FastQCFastQC Report
Tue 31 May 2016
SRR212778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212778_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677905
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC107541.5863579705120923No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC37800.5576002537228668No Hit
CATGATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA24870.3668655637589338No Hit
CATGATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG24460.36081751867887096No Hit
CATGATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG22680.33456015223372004No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT20890.3081552724939335No Hit
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT20130.2969442621016219No Hit
CATGATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT19510.28779843783420983No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC18200.26847419623693586No Hit
CATGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC17930.2644913372817725No Hit
CATGATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC15890.234398625176094No Hit
CATGATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT14770.21787713617689794No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT14250.21020644485584264No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13330.19663522174936016No Hit
CATGATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA11410.1683126691793098No Hit
CATGATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCA11070.1632972171616967No Hit
CATGATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA10820.15960938479580472No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9810.14471054203760114No Hit
CATGATGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGC9610.14176027614488756No Hit
CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9390.13851498366290263No Hit
CATGATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC8510.12553381373496286No Hit
CATGATGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCA8070.11904322877099298No Hit
CATGATGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC7770.11461782993192261No Hit
CATGATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA7710.11373275016410855No Hit
CATGATGGGGTTTTGAGACAAGGTCTCTCAGTGTAGCTGCAGCTCTCCTGAAATTA7610.11225761721775177No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC7410.10930735132503816No Hit
CATGATGGGATCCCCAAACCTGTCCAGCACTTATGTAAGAACGCATTTGGAGGACA6970.10281676636106829No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTC6930.10222671318252556No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC6800.10030904035226175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG550.050.1678138
CGTTAGT507.2759576E-1250.08595731
GTACCGT150.00842165550.01177627
ACGACAG301.0546446E-649.94889525
ACGTATC507.2759576E-1249.9230524
TACGTAT507.2759576E-1249.88617323
CGTATTC204.260883E-449.81258417
GATATCG402.7703209E-949.8125849
AATTCGT150.00855554749.81258413
TCCGTCG150.00855554749.81258415
CGTCGTG150.00855554749.81258417
ATGGGAC49750.048.911455
CATGATG691700.048.8187831
ATGATGG692000.048.797612
TGATGGG692850.048.7377473
GATGGGA288300.048.7327044
ATGGGAT102100.048.6904565
GATGGGG332600.048.412264
ATGGGAG86700.048.404965
ATGGGGG131350.048.0301785
Produced by FastQC (version 0.11.5)