FastQCFastQC Report
Tue 31 May 2016
SRR212757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310550
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63512.0450813073579135No Hit
AGGGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46591.5002415070037032No Hit
AGGGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22260.7167927869908227No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT19540.6292062469811625No Hit
AGGGTCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15110.4865561101271937No Hit
AGGGTCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14820.4772178393173402No Hit
AGGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7860.2530993398808566No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6670.21478022862663018No Hit
AGGGTCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6280.2022218644340686No Hit
AGGGTCGGGGGGATCTGAGAGAGGAAACCTTAAACACATGGGGATAGAAAGAGAAA5890.18966350024150702No Hit
AGGGTCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.15424247303171792No Hit
AGGGTCGGGATGTCACCCCAGCCAAGCAAAAGCTCAGCGTGGGAACCCTGGGACCT4250.13685396876509417No Hit
AGGGTCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.12687167927869908No Hit
AGGGTCGGGGTCTCAGAGGGGTATAAACGACCCCAAGGATTTCAGCTTGCTCTCCC3770.12139752052809533No Hit
AGGGTCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.11753340846884561No Hit
AGGGTCGGGAGGATACCTTAGGAGTGCTTTAATGTCTGTCATTATTAGGGCTTCTA3490.11238125905651264No Hit
AGGGTCGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAA3380.10883915633553373No Hit
AGGGTCGGGATCAGCTGAGTCCCACATCCAGGTGACTCTGTGTGAGGCTGGTAAAC3280.10561906295282564No Hit
AGGGTCGGGAGGAGAGATTCCACGCACTGGGCCCAATTTACTACCGAGATTCGAAC3280.10561906295282564No Hit
AGGGTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3230.10400901626147158No Hit
AGGGTCGGGGTAAGAGACTGAGGAAAAGGACTAAGTGTAATGTGGGGTCTGTTTTA3110.10014490420222186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGCT150.00825322850.2615350
AAGTCGT204.0892424E-450.22074541
ACTGCGT150.0082797650.2207447
CTGTACG150.0082797650.2207442
CTGCGTA150.0082797650.2207448
ATTAGCT150.00830103250.18815636
ATAGACC150.00838652450.05824331
CTATTAC150.00838652450.05824331
ACGATAT204.1751197E-450.00969727
TCATCGA150.00843490149.98546226
AACGATA204.18512E-449.9854626
AGTAAAC252.0894919E-549.9773925
CAATAGG150.0084402949.97738625
CTAGCGT252.1015021E-549.92923
AACGCGA301.0556741E-649.92899723
TATCCGT150.00848889649.9048422
CGTACAT355.3863914E-849.86462820
ACACTAT150.0085159949.86462420
CGGGCAT2550.049.816466
CGGGTAT750.049.8164566
Produced by FastQC (version 0.11.5)