FastQCFastQC Report
Tue 31 May 2016
SRR212762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535371
Sequences flagged as poor quality0
Sequence length56
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC84881.5854426182964712No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC42430.7925345227888698No Hit
ATCTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33660.6287228856251086No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC23950.44735333068096705No Hit
ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23890.44623261252477253No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT11300.2110685860832955No Hit
ATCTCTGGGGGTGTTCGTCAAGGTCTGAGATTGACAAGGGGCTCCCTTCCCCATCT11220.2095742952083695No Hit
ATCTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10360.19351066830291516No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT10060.18790707752194274No Hit
ATCTCTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC8810.1645587826012242No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA8770.1638116371637612No Hit
ATCTCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA8400.15690054186722852No Hit
ATCTCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8050.15036301928942733No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA6970.1301900924779265No Hit
ATCTCTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC6260.1169282609629584No Hit
ATCTCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5880.10983037930705995No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCT5820.10870966115086547No Hit
ATCTCTGGGACCAAGGGATCTACAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGC5790.10814930207276824No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC5690.10628143847911074No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC5630.10516072032291626No Hit
ATCTCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5470.10217213857306429No Hit
ATCTCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5410.10105142041686979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATG150.00826613950.24670446
ACCGATC150.00830929750.1805540
CTAGGTA150.00831547750.17111639
AACACGC150.00831547750.17111639
ATTCGTT204.1310655E-450.12399335
ATTGGCG150.00837745650.0769533
CGCGTAT150.0084335349.99250428
CCGCGTA150.00843977849.9831427
CGATACT150.00848674649.9130123
AACCGAC252.1122272E-549.89434422
CTCCGAT150.00853705949.8384213
CGACGGA150.00853705949.8384215
CGTAGTT150.00853705949.8384212
TATTCGT150.00853705949.8384211
ACGAACG150.00853705949.8384210
ATCTCTG553800.048.1780431
TCTCTGG555250.048.0522272
CTCTGGG555350.048.0435753
TCTGGGA210600.047.7914054
TCTGGGG268000.047.6905364
Produced by FastQC (version 0.11.5)