FastQCFastQC Report
Tue 31 May 2016
SRR212775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275938
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20740.7516181171132645No Hit
TCAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17210.6236908290992904No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15730.5700555921982474No Hit
TCAAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT13560.49141473809333985No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA13550.4910523378440085No Hit
TCAAAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA7800.2826721944784698No Hit
TCAAAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC7760.2812225934811443No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6940.2515057730359719No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA6460.23411056106806602No Hit
TCAAAGGGGAGCATTAGAACATCTATGCATATGGTGATCTAAATGCACAAAATGTAAA5780.20946734411353274No Hit
TCAAAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5190.18808572940298185No Hit
TCAAAGGGGATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTG4850.1757641209257152No Hit
TCAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4670.1692409164377505No Hit
TCAAAGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT4500.1630801121991172No Hit
TCAAAGGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAGA4240.15365770571650153No Hit
TCAAAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG4200.15220810471917604No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4100.14858410222586232No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA4080.1478593017271996No Hit
TCAAAGGGGAACATAGATGTCCAGATTGTGATTTTTGTCTTGAATGAGACCGTTAAAC3930.14242329798722903No Hit
TCAAAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA3880.14061129674057216No Hit
TCAAAGGGGACAGTAATATACCAATGTCTCTTGGACCTTATTTCCCTTGTCGAGAAAA3850.13952409599257803No Hit
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3690.13372569200327608No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA3610.13082649000862515No Hit
TCAAAGGGGCAAATCCTGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCT3600.13046408975929377No Hit
TCAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3420.12394088527132906No Hit
TCAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3320.12031688277801536No Hit
TCAAAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG3160.11451847878871341No Hit
TCAAAGGGGAGCTTGCACAGCTGGCAGGGGCTGCCTAGTACGGACTAAAGCTAGAAAG3130.11343127804071929No Hit
TCAAAGGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG3030.10980727554740558No Hit
TCAAAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC2970.10763287405141735No Hit
TCAAAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2920.1058208728047605No Hit
TCAAAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2870.10400887155810362No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACTGCTCTTCCAA2800.10147206981278402No Hit
TCAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG2800.10147206981278402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACA203.4075728E-452.10923452
GACCGGT150.007185629652.05246727
ACGTAAA150.007185629652.05246724
CGTTCGC203.4351854E-452.02413642
CATTAGG150.00720112152.0241342
CGTAATG150.00720112152.0241342
CTGATCG150.00720112152.0241343
ATTAGGC203.4382645E-452.01469836
GCGTTCG203.4382645E-452.01469841
CGCGTTC203.4382645E-452.01469840
ATACGTA150.007206290552.01469422
AATACTC150.007206290552.01469422
TACGTAA150.007206290552.01469423
GTACGGA550.052.01469438
TAGTACG550.052.01469436
ACGGCCC150.007216637551.99582730
CGATTAG203.4475146E-451.98640432
ATCTACG150.00722181551.98639731
GTCTCGT150.00722181551.98639734
ATTGGGT150.00722181551.98639731
Produced by FastQC (version 0.11.5)