##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212777_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 615284 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.35346279116636 39.0 38.0 40.0 35.0 40.0 2 37.83225144811177 39.0 38.0 40.0 33.0 40.0 3 38.17283888415756 39.0 38.0 40.0 35.0 40.0 4 37.964510697499044 39.0 38.0 40.0 35.0 40.0 5 37.38272407538633 39.0 37.0 40.0 33.0 40.0 6 37.798676708641864 39.0 38.0 40.0 33.0 40.0 7 38.46837720467296 39.0 38.0 40.0 36.0 40.0 8 38.41477756613206 39.0 38.0 40.0 36.0 40.0 9 38.39546940924841 39.0 38.0 40.0 36.0 40.0 10 37.45891978338458 39.0 37.0 40.0 33.0 40.0 11 37.60891068189649 39.0 38.0 40.0 33.0 40.0 12 37.54132725700652 39.0 38.0 40.0 33.0 40.0 13 37.56070042451941 39.0 38.0 40.0 33.0 40.0 14 37.582971440830576 39.0 38.0 40.0 33.0 40.0 15 37.575628490258154 39.0 38.0 40.0 33.0 40.0 16 37.50170977954895 39.0 38.0 40.0 33.0 40.0 17 37.17458767008406 39.0 36.0 40.0 33.0 40.0 18 37.14119658564175 39.0 36.0 40.0 33.0 40.0 19 37.154725297586154 39.0 36.0 40.0 33.0 40.0 20 37.03752576046183 39.0 36.0 40.0 33.0 40.0 21 36.82608844046001 39.0 36.0 40.0 32.0 40.0 22 36.79101845651764 38.0 36.0 40.0 32.0 40.0 23 36.52938968021272 38.0 36.0 40.0 31.0 40.0 24 36.47503266784119 38.0 36.0 40.0 31.0 40.0 25 36.40478380715247 38.0 36.0 40.0 31.0 40.0 26 35.923061545562696 38.0 35.0 40.0 30.0 40.0 27 35.81580700944605 38.0 35.0 40.0 30.0 40.0 28 35.4680797810442 38.0 35.0 40.0 29.0 40.0 29 35.41537403865532 38.0 35.0 40.0 29.0 40.0 30 35.04208138030568 38.0 34.0 39.0 28.0 40.0 31 34.58901417881824 38.0 34.0 39.0 27.0 40.0 32 34.419884151058696 38.0 33.0 39.0 26.0 40.0 33 34.15870232282978 38.0 33.0 39.0 26.0 40.0 34 34.344996781973855 38.0 33.0 39.0 26.0 40.0 35 34.1506523816644 38.0 33.0 39.0 26.0 40.0 36 34.21929222927949 38.0 33.0 39.0 26.0 40.0 37 34.00959231834405 38.0 33.0 39.0 25.0 40.0 38 33.75060622411765 37.0 33.0 39.0 24.0 40.0 39 33.66164730433426 38.0 33.0 39.0 23.0 40.0 40 33.41143276925777 37.0 33.0 39.0 23.0 40.0 41 32.83099511770175 37.0 32.0 39.0 18.0 40.0 42 32.72012924113093 37.0 32.0 39.0 17.0 40.0 43 32.535666456465634 36.0 32.0 39.0 17.0 40.0 44 32.362216472393236 36.0 32.0 39.0 15.0 40.0 45 31.888661171101475 36.0 31.0 39.0 8.0 40.0 46 31.072980932382446 36.0 30.0 38.0 2.0 40.0 47 30.89736609435643 35.0 30.0 38.0 2.0 40.0 48 30.607917644534883 35.0 30.0 38.0 2.0 40.0 49 30.34276529212526 35.0 30.0 38.0 2.0 40.0 50 29.94250459950202 35.0 29.0 38.0 2.0 40.0 51 29.15413045032863 35.0 28.0 38.0 2.0 40.0 52 29.017894175697727 35.0 28.0 38.0 2.0 40.0 53 28.67249595308833 35.0 28.0 38.0 2.0 40.0 54 28.34318948648104 34.0 27.0 38.0 2.0 40.0 55 27.89292749364521 34.0 26.0 38.0 2.0 40.0 56 16.065104894650275 20.0 2.0 23.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5840579288556853 1 2 -0.9280501563984203 1 3 -0.8367658702219032 1 4 -1.4661148526799934 1 5 -1.1751304823177406 1 6 -0.8963855818766362 1 7 -0.6480741281356401 1 8 -0.8478591852563326 1 9 -0.756328269397855 1 10 -0.600850060134384 1 11 -0.5680790303266647 1 12 -0.803426963825224 1 13 -0.7909495323080691 1 14 -0.6801208258257745 1 15 -0.9104660318845248 1 16 -0.40374586114393907 1 17 -0.5410111821882353 1 18 -0.7812271887748494 1 19 -0.536103561485902 1 20 -0.6974241889215236 1 21 -0.3582544452821139 1 22 -0.5466705916375858 1 23 -0.2904748896757994 1 24 -0.7807557020518487 1 25 -0.5571766294392191 1 26 -0.17679002988957393 1 27 -0.26436626548026254 1 28 -0.06349691287424974 1 29 -0.046841002751293104 1 30 -0.35870763244057713 1 31 0.030445145756765157 1 32 0.3486859853852451 1 33 -0.006471747218689927 1 34 -0.07403482360795266 1 35 -0.13676396281906733 1 36 0.3430192196395154 1 37 0.2851878141903228 1 38 0.16567213309504325 1 39 -0.2312962385394144 1 40 -0.00727217074280162 1 41 0.608514645167638 1 42 0.6398592807612644 1 43 0.4633020276829427 1 44 0.8117589476103433 1 45 0.4612587581014935 1 46 1.1690944875049034 1 47 0.9199280310885847 1 48 0.7534941012811167 1 49 1.096221964648901 1 50 1.1566673970022343 1 51 1.7747515011325348 1 52 2.1365957960933564 1 53 1.8971757651040022 1 54 1.7983465832971994 1 55 2.0935154937668585 1 56 1.5529508774737533 2 1 -0.3491770670794452 2 2 -0.355549196917778 2 3 -0.43838300166749633 2 4 -0.40336481852898487 2 5 -0.6805198363108076 2 6 -0.7214576242368906 2 7 -0.3472883297501639 2 8 -0.2253286282371647 2 9 -0.2526692330416722 2 10 -0.6170538993579555 2 11 -0.6692570774095188 2 12 -0.7576581108436784 2 13 -0.5143263837804639 2 14 -0.4055792624252419 2 15 -0.4827360655151338 2 16 -0.1791493308861476 2 17 -0.6111963782150056 2 18 -0.28686047852499286 2 19 -0.2741881128717836 2 20 -0.17969168562025573 2 21 -0.031152857422952707 2 22 0.2621130471033126 2 23 0.08636682529014195 2 24 0.2059969601003857 2 25 0.2172413398443922 2 26 0.15935344336531188 2 27 0.43969087403147 2 28 0.24649664722176112 2 29 0.01784750754930542 2 30 0.16522734404401973 2 31 -0.15251968195954646 2 32 0.19106770906782344 2 33 0.14933489954282209 2 34 -0.08714230495500175 2 35 0.11327344066368283 2 36 0.6581907227391284 2 37 0.8418849890890101 2 38 0.7573106658377213 2 39 0.8627491270064453 2 40 0.5743845108202095 2 41 0.8838205406070117 2 42 0.9690424537474911 2 43 0.3685346122961235 2 44 0.9893669461011925 2 45 0.34207499966944965 2 46 0.8286935221698037 2 47 1.2308680619220667 2 48 1.1698143791727311 2 49 0.8871137287272468 2 50 1.2008783136152346 2 51 0.9076346916821372 2 52 1.3614134947709005 2 53 1.2033183927149267 2 54 1.2390897091875672 2 55 1.3542828180843252 2 56 1.1583734067297815 3 1 -0.46796370745860827 3 2 -0.5128661745516396 3 3 -0.6790017589302906 3 4 -0.5703927207081492 3 5 -1.0016821442140298 3 6 -0.7474208826682585 3 7 -0.3052000403091242 3 8 -0.27924955683004526 3 9 -0.3259499913751114 3 10 -0.9754278172835029 3 11 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1.0268422248818645 3 51 1.3709584065759195 3 52 0.8883298926870502 3 53 1.0874042274953766 3 54 0.9169742617995595 3 55 1.0244932921732932 3 56 0.6950823335991725 4 1 -0.5023639552744186 4 2 -0.3711654753570457 4 3 -0.4869287038418477 4 4 -0.4566566445427185 4 5 -0.5150338530051641 4 6 -0.7540904152320138 4 7 -0.32647300436318716 4 8 -0.4266082954981627 4 9 -0.2749517741316865 4 10 -0.7652458749639024 4 11 -0.5248352082405034 4 12 -0.6543948317441703 4 13 -0.43157541812926326 4 14 -0.5937528659389102 4 15 -0.3557262192219284 4 16 -0.4163694036728032 4 17 -0.6144752863513574 4 18 -0.25884622739507535 4 19 -0.3564574490204677 4 20 -0.4822538907816707 4 21 -0.011170017467485138 4 22 0.033284810218148664 4 23 0.09264395681965709 4 24 -0.08798841239697452 4 25 0.17851322448296258 4 26 0.3177202409118678 4 27 0.2266479643513577 4 28 0.2636758293446988 4 29 0.30011429201850603 4 30 0.583654935354275 4 31 0.8230511765849116 4 32 0.5393273041800839 4 33 0.5809387257862042 4 34 1.1224111830313745 4 35 1.1812182861116511 4 36 1.1757446827862026 4 37 1.195270270363828 4 38 1.2151521363706124 4 39 1.1731705049771648 4 40 1.35064368490535 4 41 1.303310268502294 4 42 1.2142556625163365 4 43 1.5264165907408156 4 44 1.2655671125982835 4 45 1.582366656634413 4 46 2.0255268879307273 4 47 2.178625614095939 4 48 2.146836505423451 4 49 2.372497389685595 4 50 2.0986803607001008 4 51 1.9712353028093723 4 52 1.9242884720450029 4 53 2.1340498635348766 4 54 2.273279024667648 4 55 2.1393325279902626 4 56 1.37432523102113 5 1 -0.3174713353988068 5 2 -0.4733105849807444 5 3 -0.15440866744602744 5 4 -0.31906612241231613 5 5 -0.49305925529112216 5 6 -0.34757905400353195 5 7 -0.220616643197026 5 8 -0.10396000124047333 5 9 -0.2862413327700821 5 10 -0.049793856367131184 5 11 -0.7351269979923387 5 12 -0.5790885210585088 5 13 -0.558050882113605 5 14 -0.3651852656946062 5 15 -0.525733323884829 5 16 -0.27013799109132464 5 17 -0.3791489081090731 5 18 -0.19267003175509956 5 19 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1.8933374860403518 120 49 1.876161967765622 120 50 2.335021768203731 120 51 2.5926375849723335 120 52 2.4316908560902384 120 53 2.350619742040262 120 54 2.9412287712354903 120 55 2.9411078266326847 120 56 2.2867828862005712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 296.0 3 55.0 4 88.0 5 104.0 6 192.0 7 384.0 8 304.0 9 501.0 10 703.0 11 810.0 12 1121.0 13 2019.0 14 2701.0 15 2392.0 16 2520.0 17 3102.0 18 3447.0 19 3408.0 20 4294.0 21 4317.0 22 4600.0 23 5653.0 24 6799.0 25 7573.0 26 8378.0 27 9707.0 28 11229.0 29 12717.0 30 15403.0 31 18180.0 32 21921.0 33 27310.0 34 35979.0 35 49818.0 36 70301.0 37 97366.0 38 112809.0 39 66783.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 48.841835640127165 40.31650424844462 3.4645789586597404 7.377081152768477 11 33.53394530005656 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31.07035647127085 21.77996720197763 25.001259587082973 22.14841673966854 25 26.37941153995607 24.141104244605838 22.798640139302805 26.68084407613529 26 25.586673434724037 23.783864690914534 26.691688955701764 23.937772918659665 27 22.82235323024452 20.59405972815926 25.82373147224559 30.75985556935063 28 22.86599656267903 26.795479402114474 25.231596010624447 25.106928024582054 29 29.056906677347556 21.976746268608114 24.397856657890753 24.568490396153578 30 25.987923657704453 21.34019375588583 28.578015582587383 24.09386700382233 31 31.019118349280227 20.648827654543116 25.085927454249436 23.24612654192722 32 32.97566112038389 19.92179350093887 25.839440329647402 21.263105049029836 33 31.81741840067442 19.599981085034184 27.195644339941072 21.386956174350328 34 25.687605170816973 22.59623537391894 31.778707556645493 19.937451898618594 35 23.880528844193236 28.641920934367533 28.384839364799724 19.092710856639506 36 24.842236535831 30.856509518042003 25.96543404948533 18.33581989664167 37 28.911882096084117 26.317721812289708 24.871009956954364 19.899386134671808 38 25.05270377295329 28.945679596985556 24.311829976240436 21.68978665382072 39 28.961567425669305 24.294673150778472 24.96181572555022 21.781943698002003 40 25.361865172837106 24.185261891490303 24.44500384954255 26.007869086130043 41 22.603935725637154 27.378406482371638 26.263349617741504 23.754308174249708 42 22.24662866675935 24.042001619221303 25.875810632886548 27.8355590811328 43 23.15919307452217 20.990564494493775 27.583149574355314 28.26709285662874 44 24.343338513126685 21.0452277745972 25.82301463973174 28.788419072544368 45 26.568830216834982 23.32317771567325 25.810431379222326 24.297560688269442 46 23.187300673027675 26.62378358388465 26.3557506803094 23.83316506277828 47 23.283351398435375 22.16735379547248 27.384675335732556 27.164619470359586 48 23.965844277950133 21.643805548912525 28.969300472728822 25.42104970040852 49 26.321019587941098 23.007686200037654 27.444236168523418 23.227058043497838 50 22.073535313823296 23.097034179423545 28.368184387055557 26.461246119697602 51 21.418746766048585 23.23188823463914 30.743170222102872 24.606194777209403 52 21.256117802621294 24.295070202143503 28.006468854301676 26.442343140933527 53 21.367738467664886 23.662488385317403 31.5126208787337 23.45715226828401 54 21.708122068651324 23.03323096487462 30.37131235927068 24.887334607203375 55 20.95407212108393 23.322856051327204 27.019910115086137 28.703161712502727 56 21.722516118773186 24.93381180092557 24.98168041796516 28.361991662336088 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 239.5 9 479.0 10 1228.0 11 1977.0 12 2556.5 13 2654.0 14 2172.0 15 2042.5 16 1913.0 17 1956.5 18 2000.0 19 1989.5 20 1979.0 21 2321.5 22 3148.5 23 3633.0 24 4673.5 25 5714.0 26 6522.5 27 7331.0 28 8525.5 29 10306.5 30 10893.0 31 11429.5 32 11966.0 33 12647.0 34 13328.0 35 13508.5 36 13689.0 37 13858.5 38 14279.5 39 14531.0 40 16254.5 41 17978.0 42 18747.5 43 19517.0 44 20302.5 45 21088.0 46 22756.0 47 24173.5 48 23923.0 49 25261.5 50 26600.0 51 27688.0 52 28776.0 53 30384.5 54 34974.0 55 37955.0 56 45352.0 57 52749.0 58 53869.5 59 54990.0 60 53399.0 61 51808.0 62 42294.0 63 26671.5 64 20563.0 65 18936.0 66 17309.0 67 13868.5 68 10428.0 69 8868.5 70 7309.0 71 6515.5 72 4387.0 73 3052.0 74 2543.0 75 2034.0 76 1718.0 77 1402.0 78 1100.5 79 629.5 80 460.0 81 363.5 82 267.0 83 190.0 84 113.0 85 79.0 86 45.0 87 33.0 88 15.5 89 10.0 90 11.0 91 12.0 92 8.0 93 4.0 94 2.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0016252657309470098 23 0.0030880048887993185 24 4.875797192841029E-4 25 0.03591837265392892 26 0.10271679419585102 27 0.11913197807841581 28 0.13847264027668524 29 0.1792668101234552 30 0.2473654442501349 31 0.2738572756645712 32 0.28929730010856775 33 0.3271659916396331 34 0.3244030398970232 35 0.3655222628899825 36 0.4319956312857152 37 0.39753999778963867 38 0.4711645354015382 39 0.5072454346285618 40 0.5725811170126316 41 0.5941971512342268 42 0.6304405770343451 43 0.6462056546245311 44 0.6393795385545537 45 0.6709096937349257 46 0.6769231769394296 47 0.7362453761189954 48 0.7778521788312389 49 0.7230807236983247 50 0.784840821474311 51 0.7437215984813517 52 0.8085697011461375 53 0.8231970927246604 54 0.824009725590134 55 0.8765058086997225 56 0.8583028325131159 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 615284.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.244557697603263 #Duplication Level Percentage of deduplicated Percentage of total 1 62.33680543528851 15.11328276060121 2 10.963788413451082 5.316244015484578 3 5.734832874698345 4.17115459550208 4 4.012307528078141 3.8910648546007365 5 2.8678277254676336 3.476460737844319 6 2.282572406906332 3.320397505091863 7 1.7803785080192491 3.0215142622871847 8 1.5158803091734547 2.9401478094733196 9 1.2135624853268348 2.6480057125637937 >10 6.58035077702712 27.59315533387306 >50 0.3935167475326069 6.553196171083327 >100 0.29126966099422014 13.159558982746344 >500 0.015471598620940103 2.505844884990675 >1k 0.010762851214567026 4.694973156466746 >5k 6.726782009104391E-4 1.5949992173907455 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 9780 1.5895098848661755 No Hit CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 3366 0.5470644450367634 No Hit CATGATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 2399 0.38990124885418764 No Hit CATGATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 2338 0.3799871278954109 No Hit CATGATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 2110 0.3429310692298191 No Hit CATGATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 2063 0.3352923202943681 No Hit CATGATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 2050 0.333179474844137 No Hit CATGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 1868 0.30359963854090144 No Hit CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 1831 0.2975861553363975 No Hit CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1826 0.296773522470924 No Hit CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 1673 0.27190695678743476 No Hit CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 1437 0.2335506855370853 No Hit CATGATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 1419 0.2306252072213807 No Hit CATGATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 1189 0.19324409540959947 No Hit CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1151 0.18706808563200084 No Hit CATGATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 1041 0.1691901625915837 No Hit CATGATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCA 1027 0.1669147905682579 No Hit CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 909 0.1477366549430832 No Hit CATGATGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGC 898 0.1459488626390415 No Hit CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 870 0.14139811859238985 No Hit CATGATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 793 0.12888357246409787 No Hit CATGATGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC 781 0.12693325358696148 No Hit CATGATGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCA 746 0.12124482352864695 No Hit CATGATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 724 0.11766923892056352 No Hit CATGATGGGGTTTTGAGACAAGGTCTCTCAGTGTAGCTGCAGCTCTCCTGAAATTA 715 0.11620649976271119 No Hit CATGATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCT 696 0.11311849487391189 No Hit CATGATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 680 0.11051806970439668 No Hit CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTC 658 0.10694248509631324 No Hit CATGATGGGAGAGTCCTGTGCTGTAAGACAGCTTGACACAACTCGATGTCATTCTT 629 0.10222921447656692 No Hit CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC 617 0.10027889559943051 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.001462739157852309 0.0 0.0 0.0 0.0 11 0.0026004251695152156 0.0 0.0 0.0 0.0 12 0.0026004251695152156 0.0 0.0 0.0 0.0 13 0.0026004251695152156 0.0 0.0 0.0 0.0 14 0.0039006377542728237 0.0 0.0 0.0 0.0 15 0.0039006377542728237 0.0 0.0 0.0 0.0 16 0.0042256909004622254 0.0 0.0 0.0 0.0 17 0.004713270619746328 0.0 0.0 0.0 0.0 18 0.005038323765935731 0.0 0.0 0.0 0.0 19 0.005200850339030431 0.0 0.0 0.0 0.0 20 0.00666358949688274 0.0 0.0 0.0 0.0 21 0.007313695789261544 0.0 0.0 0.0 0.0 22 0.00828885522782975 0.0 0.0 0.0 0.0 23 0.009589067812587358 0.0 0.0 0.0 0.0 24 0.010726753824250266 0.0 0.0 0.0 0.0 25 0.011051806970439667 0.0 0.0 0.0 0.0 26 0.012514546128291976 0.0 0.0 0.0 0.0 27 0.014302338432333686 0.0 0.0 0.0 0.0 28 0.01836550275970121 0.0 0.0 0.0 0.0 29 0.01966571534445882 0.0 0.0 0.0 0.0 30 0.022591193660163437 0.0 0.0 0.0 0.0 31 0.02307877337944754 0.0 0.0 0.0 0.0 32 0.02307877337944754 0.0 0.0 0.0 0.0 33 0.023403826525636943 0.0 0.0 0.0 0.0 34 0.024053932818015746 0.0 0.0 0.0 0.0 35 0.03201773489965609 0.0 0.0 0.0 0.0 36 0.033805527203697804 0.0 0.0 0.0 0.0 37 0.03461816006917131 0.0 0.0 0.0 0.0 38 0.035430792934644816 0.0 0.0 0.0 0.0 39 0.03689353209249712 0.0 0.0 0.0 0.0 40 0.03835627125034943 0.0 0.0 0.0 0.0 41 0.03949395726201234 0.0 0.0 0.0 0.0 42 0.04111922299295935 0.0 0.0 0.0 0.0 43 0.04176932928533815 0.0 0.0 0.0 0.0 44 0.042581962150811656 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCATT 60 0.0 50.171753 41 AACGACC 15 0.0083317915 50.147144 40 GTACGAT 20 4.122151E-4 50.14714 40 CGGCAAT 15 0.00834526 50.126656 36 ATTCGTT 35 5.1797542E-8 50.126656 36 TATCCGT 45 1.364242E-10 50.004074 27 AGCGTAC 15 0.00843728 49.987778 26 ACTCATC 15 0.00843728 49.987778 26 CGTATCC 45 1.364242E-10 49.97556 25 ACGTATC 45 1.364242E-10 49.96742 24 ATACGCC 15 0.008450875 49.96742 24 ATATGCG 15 0.008483571 49.91863 22 CGATTCA 15 0.0084890295 49.910507 21 TCGCAAC 15 0.0085273115 49.853718 16 GTCGATA 15 0.0085273115 49.853718 10 GGTCGAT 15 0.0085273115 49.853718 9 GTTACGG 85 0.0 49.853718 12 CTAACGG 25 2.1228032E-5 49.853718 18 ACGTGTA 15 0.0085273115 49.853718 14 CGTCACT 15 0.0085273115 49.853718 14 >>END_MODULE