FastQCFastQC Report
Tue 31 May 2016
SRR212761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349959
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76062.1733974551304582No Hit
AGGGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55581.5881860446509448No Hit
AGGGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25930.7409439391471573No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT22200.6343600250315037No Hit
AGGGTCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17920.5120599841695742No Hit
AGGGTCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17350.49577236190525176No Hit
AGGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9300.26574541589157585No Hit
AGGGTCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7270.2077386208098663No Hit
AGGGTCGGGATGTCACCCCAGCCAAGCAAAAGCTCAGCGTGGGAACCCTGGGACCTTC6340.1811640792207087No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6080.17373463748610551No Hit
AGGGTCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5590.15973299729396873No Hit
AGGGTCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGACA4990.14258813175257673No Hit
AGGGTCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.14058789743941433No Hit
AGGGTCGGGGTCTCAGAGGGGTATAAACGACCCCAAGGATTTCAGCTTGCTCTCCCCA4620.13201546466871833No Hit
AGGGTCGGGAGGAGAGATTCCACGCACTGGGCCCAATTTACTACCGAGATTCGAACGG4550.13001523035555593No Hit
AGGGTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4350.12430027517509193No Hit
AGGGTCGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAG4150.11858531999462793No Hit
AGGGTCGGGGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTC4130.11801382447658153No Hit
AGGGTCGGGAGGATACCTTAGGAGTGCTTTAATGTCTGTCATTATTAGGGCTTCTATT4110.11744232895853515No Hit
AGGGTCGGGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGG4110.11744232895853515No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA3940.11258461705514074No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCT3930.11229886929611756No Hit
AGGGTCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3910.11172737377807115No Hit
AGGGTCGGGGTAAGAGACTGAGGAAAAGGACTAAGTGTAATGTGGGGTCTGTTTTAAA3850.11001288722393196No Hit
AGGGTCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3730.10658391411565356No Hit
AGGGTCGGGGGGATCTGAGAGAGGAAACCTTAAACACATGGGGATAGAAAGAGAAAGG3730.10658391411565356No Hit
AGGGTCGGGATACTAATTGTCTCTTGCTGCACAAGAGCAGTGTCCCTCATGGAAGGAC3510.10029746341714316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACT150.007185557452.05554246
TCTATAC150.007185557452.05554247
AGATAGC150.007189626352.04808844
TTCGTCA150.007201842552.02575337
ACAGGCG150.007201842552.02575342
TACCGGG150.007201842552.02575342
CGAACTA150.007209995352.0108735
CGCTATG150.007209995352.0108734
TTGCGGG203.4407934E-452.01086832
CGCGAAC307.983308E-751.99625
TTACGTA150.00721815551.99626
CGACTAT150.00721815551.99624
AACGATA203.4456557E-451.99599526
CGGATAA203.4480888E-451.98856423
AACGCGA505.456968E-1251.98856423
TCTGTCG203.4505234E-451.98113322
TATTCGT203.4553965E-451.96627415
TCGCATT150.00723858451.95885511
TTTCGCA150.00723858451.95885513
TCGGACC150.00723858451.95885512
Produced by FastQC (version 0.11.5)