FastQCFastQC Report
Tue 31 May 2016
SRR212759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212759_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346814
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67771.9540733649737323No Hit
AGGGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48801.407094292617945No Hit
AGGGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22430.6467443644143547No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT18680.5386172415185085No Hit
AGGGTCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15870.45759398409522106No Hit
AGGGTCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15860.45730564510083216No Hit
AGGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7940.228941161544805No Hit
AGGGTCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6300.1816535664650216No Hit
AGGGTCGGGATGTCACCCCAGCCAAGCAAAAGCTCAGCGTGGGAACCCTGGGACCTTC5730.16521824378485298No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5300.15281966702612929No Hit
AGGGTCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4630.1335009544020714No Hit
AGGGTCGGGGTCTCAGAGGGGTATAAACGACCCCAAGGATTTCAGCTTGCTCTCCCCA4290.12369742859284803No Hit
AGGGTCGGGAGGAGAGATTCCACGCACTGGGCCCAATTTACTACCGAGATTCGAACGG4010.11562393674995819No Hit
AGGGTCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGACA3940.11360556378923574No Hit
AGGGTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3900.11245220781168003No Hit
AGGGTCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3900.11245220781168003No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCT3880.11187552982290219No Hit
AGGGTCGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAG3710.1069737669182905No Hit
AGGGTCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.10524373295195696No Hit
AGGGTCGGGGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTC3550.10236034300806773No Hit
AGGGTCGGGGTAAGAGACTGAGGAAAAGGACTAAGTGTAATGTGGGGTCTGTTTTAAA3550.10236034300806773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC1150.052.3511436
ACGATAT203.4335227E-452.03299327
AACGACC750.052.03299326
TCGCGGA203.4335227E-452.03299325
TGCTCGT150.00719781452.0329925
ACCCGGT150.00719781452.0329926
GTGGGCG150.007210178852.01041434
GCATACT150.007210178852.01041436
TGCGGGT203.4458053E-451.99537733
CGAAATC251.6578948E-551.9728344
GTAAGCC150.00723082251.97282841
GGTAATA150.007239091751.9578123
CTACTAC150.007239091751.9578123
TCGACAC203.4605907E-451.95030647
AAACGCC150.00724322951.95030248
CTCGACA203.4630595E-451.942846
CCGTTAC203.4630595E-451.942846
ATCTCGA150.007268090351.9053132
ACGCTGC150.007268090351.9053111
GTGCGAT401.9190338E-951.9053131
Produced by FastQC (version 0.11.5)