FastQCFastQC Report
Tue 31 May 2016
SRR212774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290211
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19130.6591755653645106No Hit
TCAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16450.5668289623756508No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA14440.4975690101340059No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA13760.4741377825099669No Hit
TCAAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT13610.4689691293576053No Hit
TCAAAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC7280.2508519663279476No Hit
TCAAAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA7010.2415483906536968No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6090.20984731798587924No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA6080.20950274110905515No Hit
TCAAAGGGGAGCATTAGAACATCTATGCATATGGTGATCTAAATGCACAAAATGTAAA5070.17470047654982063No Hit
TCAAAGGGGATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTG5040.17366674591934833No Hit
TCAAAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA4880.16815351589016267No Hit
TCAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.15574874832449495No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4310.14851263391118874No Hit
TCAAAGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT4270.14713432640389235No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA3700.12749344442491842No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCAAA3570.12301394502620507No Hit
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3550.12232479127255687No Hit
TCAAAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG3510.12094648376526046No Hit
TCAAAGGGGAACATAGATGTCCAGATTGTGATTTTTGTCTTGAATGAGACCGTTAAAC3500.12060190688843635No Hit
TCAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3430.11818986875066761No Hit
TCAAAGGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCAGA3370.116122407489723No Hit
TCAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3360.11577783061289888No Hit
TCAAAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA3300.11371036935195428No Hit
TCAAAGGGGACAGTAATATACCAATGTCTCTTGGACCTTATTTCCCTTGTCGAGAAAA3180.10957544683006502No Hit
TCAAAGGGGAGCTTGCACAGCTGGCAGGGGCTGCCTAGTACGGACTAAAGCTAGAAAG3080.10612967806182397No Hit
TCAAAGGGGCAAATCCTGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCT2960.10199475553993474No Hit
TCAAAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2940.10130560178628653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAC307.6374454E-752.3260889
ACGGGTT150.00719955652.02765343
GAGCGTA150.00719955652.02765342
TAATCCG150.00719955652.02765342
GCAACGA203.4460652E-451.9917124
CTCGGTG150.00721926151.99170726
ACACGAT203.4548907E-451.96478729
CACACGA203.4548907E-451.96478728
CTTACGC203.4548907E-451.96478733
AGTACGG505.456968E-1251.96478337
TAGTACG505.456968E-1251.96478336
CTTGTCG401.900844E-951.95581446
TCGACGT203.4607842E-451.9468545
CGATTAG203.4725954E-451.9110232
TATTCGG203.4725954E-451.9110232
GCTTACG203.4725954E-451.9110232
CCGAACC308.0786776E-751.9020722
CGCGTTG150.00726869951.9020722
GCGAAAG150.00727365751.89312415
TCCGAAC308.0883E-751.89312421
Produced by FastQC (version 0.11.5)