FastQCFastQC Report
Tue 31 May 2016
SRR212777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615284
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC97801.5895098848661755No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC33660.5470644450367634No Hit
CATGATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA23990.38990124885418764No Hit
CATGATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG23380.3799871278954109No Hit
CATGATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG21100.3429310692298191No Hit
CATGATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT20630.3352923202943681No Hit
CATGATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC20500.333179474844137No Hit
CATGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC18680.30359963854090144No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT18310.2975861553363975No Hit
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT18260.296773522470924No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC16730.27190695678743476No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT14370.2335506855370853No Hit
CATGATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT14190.2306252072213807No Hit
CATGATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA11890.19324409540959947No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11510.18706808563200084No Hit
CATGATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA10410.1691901625915837No Hit
CATGATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCA10270.1669147905682579No Hit
CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9090.1477366549430832No Hit
CATGATGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGC8980.1459488626390415No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8700.14139811859238985No Hit
CATGATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC7930.12888357246409787No Hit
CATGATGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC7810.12693325358696148No Hit
CATGATGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCA7460.12124482352864695No Hit
CATGATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA7240.11766923892056352No Hit
CATGATGGGGTTTTGAGACAAGGTCTCTCAGTGTAGCTGCAGCTCTCCTGAAATTA7150.11620649976271119No Hit
CATGATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCT6960.11311849487391189No Hit
CATGATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA6800.11051806970439668No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTC6580.10694248509631324No Hit
CATGATGGGAGAGTCCTGTGCTGTAAGACAGCTTGACACAACTCGATGTCATTCTT6290.10222921447656692No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC6170.10027889559943051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT600.050.17175341
AACGACC150.008331791550.14714440
GTACGAT204.122151E-450.1471440
CGGCAAT150.0083452650.12665636
ATTCGTT355.1797542E-850.12665636
TATCCGT451.364242E-1050.00407427
AGCGTAC150.0084372849.98777826
ACTCATC150.0084372849.98777826
CGTATCC451.364242E-1049.9755625
ACGTATC451.364242E-1049.9674224
ATACGCC150.00845087549.9674224
ATATGCG150.00848357149.9186322
CGATTCA150.008489029549.91050721
TCGCAAC150.008527311549.85371816
GTCGATA150.008527311549.85371810
GGTCGAT150.008527311549.8537189
GTTACGG850.049.85371812
CTAACGG252.1228032E-549.85371818
ACGTGTA150.008527311549.85371814
CGTCACT150.008527311549.85371814
Produced by FastQC (version 0.11.5)