FastQCFastQC Report
Tue 31 May 2016
SRR212768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346194
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58941.7025136195312454No Hit
GCCTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45831.3238242141689343No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT41761.2062600738314355No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC26150.7553568230529704No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21310.6155508183272962No Hit
GCCTAGGGGATGTGGAGAGAGAGTGTTATGTGCACGTATGTGGAGAGAGAGTATTG14480.41826259265036364No Hit
GCCTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13640.39399874059053597No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8920.2576590004448373No Hit
GCCTAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7720.22299635464508338No Hit
GCCTAGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7510.21693039163012645No Hit
GCCTAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7140.20624274250853566No Hit
GCCTAGGGGATTAACATTTCATTTGAAATGCATACTCTTCTTGAAATATTTTGTTT5770.16666955522048332No Hit
GCCTAGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5360.15482648457223405No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA4670.13489546323737556No Hit
GCCTAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG4650.13431775247404634No Hit
GCCTAGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4590.13258462018405864No Hit
GCCTAGGGGATGCCAAGCCCCTCACACTGAAGGAGGTTGAGGCATTTAAAAGCTCC4570.13200690942072943No Hit
GCCTAGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4150.11987498339081555No Hit
GCCTAGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT4040.11669757419250479No Hit
GCCTAGGGGTTTTGGATTCTTTTGAACTTGTAATACTATATATAATTATTTCTAAA3980.11496444190251709No Hit
GCCTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.10803191274256631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCCG150.00826782250.2403150
CGCATGA150.00827258350.23299849
AGCTCGA150.00827258350.23299849
ACTCGTT402.564775E-950.23299448
TGCGCAT204.087919E-450.2256847
ACGGGCA150.00829164950.20375445
CGGATAT150.00829642150.19644544
GACGGGC150.00829642150.19644544
GTCAATT301.0188432E-650.1891442
CGTACTG301.0188432E-650.1891442
TTCGGAT150.00830119450.1891443
GCGGATA150.00830119450.1891443
ACGTACT301.0198655E-650.18184341
CTACGTA355.1379175E-850.16724839
TCTACGT301.0239673E-650.1526638
TAAGCGT301.0239673E-650.1526637
CGCGTCC150.00832987950.14536736
AGTCCGG150.00834424850.1235134
ACGATTC150.00834904250.11622633
AACGATT150.00834904250.11622632
Produced by FastQC (version 0.11.5)