FastQCFastQC Report
Tue 31 May 2016
SRR212770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212770_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301010
Sequences flagged as poor quality0
Sequence length58
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58801.9534234743031793No Hit
GCCTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46771.553768977774825No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22300.7408391747782466No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT16930.5624397860536196No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15660.5202484967276835No Hit
GCCTAGGGGATGTGGAGAGAGAGTGTTATGTGCACGTATGTGGAGAGAGAGTATTGTG11730.3896880502308893No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA7890.2621175376233348No Hit
GCCTAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7300.24251685990498653No Hit
GCCTAGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7110.23620477724992525No Hit
GCCTAGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6000.199328925949304No Hit
GCCTAGGGGATGCCAAGCCCCTCACACTGAAGGAGGTTGAGGCATTTAAAAGCTCCAA5920.19667120693664664No Hit
GCCTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5260.1747450250822232No Hit
GCCTAGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4980.16544300853792235No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4940.16411414903159363No Hit
GCCTAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.14683897544932062No Hit
GCCTAGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4100.13620809939869108No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA4040.13421481013919803No Hit
GCCTAGGGGTTTTGGATTCTTTTGAACTTGTAATACTATATATAATTATTTCTAAAGC3700.12291950433540415No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA3650.12125842995249327No Hit
GCCTAGGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAG3400.11295305803793894No Hit
GCCTAGGGGAAGGCGCTTTTCAAAGGCTCTGCTTCACTCAGTCTTAAACTGCAATTGT3280.10896647951895286No Hit
GCCTAGGGGGGGGTTTAATGAGGTGACCTGTAGCTTTGGCACGTCCAGAGTTCTTTGT3230.107305405136042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAT203.4128557E-452.09443325
CAACGGG150.007168111452.08576221
ACGCTCT203.4156637E-452.08576223
TGACGGA150.007196479552.03377551
CCGATGA150.007201215352.02511650
CGACGCG150.007201215352.02511650
CCACCCG150.00720595452.01646449
CAGCGTA251.6507844E-552.00781636
GGCACGT251.6507844E-552.00781638
GCGTAGA251.6507844E-552.00781638
CGTATTG150.007210694752.00781237
TAAGTAG150.007210694752.00781248
CTATTAG150.007210694752.00781239
CGTGGTT150.007215437551.99917233
GTAAGGA150.007215437551.99917232
CCACGGC150.007215437551.99917233
TGACATA203.4438417E-451.9991745
TCCACGG251.65241E-551.9991732
ACGTACT251.65241E-551.9991741
CGTCGTC150.00722018351.99052429
Produced by FastQC (version 0.11.5)