FastQCFastQC Report
Tue 31 May 2016
SRR212771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298752
Sequences flagged as poor quality0
Sequence length58
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61942.073291559554413No Hit
GCCTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48831.6344660454155957No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22620.7571497429305912No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT15700.5255194944301628No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15580.5215027849185947No Hit
GCCTAGGGGATGTGGAGAGAGAGTGTTATGTGCACGTATGTGGAGAGAGAGTATTGTG11630.3892860968294773No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA8280.2771529562982005No Hit
GCCTAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7880.26376392459297343No Hit
GCCTAGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7210.2413372964867181No Hit
GCCTAGGGGATGCCAAGCCCCTCACACTGAAGGAGGTTGAGGCATTTAAAAGCTCCAA6160.206191088260497No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5390.18041720222793486No Hit
GCCTAGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5360.17941302485004285No Hit
GCCTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4910.16435036418166238No Hit
GCCTAGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4470.1496224293059126No Hit
GCCTAGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4110.13757230077120822No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA4060.13589867180805484No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA3970.13288613967437876No Hit
GCCTAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3960.13255141388174807No Hit
GCCTAGGGGTTTTGGATTCTTTTGAACTTGTAATACTATATATAATTATTTCTAAAGC3480.11648457583547558No Hit
GCCTAGGGGAAGGCGCTTTTCAAAGGCTCTGCTTCACTCAGTCTTAAACTGCAATTGT3430.1148109468723222No Hit
GCCTAGGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAG3320.11112896315338475No Hit
GCCTAGGGGGGGGTTTAATGAGGTGACCTGTAGCTTTGGCACGTCCAGAGTTCTTTGT3230.10811643101970865No Hit
GCCTAGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.10108718937446445No Hit
GCCTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.10008301199657241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAT150.00720272452.02227448
GCGCAAC150.00720749652.0135642
CGTATGT1350.052.0048635
CCTTCGG150.007212270552.00485629
CACGCTA251.6513131E-552.00485635
TGACGCG150.007212270552.00485639
CGCTAAC251.6513131E-552.00485637
ACGCTAA251.6513131E-552.00485636
CACGTCC401.886292E-952.00485240
GGCTTGA203.4447905E-451.9961524
TCGCGCT203.4447905E-451.9961527
TTACTTG150.007217047351.99614731
TGACGTA150.007217047351.99614725
CGCTATA150.007217047351.99614732
ACGCTCT150.007217047351.99614723
ACGCTAT150.007217047351.99614731
TTCGGTA150.007217047351.99614725
ACGTAAC150.007217047351.99614726
GAACCAC150.007217047351.99614727
GCGTACC150.007217047351.99614727
Produced by FastQC (version 0.11.5)