FastQCFastQC Report
Tue 31 May 2016
SRR212765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575615
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA59571.0348931143212043No Hit
ATCTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37510.6516508430113879No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA33800.5871980403568358No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG27730.48174561121583004No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA26400.4586398895094812No Hit
ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25270.4390087124206284No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA14870.2583323923108327No Hit
ATCTCTGGGGGTGTTCGTCAAGGTCTGAGATTGACAAGGGGCTCCCTTCCCCATCTCT13990.24304439599385005No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11110.19301095350190667No Hit
ATCTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10910.1895364088844106No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA10590.18397713749641686No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA10560.18345595580379245No Hit
ATCTCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC9380.16295614256056565No Hit
ATCTCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9220.16017650686656879No Hit
ATCTCTGGGACCAAGGGATCTACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGA7720.13411742223534828No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT7390.12838442361647978No Hit
ATCTCTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA7120.12369378838286008No Hit
ATCTCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG6190.10753715591150334No Hit
ATCTCTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT6150.10684224698800414No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG6120.10632106529537974No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCTGA6060.1052787019101309No Hit
ATCTCTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5950.10336770237050806No Hit
ATCTCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5790.10058806667651121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG150.00715122852.1229227
AACGCTC150.0071561652.11383424
TCCGCTA150.00719570752.0412651
CGTACGG150.00719570752.0412651
TCGTTGA251.6464099E-552.04125650
AATATCG150.007208098752.01862346
ATATCGG150.007208098752.01862347
AACTCGA307.9684287E-752.01862343
ACGAAGT150.007208098752.01862347
CGCACTC353.873356E-852.00956739
CCGTAAT150.007215541352.00504728
CGTCAAC150.007215541352.00504731
TGCCGAT150.007215541352.00504734
TACACTA401.904482E-951.96436318
TCGTAAT150.007247858751.9463119
CCAACGA203.4668093E-451.93728320
ATCTCTG597600.050.0252651
TCTCTGG597650.050.0210842
CTCTGGG598050.049.9876253
TCTGGGG285250.049.8160934
Produced by FastQC (version 0.11.5)