109948694431No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8640.13757917968414113No Hit
CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA8360.13312059515734026No Hit
CATGATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA8320.13248365451065444No Hit
CATGATGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAGA8210.13073206773226836No Hit
CATGATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA7730.12308877997203829No Hit
CATGATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT7400.11783401963688014No Hit
CATGATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7300.11624166802016553No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA6970.11098690768500737No Hit
CATGATGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG6630.10557291218817776No Hit
CATGATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTAA6490.1033436199247773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATCG503.6379788E-1252.3399249
CTACGTA251.6431562E-552.0595639
GAATCGT150.007201921752.03044524
TAACGTC150.007201921752.03044525
ATATCGT203.436509E-452.0304424
TATCGTA203.436509E-452.0304425
TCGTTAC150.00722242451.99305350
CTATCGG150.007224704651.98890337
CGGAAAT150.00722698651.98475343
TACGTAC251.657202E-551.9847540
AATAACG401.906301E-951.9598649
TATATCG150.007247541651.94742623
ACGTAGT150.00727501651.89774731
CGTTGCA150.00727960351.88947316
TCGAATG150.007297971351.85641518
ATGGGAT85700.051.6986545
GATGGGA256550.051.411144
ATGGGAG78200.051.068245
CATGATG639200.050.935621
ATGATGG639600.050.9037672
Produced by FastQC (version 0.11.5)