FastQCFastQC Report
Tue 31 May 2016
SRR212769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306047
Sequences flagged as poor quality0
Sequence length58
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54311.774564037549788No Hit
GCCTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42541.3899825843742954No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19330.6316023355889782No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT16270.5316176927073293No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15400.5031906863978409No Hit
GCCTAGGGGATGTGGAGAGAGAGTGTTATGTGCACGTATGTGGAGAGAGAGTATTGTG11120.36334288524311625No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA7040.2300300280675844No Hit
GCCTAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6620.21630664571127964No Hit
GCCTAGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5870.19180060578930685No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5440.17775047623404247No Hit
GCCTAGGGGATGCCAAGCCCCTCACACTGAAGGAGGTTGAGGCATTTAAAAGCTCCAA5280.1725225210506883No Hit
GCCTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5240.17121553225484973No Hit
GCCTAGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5170.16892830186213229No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA4320.14115478995056313No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA4220.13788731796096676No Hit
GCCTAGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4030.13167912118073366No Hit
GCCTAGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4030.13167912118073366No Hit
GCCTAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3870.1264511659973795No Hit
GCCTAGGGGTTTTGGATTCTTTTGAACTTGTAATACTATATATAATTATTTCTAAAGC3700.12089646361506566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC203.3547872E-452.276048
GATCGAA203.3547872E-452.276049
GGATTCG458.731149E-1152.276048
GGTCGTG150.007181403752.0615524
CGAACGG203.4235924E-452.06154627
TCGGTAG401.8681021E-952.06154626
CGCGCGA150.00718606652.05300529
AGTAGAT150.00719539852.0359336
TAGTACA203.431924E-452.03592337
TTAGCCG150.007209412852.0103330
GCGCGAC150.007209412852.0103330
GCAATTG459.276846E-1152.0103351
TACGTTC150.007209412852.0103351
ATACGTT150.00721408952.0018150
CGTCCAG307.9737583E-752.0018142
CGTCATA150.00721408952.0018143
AATACGT150.00723281651.96772849
CTATACG150.00724219351.95070623
CCACTAG150.00724219351.95070623
AACGCGA251.661613E-551.95070623
Produced by FastQC (version 0.11.5)