FastQCFastQC Report
Tue 31 May 2016
SRR212758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341875
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73222.1417184643510057No Hit
AGGGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53341.5602193784277878No Hit
AGGGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25530.746764168190128No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT22370.6543327239488117No Hit
AGGGTCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16840.4925776965265082No Hit
AGGGTCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16270.47590493601462525No Hit
AGGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8930.26120658135283364No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6920.20241316270566728No Hit
AGGGTCGGGGGGATCTGAGAGAGGAAACCTTAAACACATGGGGATAGAAAGAGAAA6500.19012797074954296No Hit
AGGGTCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6410.18749542961608776No Hit
AGGGTCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5720.1673126142595978No Hit
AGGGTCGGGATGTCACCCCAGCCAAGCAAAAGCTCAGCGTGGGAACCCTGGGACCT5250.15356489945155394No Hit
AGGGTCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.12285191956124314No Hit
AGGGTCGGGAGGAGAGATTCCACGCACTGGGCCCAATTTACTACCGAGATTCGAAC4010.11729433272394882No Hit
AGGGTCGGGGTCTCAGAGGGGTATAAACGACCCCAAGGATTTCAGCTTGCTCTCCC3990.116709323583181No Hit
AGGGTCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3990.116709323583181No Hit
AGGGTCGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAA3650.10676416819012796No Hit
AGGGTCGGGGTAAGAGACTGAGGAAAAGGACTAAGTGTAATGTGGGGTCTGTTTTA3560.10413162705667277No Hit
AGGGTCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3470.10149908592321756No Hit
AGGGTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3440.10062157221206582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG150.00827299150.23224349
CTCGCGA150.00827299150.23224348
ATCGCTC150.00827299150.23224348
TCGCGTC150.00827299150.23224350
TCGCGAC150.00827299150.23224349
CGAAATC150.00828745950.2100444
CTAATCG150.00828745950.2100445
CGCTTAG150.00828745950.2100444
TACCCGT150.00836979950.0846335
TGCGATC204.1540063E-450.0625632
AGTGCGA204.1690818E-450.0258330
TGCGTAG150.00840875750.0258328
AACGATA150.00842828649.9964826
ACGGACA150.00843317349.98914725
CGACTAT252.0906607E-549.9744924
ACGGCTC150.0084723549.9305722
CATCGTT204.208481E-449.9305722
CGTTCAC204.2176154E-449.90863821
TCGTTCA204.2237132E-449.89402820
TTCGATT204.2328736E-449.87212819
Produced by FastQC (version 0.11.5)