FastQCFastQC Report
Tue 31 May 2016
SRR212754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424110
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC145433.429063214732027No Hit
GTAATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG30590.7212751408832614No Hit
GTAATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGC29200.6885006248378958No Hit
GTAATGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC19410.457664285209026No Hit
GTAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC16460.38810685906958103No Hit
GTAATGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT13860.32680200891278205No Hit
GTAATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT13140.3098252811770531No Hit
GTAATGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC10250.24168258234891893No Hit
GTAATGGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA9600.22635636980971918No Hit
GTAATGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCC8950.21103015727051944No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8860.20890806630355332No Hit
GTAATGGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTT8430.19876918723915968No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8350.196882884157412No Hit
GTAATGGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATAC7000.16505151965292023No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6660.15703473155549266No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6350.14972530711372048No Hit
GTAATGGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC5880.13864327650845298No Hit
GTAATGGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAT5350.12614651859187476No Hit
GTAATGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG5330.12567494282143785No Hit
GTAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5140.12119497300228715No Hit
GTAATGGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTAT5100.12025182146141332No Hit
GTAATGGGGAGGAAGGAATTCATGCCTGATAAAATAATCCTCTTCAAAAGTCCACACC5020.11836551837966565No Hit
GTAATGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA4790.11294239701964114No Hit
GTAATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4780.11270660913442268No Hit
GTAATGGGGATAGCTTTCAGCTTTAAAACTATAAACATTAACAAAAATCCCACATTCG4650.10964136662658273No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4600.10846242720049044No Hit
GTAATGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4580.10799085143005352No Hit
GTAATGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG4320.10186036641437361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAA700.052.3522649
AACGGAT503.6379788E-1252.3522610
GATCTAC251.5886277E-552.352269
GGATTCG503.6379788E-1252.352268
GATTCGA203.332221E-452.352269
AACCGGT203.4053228E-452.12267738
ACTAGAC203.4393347E-452.01786435
ACGTATA150.007213947452.00555840
TCGCATA150.00721732251.9994142
ACGTTCG251.6569928E-551.9809750
CTAACGC150.00722745251.9809752
TACGATG150.00722745251.9809751
CCATACG150.00722745251.9809751
TGTCGAT150.00723083151.97482729
CGAAATC150.00724774451.9441347
TTAACGC150.00724774451.9441346
TTGCCGA150.00724774451.9441348
GTCGATA150.00725790651.92572830
GTCGCGA203.4857797E-451.87672417
CGTAAAG150.00728845551.87060521
Produced by FastQC (version 0.11.5)