FastQCFastQC Report
Tue 31 May 2016
SRR212764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572767
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA52720.9204440898305943No Hit
ATCTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33420.5834833361558889No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA30750.5368675220464866No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG24760.43228747466247186No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA23910.4174472342156584No Hit
ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22040.3847987052326688No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA12840.22417492627892321No Hit
ATCTCTGGGGGTGTTCGTCAAGGTCTGAGATTGACAAGGGGCTCCCTTCCCCATCTCT12650.22085769606140018No Hit
ATCTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10440.18227307089968522No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9690.16917874109367334No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA9170.16010000576150513No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA9040.15783032192846305No Hit
ATCTCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8470.14787863127589404No Hit
ATCTCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC7850.13705398530292423No Hit
ATCTCTGGGACCAAGGGATCTACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGA7450.1300703427397179No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT6840.11942028783082824No Hit
ATCTCTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5950.10388168312769415No Hit
ATCTCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5910.10318331887137352No Hit
ATCTCTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5890.10283413674321322No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG5790.10108822610241162No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGA150.007196970752.03890625
CGAACGT150.00720445552.0252329
TCGAACG150.00720445552.0252328
TGCGGTA150.007216940652.00245344
ACTCGAT307.9854544E-752.00245344
CGATCGT203.4453935E-452.00245352
TTCGTAA307.990275E-751.997943
TCGTTGA353.8853614E-851.988850
CCCGTCG203.4528467E-451.97969435
CGTATGG150.00724697351.9478747
CGATCAT150.00728967851.87074331
TCTGGGA220600.049.816454
ATCTCTG597150.049.729551
TCTGGGG289950.049.72024
CTGGGAT76250.049.6525
TCTCTGG598900.049.5842362
CTCTGGG599000.049.575963
CTGGGGG122650.049.2266245
CTGGGAG69750.048.648465
TGGGATT31950.048.5950626
Produced by FastQC (version 0.11.5)