FastQCFastQC Report
Tue 31 May 2016
SRR212767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319089
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54011.6926312094744724No Hit
GCCTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41471.299637405238037No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT38151.1955911986937815No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC23040.7220556020420635No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18200.5703737828630884No Hit
GCCTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12070.3782643713822789No Hit
GCCTAGGGGATGTGGAGAGAGAGTGTTATGTGCACGTATGTGGAGAGAGAGTATTG11640.3647885072816675No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8400.2632494382445023No Hit
GCCTAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7020.22000131624719121No Hit
GCCTAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6800.21310668810269234No Hit
GCCTAGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6170.19336298023435466No Hit
GCCTAGGGGATTAACATTTCATTTGAAATGCATACTCTTCTTGAAATATTTTGTTT5530.1733058801776307No Hit
GCCTAGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT5160.16171036920733714No Hit
GCCTAGGGGATGCCAAGCCCCTCACACTGAAGGAGGTTGAGGCATTTAAAAGCTCC4620.14478719103447626No Hit
GCCTAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG4610.14447379884608996No Hit
GCCTAGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.14133987696222683No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA4430.13883273945513633No Hit
GCCTAGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4030.12629705191968385No Hit
GCCTAGGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3870.12128277690550285No Hit
GCCTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.11250779563068611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGT150.00822874750.29963749
TAGCGGA150.00825450950.2598842
CCGTGAG150.00825450950.2598842
CGTACTG204.0737228E-450.2598842
GTAGCGG150.00825966950.25193441
ACGTACT204.0769018E-450.25193441
AACTAGC150.00829585550.196440
CACGTCC252.0362526E-550.19639640
GCGCACT204.1088025E-450.17263839
TCGAGAC150.00831139850.17263839
GGCACGT252.0457823E-550.15680738
TAAGCGT204.1152065E-450.15680337
TAAGCGC204.1184112E-450.14889536
AATGCCG150.00835295450.1093835
CGTTGCA150.00839466550.04627633
CGCTATA204.16996E-450.02265532
CCGCGGT150.00841557850.0147931
CGGTAGG150.00841557850.0147931
ACGCGCC150.0084260549.99905829
CCCGATG204.179682E-449.99905429
Produced by FastQC (version 0.11.5)