FastQCFastQC Report
Tue 31 May 2016
SRR212755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422002
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC158483.7554324387088216No Hit
GTAATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG34210.8106596651200705No Hit
GTAATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGC33420.7919393746949067No Hit
GTAATGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC21390.5068696356889304No Hit
GTAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC17470.41397908066786415No Hit
GTAATGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT13980.33127805081492506No Hit
GTAATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT12790.30307913232638706No Hit
GTAATGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC10970.2599513746380349No Hit
GTAATGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCC10400.24644432964772678No Hit
GTAATGGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA9870.23388514746375608No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA9410.22298472519087587No Hit
GTAATGGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTT9380.2222738280861228No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9310.22061506817503235No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7880.1867289728484699No Hit
GTAATGGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATAC7540.17867213899460194No Hit
GTAATGGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC6450.15284287752190748No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6450.15284287752190748No Hit
GTAATGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT5590.13246382718565317No Hit
GTAATGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG5520.13080506727456268No Hit
GTAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG5480.12985720446822527No Hit
GTAATGGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAT5390.1277245131539661No Hit
GTAATGGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTAT5380.12748754745238172No Hit
GTAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5380.12748754745238172No Hit
GTAATGGGGAGGAAGGAATTCATGCCTGATAAAATAATCCTCTTCAAAAGTCCACACC5350.12677665034762867No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5210.12345913052544774No Hit
GTAATGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5020.11895678219534506No Hit
GTAATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT4870.11540229667157975No Hit
GTAATGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG4730.11208477684939881No Hit
GTAATGGGGATAGCTTTCAGCTTTAAAACTATAAACATTAACAAAAATCCCACATTCG4700.11137387974464576No Hit
GTAATGGGGACACAATAAGAACTAGAAAAACGTCTCACACAGGAAGTGCCCCCAGTAA4700.11137387974464576No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4590.10876725702721789No Hit
GTAATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4420.10473884010028388No Hit
GTAATGGGGACCTTCAGGGCTCTGCATTCTGAGCCTAGTTCTGATATCACATCTGGCT4400.10426490869711517No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA4260.10094738887493425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA203.414183E-452.09520750
CCGTCCG203.4181922E-452.08282526
AGCCCGA150.0071851952.05808346
CGATTCG150.007188565652.051944
AATCGGG150.007188565652.051943
TCGCATA150.007198699852.0333642
CACGACG307.967319E-752.01483537
GACGCAT203.4463615E-451.99632333
ACGTTCC150.007219000351.9963233
TCGAGTA251.654089E-551.9963233
ATAGGCG203.4645677E-451.9408617
ATCGACG150.007249530451.9408616
GGATCGA505.456968E-1251.9285558
GATTCGT353.9177394E-851.9285559
ATCGCGG150.00725632851.92855510
TAATGGG425500.051.501412
AATGGGG425600.051.489313
GTAATGG425650.051.4832651
ATGGGGC32250.051.364994
ATGGGGG141550.051.2315254
Produced by FastQC (version 0.11.5)