FastQCFastQC Report
Tue 31 May 2016
SRR212766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569778
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA58801.0319808767625287No Hit
ATCTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37840.6641183057260899No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA34700.6090091228513561No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG28050.4922969998841654No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA26210.4600037207473788No Hit
ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24270.42595537209228856No Hit
ATCTCTGGGGGTGTTCGTCAAGGTCTGAGATTGACAAGGGGCTCCCTTCCCCATCTCT14690.2578197122388018No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA14680.2576442052869714No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC10570.18551084808469265No Hit
ATCTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10550.1851598341810319No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA10530.18480882027737117No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA10140.17796404915598707No Hit
ATCTCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC9220.16181740958759377No Hit
ATCTCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8840.15514814541804No Hit
ATCTCTGGGACCAAGGGATCTACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGA8140.14286265878991466No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT7680.13478933900571802No Hit
ATCTCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG7050.12373240104040521No Hit
ATCTCTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT6650.11671212296719073No Hit
ATCTCTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA6430.11285097002692275No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG6360.11162242136411023No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCTGA6040.10600619890553865No Hit
ATCTCTGGGGAACTTGTAATACTATATATAATTATTTCTAAAGCACAGATTAAGTAAG5880.10319808767625285No Hit
ATCTCTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5820.10214504596527069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC150.007190014252.0516148
CGTTATA203.4293506E-452.0516146
ACGCTAA251.6444523E-552.0516146
CGCTACA251.6495937E-552.02416634
CGGATTA150.007212538752.01045629
GCGTGTA150.007212538752.01045626
TCGACGC150.007212538752.01045627
TTCGACG150.007212538752.01045626
TACGGTA251.6521692E-552.01045230
CGCCACA150.0072401451.96024319
GAATCCG150.00724265351.9556812
CCGCTAG150.00724265351.9556810
GGTATCG251.6625052E-551.955688
ACGAACG150.00724265351.9556810
ATCTCTG590200.050.1554531
CTCTGGG591550.050.0409933
TCTCTGG592200.049.986072
TCTGGGG286800.049.827094
TCTGGGA223150.049.732174
CTGGGGG121750.049.459255
Produced by FastQC (version 0.11.5)