FastQCFastQC Report
Tue 31 May 2016
SRR212772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406845
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC46001.1306517224004227No Hit
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30270.7440179921100174No Hit
TCAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26690.656023792844941No Hit
TCAAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC23690.5822856370362177No Hit
TCAAAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC19250.4731531664393073No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC13480.33133011343386304No Hit
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10570.2598041022994015No Hit
TCAAAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC9870.2425985326106994No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9140.2246555813639101No Hit
TCAAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT8820.21679017807764628No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA8420.2069584239698165No Hit
TCAAAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA7840.19270238051346336No Hit
TCAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7560.18582015263798252No Hit
TCAAAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC6650.16345291204266982No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC6450.15853703498875493No Hit
TCAAAGGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAGCA6100.1499342501444039No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCA5920.1455099607958805No Hit
TCAAAGGGGCAAATCCTGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTT5760.1415772591527486No Hit
TCAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5480.13469503127726776No Hit
TCAAAGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG5480.13469503127726776No Hit
TCAAAGGGGAGCATTAGAACATCTATGCATATGGTGATCTAAATGCACAAAATGTA5470.13444923742457202No Hit
TCAAAGGGGATTGTCCTCTCTGGTGGTTCAACCATGTTCAGGGACTTTGGACGTTT5350.1314997111922231No Hit
TCAAAGGGGGATAGAGTCCTGTGCTGTAAGACAGCTTGACACAACTCGATGTCATT4980.12240533864248054No Hit
TCAAAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA4760.11699787388317417No Hit
TCAAAGGGGAACATAGATGTCCAGATTGTGATTTTTGTCTTGAATGAGACCGTTAA4610.11331096609273802No Hit
TCAAAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4490.1103614398603891No Hit
TCAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4480.11011564600769334No Hit
TCAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4450.1093782644496061No Hit
TCAAAGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCT4340.10667453206995292No Hit
TCAAAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4280.10519976895377846No Hit
TCAAAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4150.10200444886873379No Hit
TCAAAGGGGATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTT4090.10052968575255933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTT150.00821748550.31958849
TATCCGT204.0512564E-450.31958849
CGATATC150.00825385850.263446
CCGATTA150.00826195750.2509344
AGAACGG150.00826195750.2509344
CGTTAGG150.00826195750.2509345
ATCGATA150.00826195750.2509344
CGTACTC150.00827817350.2260141
CCGTTAT150.008339195550.13278233
CGATTAG301.0316126E-650.1017832
CTATTAA150.00838006350.0708230
CGAACGT150.00839645150.04607429
CGAACCG150.00839645150.04607429
ACGCTCT150.00840875850.0275427
ACTCGAA150.0084169750.01518226
ATTCGAC204.1998943E-449.95351423
CGATGCG150.00849528349.8981422
ACGCGTT150.00850769849.87970721
CGTTGTC150.008565810549.79387713
GGGCGAT204.2667554E-449.7938777
Produced by FastQC (version 0.11.5)