FastQCFastQC Report
Tue 31 May 2016
SRR212776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257003
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19400.7548550016925872No Hit
TCAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15720.6116660116807975No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA14870.5785924677922047No Hit
TCAAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT13450.5233401944724381No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA12640.4918230526491908No Hit
TCAAAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC6970.2712030598864605No Hit
TCAAAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA6790.26419925059240557No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6140.2389077170305405No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA5940.23112570670381277No Hit
TCAAAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5110.19883036384789285No Hit
TCAAAGGGGAGCATTAGAACATCTATGCATATGGTGATCTAAATGCACAAAATGTAAA4930.1918265545538379No Hit
TCAAAGGGGATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTG4640.1805426395800827No Hit
TCAAAGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT4350.16925872460632757No Hit
TCAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4340.16886962408999118No Hit
TCAAAGGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAGA4100.15953121169791792No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4040.15719660859989962No Hit
TCAAAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG3900.15174920137119022No Hit
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3690.14357809052812615No Hit
TCAAAGGGGCAAATCCTGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCT3580.1392979848484259No Hit
TCAAAGGGGAACATAGATGTCCAGATTGTGATTTTTGTCTTGAATGAGACCGTTAAAC3460.13462877865238926No Hit
TCAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3420.13307237658704374No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA3390.13190507503803459No Hit
TCAAAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG3370.1311268740053618No Hit
TCAAAGGGGACAGTAATATACCAATGTCTCTTGGACCTTATTTCCCTTGTCGAGAAAA3330.12957047194001625No Hit
TCAAAGGGGAGCTTGCACAGCTGGCAGGGGCTGCCTAGTACGGACTAAAGCTAGAAAG3300.1284031703910071No Hit
TCAAAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA3260.12684676832566158No Hit
TCAAAGGGGATCACCTACCACTGCAAGAACAGCATTGCGTACCTGGATGAGGAGACGG3150.12256666264596133No Hit
TCAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG3050.11867565748259748No Hit
TCAAAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.11750835593358833No Hit
TCAAAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC3010.11711925541725195No Hit
TCAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2980.11595195386824278No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2950.11478465231923364No Hit
TCAAAGGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG2840.11050454663953338No Hit
TCAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2730.10622444095983316No Hit
TCAAAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2580.10038793321478737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAAC251.6383652E-552.07056452
CGCATTC150.00719181152.0401343
AACCGAA150.00721400351.9996135
CGCATTG203.4427491E-451.9996125
CAACCGA150.00721400351.9996134
TTATAGG150.00721400351.9996124
CCGTAAT150.00721400351.9996128
ACGGCCA150.00721400351.9996126
ATCCCGT150.00721400351.9996124
CGTAATG150.00721400351.9996129
TATAGGT150.00721400351.9996125
CGTAACG150.00721400351.9996129
TAGTACG203.4427491E-451.9996136
CTTACGC150.00721400351.9996133
TACGCAT203.4460597E-451.9894923
CGCGTTG203.449373E-451.97937822
ACGCGTT203.449373E-451.97937821
CAACGCG150.007225117651.9793722
GTCATAG150.0072306851.9692611
AGGTATA203.4526884E-451.9692619
Produced by FastQC (version 0.11.5)