FastQCFastQC Report
Tue 31 May 2016
SRR212756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421628
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC164063.891107801189674No Hit
GTAATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG33940.8049750016602313No Hit
GTAATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGC33150.7862381056286585No Hit
GTAATGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC21170.5021013784663257No Hit
GTAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC17010.40343620442665096No Hit
GTAATGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT14960.3548151451042151No Hit
GTAATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT13180.3125978350583927No Hit
GTAATGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC11160.2646883034333583No Hit
GTAATGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCC10660.25282950847666663No Hit
GTAATGGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA10240.24286812071304562No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA9540.22626580777367727No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9280.22009923439619758No Hit
GTAATGGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTT9270.21986205849706375No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8880.21061219843084422No Hit
GTAATGGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATAC7580.17977933154344589No Hit
GTAATGGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC6470.15345280673959036No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6460.1532156308404565No Hit
GTAATGGGGAGGAAGGAATTCATGCCTGATAAAATAATCCTCTTCAAAAGTCCACACC5940.14088248408549717No Hit
GTAATGGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAT5700.13519026250628516No Hit
GTAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5670.13447873480888367No Hit
GTAATGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG5630.13353003121234833No Hit
GTAATGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG5490.13020956862447466No Hit
GTAATGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT5430.12878651322967166No Hit
GTAATGGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTAT5220.12380581934786114No Hit
GTAATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT5170.12261993985219198No Hit
GTAATGGGGATAGCTTTCAGCTTTAAAACTATAAACATTAACAAAAATCCCACATTCG5100.12095970855825515No Hit
GTAATGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5010.11882512546605065No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4800.11384443158424015No Hit
GTAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4730.1121842002903033No Hit
GTAATGGGGACACAATAAGAACTAGAAAAACGTCTCACACAGGAAGTGCCCCCAGTAA4650.11028679309723263No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4510.10696633050935897No Hit
GTAATGGGGACCTTCAGGGCTCTGCATTCTGAGCCTAGTTCTGATATCACATCTGGCT4370.10364586792148528No Hit
GTAATGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG4290.10174846072841463No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA4280.10151128482928079No Hit
GTAATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4270.10127410893014696No Hit
GTAATGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4240.10056258123274545No Hit
GTAATGGGGATCACGAGCCAGCTCTTTCGTTCTGGCCCTGGAGCCAGAACTGGAGTCG4220.1000882294344778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTCG459.094947E-1152.0746452
TATTCGG150.00718966152.04989248
AGACCGG307.930339E-752.04989244
CCCATCG150.00718966152.04989243
ATCCGCT150.00720318152.02517336
TTACGCT251.6486658E-552.0251736
CATTGCG150.0072167252.00047331
CGCTAAA150.00722349651.98813229
ATAGGCG203.4571302E-451.96347417
GGACTAA700.051.963478
ATCACGA353.897003E-851.9634710
AAACGGT150.007237063751.9634710
GGACGTA251.6602875E-551.963478
GACCTAC150.007237063751.963479
GTAATGG425000.051.5477681
TAATGGG425200.051.523522
AATGGGG425500.051.4871943
ATGGGGG147850.051.260554
ATGGGGA218000.051.2007064
TGGGGAT72850.051.1431855
Produced by FastQC (version 0.11.5)