FastQCFastQC Report
Tue 31 May 2016
SRR212760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348957
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73252.0991124981014853No Hit
AGGGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54711.5678149456809862No Hit
AGGGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26180.7502357023931315No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT20710.5934828646509455No Hit
AGGGTCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17940.5141034568729098No Hit
AGGGTCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16950.485733199219388No Hit
AGGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9220.264215934914617No Hit
AGGGTCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7150.2048963052754351No Hit
AGGGTCGGGATGTCACCCCAGCCAAGCAAAAGCTCAGCGTGGGAACCCTGGGACCTTC6530.18712907320959316No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6170.17681261588103978No Hit
AGGGTCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5330.15274088211441525No Hit
AGGGTCGGGGTCTCAGAGGGGTATAAACGACCCCAAGGATTTCAGCTTGCTCTCCCCA4920.14099158349022944No Hit
AGGGTCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGACA4600.13182139919818203No Hit
AGGGTCGGGAGGAGAGATTCCACGCACTGGGCCCAATTTACTACCGAGATTCGAACGG4490.1286691483477907No Hit
AGGGTCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4430.1269497387930318No Hit
AGGGTCGGGAGGATACCTTAGGAGTGCTTTAATGTCTGTCATTATTAGGGCTTCTATT4420.12666317053390533No Hit
AGGGTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4280.12265121490613456No Hit
AGGGTCGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAG4020.11520044016884601No Hit
AGGGTCGGGGGGATCTGAGAGAGGAAACCTTAAACACATGGGGATAGAAAGAGAAAGG3960.11348103061408712No Hit
AGGGTCGGGGTAAGAGACTGAGGAAAAGGACTAAGTGTAATGTGGGGTCTGTTTTAAA3950.11319446235496064No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCT3830.10975564324544286No Hit
AGGGTCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.10660339239505154No Hit
AGGGTCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3660.10488398284029266No Hit
AGGGTCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT3640.10431084632203967No Hit
AGGGTCGGGGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTC3620.10373770980378673No Hit
AGGGTCGGGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGG3620.10373770980378673No Hit
AGGGTCGGGGCTTAGGAGGGCACTGGTGGCCCTGTTAACAGCCTGGCATGGGGCAGTG3540.10144516373077485No Hit
AGGGTCGGGGAGGGGATGCTTGGATATTTGCAATGCAGCCCTCCTCTGGGCTGCCCTG3520.10087202721252189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTA150.007134446452.14955524
CGTAAGT150.007134446452.14955527
CGCGTAA150.007134446452.14955525
TCGCGTA150.007134446452.14955524
TCGGTAG150.007134446452.14955525
GCGTAAG150.007134446452.14955526
AACGACC458.913048E-1152.1495526
ATCGTTC150.00713850552.14205623
CTCGACG203.3982482E-452.14205622
CCAACGG203.3982482E-452.14205621
TCACGCG150.007203673552.02237751
CTCACGC150.007203673552.02237750
AACGCCT150.007211850552.00745849
CTACGCG150.00722412951.98509244
ACGTCAA150.00722412951.98509246
CTGTTAT150.007228225551.97764236
CGAGCAC150.007228225551.97764233
ACGTACC150.007228225551.97764232
ATCGATA150.007228225551.97764232
TACTACC750.051.9776439
Produced by FastQC (version 0.11.5)