FastQCFastQC Report
Tue 31 May 2016
SRR212763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587847
Sequences flagged as poor quality0
Sequence length56
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC92521.573878917473424No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC45380.7719695771178554No Hit
ATCTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38840.6607161387231711No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC26920.4579422877041135No Hit
ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25130.42749218759303015No Hit
ATCTCTGGGGGTGTTCGTCAAGGTCTGAGATTGACAAGGGGCTCCCTTCCCCATCT12120.2061760968415251No Hit
ATCTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11190.19035565376705163No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT10650.18116959004638963No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT10530.17912824255290918No Hit
ATCTCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9330.1587147676181047No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA9170.1559929709601308No Hit
ATCTCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA8540.14527589661935844No Hit
ATCTCTGGGACCAAGGGATCTACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGC6950.11822804233074254No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC6660.11329478588816477No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA6400.10887186631895714No Hit
ATCTCTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC6360.10819141715446366No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC6140.10444894674974951No Hit
ATCTCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT6120.10410872216750278No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCT5950.10121681321840546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG204.0551578E-450.31355350
CGTTAGC150.00826236250.2531343
CTCGCAC150.00830727750.18425839
GTCGTAT150.00832980250.1498938
AATGTCG252.0516722E-550.14130437
GTCCGGC150.00839765150.0470732
ACACGAT150.00842888550.00008829
CGGCACA150.00844880749.97023426
ACGAGAC150.00846020649.9531925
GACTCGT150.00854032149.8342179
ATCTCTG610550.047.9773141
CTCTGGG610750.047.96163
TCTCTGG611300.047.9184532
TCTGGGA241400.047.6769334
TCTGGGG285650.047.636034
CTGGGAT86250.047.2052735
CTGGGGG122750.047.1141365
CTGGGAG73900.046.9682435
TCTGGGT44450.046.8071634
TGGGATT34450.046.7964256
Produced by FastQC (version 0.11.5)