FastQCFastQC Report
Tue 31 May 2016
SRR212773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444229
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC48801.0985325136359851No Hit
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32500.7316046453518343No Hit
TCAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30220.6802797656163826No Hit
TCAAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC24230.5454394017499983No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC14930.33608791861855036No Hit
TCAAAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC11970.26945561861112177No Hit
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11090.24964601590621052No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10540.23726501421564103No Hit
TCAAAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC9590.2158796476592028No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA9330.2100268104963881No Hit
TCAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8320.18729078921006959No Hit
TCAAAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA8170.18391415238536882No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC7130.1605028037341101No Hit
TCAAAGGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCA6800.1530742027197684No Hit
TCAAAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC6740.1517235479898881No Hit
TCAAAGGGGAGCATTAGAACATCTATGCATATGGTGATCTAAATGCACAAAATGTA6570.1478966929218939No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCA6180.1391174371776719No Hit
TCAAAGGGGAACATAGATGTCCAGATTGTGATTTTTGTCTTGAATGAGACCGTTAA6100.13731656420449814No Hit
TCAAAGGGGCAAATCCTGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTT5960.13416503650144407No Hit
TCAAAGGGGATTGTCCTCTCTGGTGGTTCAACCATGTTCAGGGACTTTGGACGTTT5870.1321390544066236No Hit
TCAAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT5670.12763687197368925No Hit
TCAAAGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG5480.12335979866240158No Hit
TCAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5340.12020827095934755No Hit
TCAAAGGGGGATAGAGTCCTGTGCTGTAAGACAGCTTGACACAACTCGATGTCATT5270.11863250710782051No Hit
TCAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5250.11818228886452707No Hit
TCAAAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.11007836048524522No Hit
TCAAAGGGGATTCCTGTTGATATGTGTGACTTTCCAGGAGAGGATTAATCAGTGTG4570.10287486859255023No Hit
TCAAAGGGGACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACAAATGCT4550.10242465034925681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGT204.081506E-450.2448449
GTATCCG252.0273339E-550.2391448
ACGGTAA150.00829984250.1935946
CGTAGTG150.0083184550.16515743
ACGTAGT150.0083184550.16515742
AACTCGA550.050.16515443
TAGTACG600.050.10839536
TAGCGGC150.00836697750.09139334
CTTACGC204.1435784E-450.09139333
TACTAGC150.00839319450.05176530
CGCTATA150.00846466249.94451521
ACGCGTT204.2040588E-449.94450821
GCTACGT204.2157696E-449.91636320
CTCGCTA150.00851003349.87701419
CTATACG150.00852899149.8489418
GCTATAC150.00852899149.8489417
TCGATAT301.0684762E-649.8489414
TTGCGTT150.00852899149.8489412
CTCGATA301.0684762E-649.8489413
CGCCCAT150.00852899149.8489415
Produced by FastQC (version 0.11.5)